Colchicine....What's your opinion?

[guest2012y]

I would make sure to get paid for your work,but to kick out some freebies will help keep people backing up your reputation. As long as you don't "sell out" you can stand to make a few bucks for that work. I would wait until the completed work is done before naming it. Then you'll be able to pick out a name suitable to the qualities,history,and work that are part of it. (Your name here....Nepal Temple...Buddha's Purple Spaceship...etc,etc.)

 

[guest2012y]

I think monsanto could use your services.

Hey...this was funny!! Not being a smart ass Mad...I really laughed. Play nice.

 

[mad librettist]

He has me on ignore so he won't be troubled.

I want to point out the identical logic used to subvert precaution in the promotion of gmo.

 

[maharaji]

Some time ago i read an article where DJ Short said he was wondering if his OPT ( i guess ???i can't remenber if he said OPT or the parents or else ...) had been "messed" with colchyne...any opinion on that ( as i just harvested some F13 i'm interested)! The rest is beyond my league!! Cheers

 

[guest2012y]

Check this thread........you'll have to read it,but this may be what you read,,,I dunno. https://www.icmag.com/ic/showthread.php?p=3285812#post3285812

 

[big ballin 88]

+1

And I really agree with this: "I really like the idea of tetraploidy, it really can expand the plant we know today".

I thought about selling the seeds I will make (it will take me about 1-2 years to make the final version) but I will for sure send tons of freebees out to you guys! I wouldn't mind making a bunch of money for all my effort and time and studying tho!

First I will cross a female Thai 4n by a (masculinezed [sic]) female Nepalese 4n. I will take that F1 and self it to the S3 to S5 (selfed generations). Then I will cross the female (Thai 4n x Nepalise 4n) with a (masculinezed [sic]) female Afghan 4n. I will take that F1 and self it to S3 to S5 (depending upon when I see stabilization of traits); I won't go past S5. Once I have the S3 to S5 all female (Thai 4n x Nepalese 4n) x Afghan 4n I will give the seeds away and maybe sell them considering my variety should be WAY better thna most all others in terms of cannabinoids and yield...and much more unique (colors, bud and leaf size, seed size and color, etc).

Anyone want to suggest a name for the variety to be???

Super T.A.N or maybe T.A.N.G.E(Thai,Afghan, Nepalese Genetic Experiments.) Maybe "Traveling Scholar" sorry i'm out of it...

I agree with Capt. about getting paid, since you are putting in the time and effort, not to mention knowledge that very few would understand. Freebies would definelty be good to show people that good things can come from these experiments. Not to mention it would help prove how the plant grows in different situations in regards to different aspects such as plant variation and adaptability to different environments.

Sorry guys all of a sudden i got super sick, couldn't move for about 30 mins, i hate this nausea that comes up. I dont feel like myself so if my paragraphs dont make sense i'm sorry, even this nice indica i have isn't getting rid of it...

I really would like to see how these type's of experiments evolve...

 

[secondtry]

On the topic of measuring leaf stomatal density as a polyploidy assay:

One should be able use the small ($300) or large ($600) microscope from MM ( http://www.microbeorganics.com/#Microscopes_for_sale ) to measure stomatal density.



Here are two sources of info to measure by micron:

1. “Microscope Micron Measuring,”
http://www.microscopy-uk.org.uk/mag/artdec99/cwnano.html


2. “Microscopy Scales: measurement in the micro-small world,”
http://www.microscopy-uk.org.uk/mag/articles/size.html​

Here is info on measuring and qualifying leaf stomata:

Leaf stomatal measurements for testing stomatal openness and for polyploidy assays

1. “Structure of Leaves,”
http://biog-101-104.bio.cornell.edu/BioG101_104/tutorials/botany/leaves.html


2. “Leaf Stomata as Bioindicators of Environmental Change,”
http://www.accessexcellence.org/AE/AEC/AEF/1994/case_leaf.php


3. “How do I measure stomatal density?,”
http://www-saps.plantsci.cam.ac.uk/records/rec54.htm


4. “Stomatal impressions – some simple alternatives,”
http://www-saps.plantsci.cam.ac.uk/osmos/os20.htm#stoma​

Here is info on using stomatal density to find polyploidy: (the fist two are the important ones)

Please note that a plant can be part diploid and part tetraploid at the same time. For example, one branch and buds can be a diploid while the other branches and buds are tetraploid. That is why soaking seeds is the best way to induce polyploidy, it is the most compote method for 100% (or close to it) tetraploids.

