dizzlekush
Member
BAP Stock Solution Formulas
BAP Stock Solution Formulas
I found a few different instructions to making BAP stock solutions. For my testing i would like a 10,000ppm (1.0%) BAP stock solution so i could easily control ppm for testing
examples
5ml BAP stock solution + 995ml combined water, saponins, and surfactant =
50ppm BAP spraying solution
2.5ml BAP stock solution + 997.5ml combined water, saponins, and surfactant =
25ppm BAP spraying solution
10ml BAP stock solution + 990ml combined water, saponins, and surfactant =
100ppm BAP spraying solution
anyway here they are:
BAP Stock Solution Formulas
I found a few different instructions to making BAP stock solutions. For my testing i would like a 10,000ppm (1.0%) BAP stock solution so i could easily control ppm for testing
examples
5ml BAP stock solution + 995ml combined water, saponins, and surfactant =
50ppm BAP spraying solution
2.5ml BAP stock solution + 997.5ml combined water, saponins, and surfactant =
25ppm BAP spraying solution
10ml BAP stock solution + 990ml combined water, saponins, and surfactant =
100ppm BAP spraying solution
anyway here they are:
http://www.patsnap.com/patents/view/EP1579768A1.html0032]From the above description it can be inferred that 6-benzyladenine, if applied at suitable times and with suitable doses onto fruitlets and/or unripe young fruits of the actinidia genus, can increase size and weight of ripe fruits and change positively their form, without jeopardizing their firmness and other quali-quantitative parameters.
[0033]The addition of natural substances such as leonardite, and/or its active components, or similar compounds, or of low toxicity natural compounds with surfactant and/or surfactant-tackifying properties, such as ethoxylated sorbitan monolaurate and/or the others as mentioned above, increases the activity of 6-benzyladenine to a significant extent.
[0034]Said natural substances can be added to 6-benzyladenine alone or in any combination.
[0035]For instance, a formulation comprising 6-benzyladenine in association with leonardite, and/or one or more of its active substances, and with ethoxylated sorbitan monolaurate, and/or at least a surfactant and/or surfactant tackifier chosen among those described above, has proved particularly useful.
[0036]The preferred application is spraying in aqueous solution, carried out by means of current equipment normally used for applying plant protection products and/or plant hormones onto plants. Said application is executed one or more times on young fruitlets starting from few days after petal drop until fruits, which are still unripe, have stopped growing and have reached the size of ripe fruits.
[0037]Said composition shall preferably be in liquid form, although solid formulations are not at all excluded, such as for instance powders, granulates or pills or water-soluble tablets.
[0038]In case of liquid formulations, 6-benzyladenine and most components listed above will be present in solution; however, some of them that are less soluble in water (leonardite for instance) can be used in suspension.
[0039]Generally speaking, the formulations according to the invention are prepared by dissolving first a suitable amount of potassium hydroxide in water, preferably lukewarm water, so as to reach a basic pH for stabilizing 6-benzyladenine in time; then (if required by the formulation) ethoxylated lauryl sulfate or the desired surfactant and/or surfactant-tackifier is added; 6-benzyladenine is usually added as final component, keeping the solution under stirring. Leonardite, if required, can be added at any time.
[0040]Preferably, 6-benzyladenine is then prepared by solubilization in water, after adding strong bases, in concentrations of 10,000 to 500,000 ppm, preferably of 20,000 to 100,000 with respect to solution volume.
[0041]By mere way of non-limiting example, the following contains some possible examples of concentrated mother liquor, in which the weights of the single components are in grams per 100 grams of composition.
Recipe A
pure 6-benzyladenine 9.35 g;
potassium hydroxide 5.0 g;
water to 100 g.
Recipe B
pure 6-benzyladenine 2.0 g;
monopropylene glycol 20.0 g;
water to 100 g.
Recipe C
pure 6-benzyladenine 9.35 g;
ethoxylated monolauryl sulfate 10.0 g;
potassium hydroxide 5.0 g;
water to 100 g.
