led05
Chasing The Present
now you've got me wondering about the Waxy Male Gambian I've been using..... The Gambian line is extremely interesting & rare, around 15-20% of seeds have no serrations and are dead ends IMO, horrible transpiration without them serrations, I've put up pics in here....... Some love the look of them as they don't look a ton like cannabis without serrations, so in illegal places...lol this second mutant is most likely a tetraploid. did it have a thick tumor like tap root? the red xylem is an excellent selection trait/ plant to watch. i like to germinate my seeds a little extra longer so i can see which ones sprout with the thick red stem, curved/twisted true leaves, fused cotyledons and best of all an abnormal sized endosperm cap. its significant that all instances of polyploid/aneuploid dicoveries in cannabis happen in the highest altitude regions of india/himalaya. a well known location type for natural hybrids and extreme adaptation.
for example the only natural tetraploid population so far was found in the cold desert shadow of lahu/spiti. this unique climate is known to produce polyploids in normal diploid species due to their larger cell size helping survive the brutal conditions.
the slides i just pasted are aneuploids from a study in himchal pradesh(shimla/kullu) these findings were in tune with your plant. abnormal large genomes, extra chromatin, cytomixis(another nuclei enters a cell). in some cases 7 nuclei were found fused together.
the most recent finding was a triploid male in semi feral uttakarhand. red xylem is caused by a null CAD enzyme in the lignin metabolism. the CAD enzyme is supposed to change the aldehydes into alcohols and then send them on to create lignin for cell wall strength. when the cad enzyme is null the aldehydes can no longer be cganed or used for lignin and instead the pre cursor flows into the phenylpropanoid pathway.
this pathway is responsible for synthesizing the direct pre cursor of cbga and phytohormones. even further, the null enzyme causes an abundance of hydroxycinnamates which are incorporated right into the lignin causing the "weeping" red xylem phenotype. this change means the stems take on heavy mentholated comounds, so the red doesnt just look cool its functional.
but this new red lignin has some defects- its not as tight as regular lignin and its building blocks contain errors in contruction such as the floating node extra digits etc. its very possible the cytomixis observed is facilitated by this irregular cell wall construction.
generally the darker the red the more additional phenols are present. this null enzyme is most likely passed on from a wild mulberry from yunnan- morus notabils. this is the closest genome to cannabis and has enzymes which nearly perfect match to thc synthase. so the red xylem may be at the very heart of potency/acclimation. some reading if you like. super cool man
tetraploid from cold desert https://www.eresearchco.com/article...t-higher-altitude-himalayasa-neglected-bu.pdf
uttakarhand triploid- https://www.mdpi.com/2223-7747/11/20/2736
aneuploid from shimla
View attachment 18827058
red mulberry xylem
A Century-Old Mystery Unveiled: Sekizaisou is a Natural Lignin Mutant
Sekizaisou, a red-wood mulberry variety used in traditional sericulture, is a naturally occurring lignin mutant.www.ncbi.nlm.nih.gov
View attachment 18827062
another 15-20% are just duds, glad I made thousands from the original bulking, which I've gone though a lot of seeds to find ONE (over a thousand) high quality and amazing male, but I did, and he's a super stud being put through the ringer around here lately & tested on many types of plants.....
Here's the Waxy Male Gambian up close, this fella just oozes the goods, so much so he's truly "waxy", for a pure NLD, that's somethin IME
I've been doing a lot of work with these Cambodians x Gambians, and have a few iterations young seedlings going now of crosses with both Gambian Male and Cambodian / Laos Male, some of the offspring are very hard to get seeds to form, others make thousands like most plants..... I selfed both Cambodian #1 and #2 and made a good amount of seeds, then crossed that to my best hazes, other NLD, but lately focus has been with the male Gambian just mentioned......
while many places keep breeding the same few plants or selfing off springs of a few, I feel like I'm swimming in endless piles of genetic renaissance, perhaps I should start making things available to more others without being so anal & perfectionist about it, after all - many of us are looking for variations of similar things and not just variations of cake.... ha
Great info & TY again, I'll dive deeper as soon as time permits, lot of plantings to do today and caring for hundreds of others up and going.... I love spring
Peace
talk to me more about your Laos male, terps, effects, did you smoke him, sniff any pollen..? do you know which batch it was from, they are also from Laredo travel too, right, your Laos...?
Cheers & TY buddy
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