1. “Why does polyploidy result in increased stomatal size?,”
http://www-saps.plantsci.cam.ac.uk/records/rec592.htm


2. “Effect of ploidy on stomatal and other quantitative traits in plantain and banana hybrids,”
http://www.springerlink.com/content/l285372263v5x658/


3. “Induction and identification of polyploids in Cattleya intermedia Lindl. (orchidaceae) by in vitro techniques,”
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782000000100017


4. “Physiology of Polyploid Plants: Water Balance in Autotetraploid and Diploid Tomato under Low and High Salinity,”
http://www3.interscience.wiley.com/journal/119641916/abstract​

Here is info on using root squash to count actual chromosome sets to find polyploidy: (the first two are the important ones)

1. “Chromosome Staining Technique in Plants,”
http://people.bridgewater.edu/~lhill/plant%20chromosomes.htm


2. “Step-by-step Instructions for Staining and Studying Plant Chromosomes,”
http://people.bridgewater.edu/~lhill/chromtechnique.htm


3. “High Frequency Production of Colchicine Induced Autotetraploids in Faba Bean (Vicia faba L.),”
http://sciencelinks.jp/j-east/article/200421/000020042104A0629063.php


4. “Comparative Studies on Diploid and Tetraploid Levels of Nicotiana alata,”
http://docs.google.com/viewer?a=v&q=cacheiNRzNGgVhAJ:www.idosi.org/ajps/2(3)09/11.pdf+high+frequency+prodution+of+colchicine+induced+autotetraploids+in+faba+bean&hl=en&gl=us&sig=AHIEtbRQoUQxCsv45RTJwKGfz_7CCivXuw


​

P.S.
I forgot to mention that not only has tetrploidy been found to DRASTICALLY increase cannabinoids but also DRASTICALLY increase other secondary metabolites of cannabis (and many other plants) such as terpenoids, flavinoids, etc!!!

 

[guest2012y]

On the topic of measuring leaf stomatal density as a polyploidy assay:

One should be able use the small ($300) or large ($600) microscope from MM ( http://www.microbeorganics.com ) to measure stomatal density.



Here are two sources of info to measure by micron:

1. “Microscope Micron Measuring,”
http://www.microscopy-uk.org.uk/mag/artdec99/cwnano.html


2. “Microscopy Scales: measurement in the micro-small world,”
http://www.microscopy-uk.org.uk/mag/articles/size.html




Here is info on measuring and qualifying leaf stomata:

Leaf stomatal measurements for testing stomatal openess and for polyploidy assays


1. “Structure of Leaves,”
http://biog-101-104.bio.cornell.edu/BioG101_104/tutorials/botany/leaves.html


2. “Leaf Stomata as Bioindicators of Environmental Change,”
http://www.accessexcellence.org/AE/AEC/AEF/1994/case_leaf.php


3. “How do I measure stomatal density?,”
http://www-saps.plantsci.cam.ac.uk/records/rec54.htm


4. “Stomatal impressions – some simple alternatives,”
http://www-saps.plantsci.cam.ac.uk/osmos/os20.htm#stoma






Here is info on using stomatal density to find polyploidy: (the fist two are the imporant ones)

Please note that a plant can be part diploid and part tetraploid at the same time. For example, one branch and buds can be a diploid while the other branches and buds are tetraploid. That is why soaking seeds is the best way to induce polyploidy, it is the most compote method for 100% (or close to it) tetraploids.

1. “Why does polyploidy result in increased stomatal size?,”
http://www-saps.plantsci.cam.ac.uk/records/rec592.htm


2. “Effect of ploidy on stomatal and other quantitative traits in plantain and banana hybrids,”
http://www.springerlink.com/content/l285372263v5x658/


3. “Induction and identification of polyploids in Cattleya intermedia Lindl. (orchidaceae) by in vitro techniques,”
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782000000100017


4. “Physiology of Polyploid Plants: Water Balance in Autotetraploid and Diploid Tomato under Low and High Salinity,”
http://www3.interscience.wiley.com/journal/119641916/abstract​

Here is info on using root squash to count actual chromosome sets to find polyploidy: (the first two are the important ones)

1. “Chromosome Staining Technique in Plants,”
http://people.bridgewater.edu/~lhill/plant%20chromosomes.htm


2. “Step-by-step Instructions for Staining and Studying Plant Chromosomes,”
http://people.bridgewater.edu/~lhill/chromtechnique.htm


3. “High Frequency Production of Colchicine Induced Autotetraploids in Faba Bean (Vicia faba L.),”
http://sciencelinks.jp/j-east/article/200421/000020042104A0629063.php


4. “Comparative Studies on Diploid and Tetraploid Levels of Nicotiana alata,”
http://docs.google.com/viewer?a=v&q=cacheiNRzNGgVhAJ:www.idosi.org/ajps/2(3)09/11.pdf+high+frequency+prodution+of+colchicine+induced+autotetraploids+in+faba+bean&hl=en&gl=us&sig=AHIEtbRQoUQxCsv45RTJwKGfz_7CCivXuw

​

P.S. I forgot to mention that not only has tetrploidy been found to DRASTICALLY increase cannabinoids but also DRASTICALLY increase other secondary metabolites of cannabis (and many other plants) such as terpenoids, flavinoids, etc!!!

Whoa!!! Got some reading to do here for sure,thanks for the sources Sec.

 

[secondtry]

Super T.A.N or maybe T.A.N.G.E(Thai,Afghan, Nepalese Genetic Experiments.) Maybe "Traveling Scholar" sorry i'm out of it...