Recipe D
pure 6-benzyladenine 9.35 g;
nonyl phenol 4.0 g;
chipped potassium hydroxide 4.25 g;
leonardite 2.0 g;
water to 100 g.
[0042]It should be kept in mind that the concentrations referred to by way of example in the previous recipes as well as those referred to as preferred for 6-benzyladenine refer to concentrated mother liquor.
[0043]More to the point, the low values contained in the table of the experimental tests above are due to the fact that said concentrated mother composition is not used as such but it is diluted before use in water of spraying machines, for instance at a dose of 100 ml per 100 liters of water.
[0044]Suppose for instance a formulation containing 100 g of 6-benzyladenine per liter of solution, corresponding to a concentration of about 100,000 ppm of 6-benzyladenine with respect to solution volume.
[0045]If 100 ml of this concentrated product are diluted in 100 liters of water before use, a dilution of 1,000 will be obtained. The final concentration of 6-benzyladenine will be therefore of 100 ppm.
http://ip.com/patapp/EP1579768A1The percentage ratios of the components of the formulations are to be considered as % by weight with respect to a total weight of 100 grams of final formulation.
In a reactor equipped with stirrer and heating and cooling means, a basic solution is prepared by dissolving under stirring at a temperature of about 35°C an amount of 90% potassium hydroxide, corresponding to 4.25% by weight of the final formulation, in an amount of water of 82.30% by weight.
The solution thus obtained is gradually added under constant stirring with an amount of ethoxylated lauryl sulfate corresponding to 4% by weight of the final formulation.
Eventually, still under stirring, an amount of 98% 6-benzyladenine corresponding to 9.45% by weight of the final formulation is added.
The resulting solution is cooled at room temperature under stirring, then it is packed so as to obtain the desired concentrated mother liquor.
Following the procedure described in Example 1, the following concentrated formulations were prepared.
2)
98% 6-benzyladenine 9.45%;
polyethoxylated alkylaryl ether 4.00%;
humic acids 0.01%;
90% potassium hydroxide 4.25%;
water 82.29%.
3)
98% 6-benzyladenine 9.45%;
ethoxylated sorbitan monooleate 4.00%;
90% potassium hydroxide 4.25%;
water 82.30%.
4)
98% 6-benzyladenine 9.45%;
ethoxylated sorbitan monooleate 4.00%;
humic acids 0.01%;
90% potassium hydroxide 4.25%;
water 82.29%.
5)
98% 6-benzyladenine 9.45%;
amine salt of ethoxylated and
phosphorylated polyaryl phenol 4.00%;
ethoxylated alcohol 3.00%;
90% potassium hydroxide 6.25%;
water 77.25%.
6)
98% 6-benzyladenine 9.45%;
polyethoxylated alkylaryl ether 4.00%;
90% potassium hydroxide 4.25%;
water 82.30%.
http://orchidvault.com/node/70Directions for making BAP Stock Solution (1mg/ml)
100mg BAP (Benzylaminopurine)
1ml 30% KoH (Potassium Hydroxide)
99ml Distilled Water
BAP will not dissolve in water, so KoH is usually used as the solvent. Add the 100mg of BAP to 1ml of KoH. Carefully stir the mixture until the BAP has fully dissolved. The slowly add the 99ml of distilled water.
At this point I usually place 10ml of the stock solution into small vials and freeze any of them that I am not going to immediately use. Then when you need to make a batch of stem prop medium, all you need to do is thaw one vial of the stock BAP solution.
http://patents.com/us-20110160444.htmlPreparation of 6-BA Stock Solution (1 mg/ml):
[0055] 100 mg 6-BA was weighed and dissolved in 1 ml 1 N potassium hydroxide for 5 minutes, then 10 ml distilled water was added for complete dissolution. The resulting solution was brought to 100 ml with distilled water and stored at room temperature for later use.
http://www.faqs.org/patents/app/20100216641The composition of claim 6, wherein the amount of 6-benzyladenine is about 2.0% by weight, the amount of potassium hydroxide is about 1.13% by weight, and the amount of water is about 96.87% by weight.