I agree with Capt. about getting paid, since you are putting in the time and effort, not to mention knowledge that very few would understand. Freebies would definelty be good to show people that good things can come from these experiments. Not to mention it would help prove how the plant grows in different situations in regards to different aspects such as plant variation and adaptability to different environments.

Sorry guys all of a sudden i got super sick, couldn't move for about 30 mins, i hate this nausea that comes up. I dont feel like myself so if my paragraphs dont make sense i'm sorry, even this nice indica i have isn't getting rid of it...

I really would like to see how these type's of experiments evolve...

Sorry your not feeling well bro, I hope you get better!

I like your thought about TANGE, or maybe TNA-4N, or PTAN (Polypoid-Thai-Nepalese-Afghan)...but I agree with the Capt., I should wait unit it's done.

 

[secondtry]

Here is a pic of what one would draw when counting chromosome sets, this pic is what was drawn when looking at a root squash. To find the level of polyploidy one counts the sets:

 

[secondtry]

Whoa!!! Got some reading to do here for sure,thanks for the sources Sec.

NP

 

[mad librettist]

Dispatch from the solaris mission to research the implosion of The human species on earth: it appears this species was never able to learn from mistakes, and repeatedly convinced itself it could fuck with nature while evading the law of unintended consequences. While the loss was cause for sadness, humanity was a dangerous combination of crafty and fucking stupid. Sentient life everywhere has dodged a phaser blast.

 

[big ballin 88]

Dispatch from the solaris mission to research the implosion of The human species on earth: it appears this species was never able to learn from mistakes, and repeatedly convinced itself it could fuck with nature while evading the law of unintended consequences. While the loss was cause for sadness, humanity was a dangerous combination of crafty and fucking stupid. Sentient life everywhere has dodged a phaser blast.

Tell that to the people who turned purple carrots, orange!! lol sorry i couldnt resist...

 

[secondtry]

Hello and welcome to IC!

Some time ago i read an article where DJ Short said he was wondering if his OPT ( i guess ???i can't remenber if he said OPT or the parents or else ...) had been "messed" with colchyne...any opinion on that ( as i just harvested some F13 i'm interested)! The rest is beyond my league!! Cheers

I have no idea, but I think most often when someone says variety X is a polyploid I tend not to believe them...

If someone wants to send me clones of Oregon Purple Thai I could check it out. What is the heritage of the F13?

HTH

 

[secondtry]

Tell that to the people who turned purple carrots, orange!! lol sorry i couldnt resist...

Haha. Why doesn't MadL just go home if he doesn't like this topic? The only time i can see his posts are when someone quotes them...stop quoting him! (kidding).

What I find odd with the likes of MadL's opinion is "autotetraploidy" is common in the genetic history of almost all higher plants, and it has been shown that CANNABIS has polyploidy in it's rather recent history...so it's not like I would be doing something unknown to cannabis (ie. chromosome set doubling)...

P.S. BB88, you asked me about AM fungi by PM right? If that was you I will send you a PM very soon, I got stuck on this topic and the Co2 thread of Lazyman today (in the grow room design and equipment sub-forum), sorry!

 

[secondtry]

P.S. BB88:

If the indica isn't helping you could just send it to me!

 

[guest2012y]

Dispatch from the solaris mission to research the implosion of The human species on earth: it appears this species was never able to learn from mistakes, and repeatedly convinced itself it could fuck with nature while evading the law of unintended consequences. While the loss was cause for sadness, humanity was a dangerous combination of crafty and fucking stupid. Sentient life everywhere has dodged a phaser blast.

All I can say is that must be some good smoke Mad. Is that indica dominant? Kind of looks 50/50.........

 

[secondtry]

When I do start selling my seeds I would always offer them for free (or big discount) to medial growers and caregivers (one pack each of 5-10 seeds only so I don't get taken advantage of).

I assume I would charge $2.50 to $5 per seed, so that would be $25 or $50 for a pack of 10 seeds which are all female and better than seeds other breeders charge $15-20 each (ie. for all female seeds today one will pay $150-200 per pack of 10 seeds). And fuck Sensi Seeds and their $30 seeds!!!

 

[mad librettist]

All I can say is that must be some good smoke Mad. Is that indica dominant? Kind of looks 50/50.........

Lol cheese I speak with the zeal of a convert, that's true.

My sense of blue Burmese is sativa high with an indica finish. I need coffee or tea or more smoke after an hour.

 

[big ballin 88]

P.S. BB88:

If the indica isn't helping you could just send it to me!

I didn't send you a message on AM, must've been someone else.

This indica i am in love with! We really don't get a lot of them here and the person who gave me the buds doesn't know the grower sadly. I should take some pics of it just so you can see why i want it(Sorry it didnt come out well) It smells of rip fruit and went good with the last of my hash, sadly.

Luckily, in my search for an indica i went with Grindhouse's Pre98bubba x TangTang.