Reversed Backcrossing:)

[englishrick]

hay col...have you read anything ive wrote that was wrong????,,,,,,,,,,

im trying to make sure all i have writen is 100% accurate

im about to read that book,, Robert W. Allards "Principles of Plant Breeding"....ive never even read 1 page of it yet,,,nither have i read Clarke`s book

 

[GMT]

Hi Rick, you're asking questions that are hard to answer as each time it happens the importance of it will be different, though on the whole unoticable. I'm assuming that you are refering to the Doubling:Halving:Reversing: Deleting of sections of the DNA (not forgetting the typos that also seem to occur) at the point of the haploid cells creation. ie the pollen or the reciprical of it. These not only occur within the genes, but at the borders of genes passing sections of dna from one gene into another (the way in which individual sexes are created from hermaphrodite strains). In most cases, as I said, this will occur without being noticable, but once in a while something will happen that will make a gene containing a slightly different coding, create a completely different observable effect upon what it does, (assuiming that it's either not recessive or the plant receives 2 copies of it). So you can end up with a plant passing on its genes to its offspring, but an important piece of the coding within that gene either having been altered, or having been passed to a neighbouring gene. In the latter case, if the neighbouring gene is active, then there wont be any difference in which gene it currently resides within, however if the gene now containing an important piece of coding is not active, then that coding wont get to issue instructions. Like in a piece of gaming software, if you dont make to past the level 8 boss, you dont get to play level 9 even though the coding is present within the software and you are running the software. (slightly different but you know what I mean). If the code is altered to a point that it either no longer functions correctly or does something new, then it wont matter which genes are active, you will have lost the instruction set within that plant. The hard part is knowing how to pass on instruction sets that you want, and not passing instruction sets that you dont. And the bottom line is that this happens at random within any one generation, and it's not until you have stabalised a line that you can say the odds are that the instruction sets you want passed on, will be. In practice a gene which contains errors will be silenced normally, and the other gene from the other parent will become dominant over it, in S1s you hope to have at least one healthy gene to "take over", by femmed F3s or so, you probably have 2 sets of the same gene in some plants, meaning that any errors that occur will be acted upon even if it creates a weeker or ill or infertile plant as there is no "fall back position to take". Alternatively, 2 altered genes may issue instructions that are beneficial to the plant, and allow it to either outperform or out breed (in practice in nature this is the same thing, though for us isnt necessarily) the original. This is the process of evolution in action. Predicting evolution or future health of populations or even individuals is very tricky science.

 

[Colina]

hay col...have you read anything ive wrote that was wrong????,,,,,,,,,,

im trying to make sure all i have writen is 100% accurate

im about to read that book,, Robert W. Allards "Principles of Plant Breeding"....ive never even read 1 page of it yet,,,nither have i read Clarke`s book

Certainty of 100% accuracy will never be attained by anyone, don't get hung up on that. I would read Clarkes MJ Botany first, but read just as much as you can get your hands on. I once on the advice of Vic High found myself reading an in depth treatise on modern advances in the breeding of chickens, lol. Clarke is my favorite author regarding the subject of cannabis. He along with Sam have an uncanny skill in simplifying and cutting to the chase. I too by the way suffer from reading difficulties, certainly difficulties with reading comprehension. It's no big deal, we may need to read things over and over before it sinks in. I'm still learning something new almost every day from a lot of these guys here. You certainly seem to have come a long way in a short period of time on the boards here Rick, it is clear you have a passion for learning. Stay with it and try to remember that there is no substitute for experience regarding a lot of this stuff. You'll need to follow through with the experiments before reaching any conclusions.

 

[englishrick]

Hi Rick, you're asking questions that are hard to answer as each time it happens the importance of it will be different, though on the whole unoticable. I'm assuming that you are refering to the Doubling:Halving:Reversing: Deleting of sections of the DNA (not forgetting the typos that also seem to occur) at the point of the haploid cells creation. ie the pollen or the reciprical of it. These not only occur within the genes, but at the borders of genes passing sections of dna from one gene into another (the way in which individual sexes are created from hermaphrodite strains). In most cases, as I said, this will occur without being noticable, but once in a while something will happen that will make a gene containing a slightly different coding, create a completely different observable effect upon what it does, (assuiming that it's either not recessive or the plant receives 2 copies of it). So you can end up with a plant passing on its genes to its offspring, but an important piece of the coding within that gene either having been altered, or having been passed to a neighbouring gene. In the latter case, if the neighbouring gene is active, then there wont be any difference in which gene it currently resides within, however if the gene now containing an important piece of coding is not active, then that coding wont get to issue instructions. Like in a piece of gaming software, if you dont make to past the level 8 boss, you dont get to play level 9 even though the coding is present within the software and you are running the software. (slightly different but you know what I mean). If the code is altered to a point that it either no longer functions correctly or does something new, then it wont matter which genes are active, you will have lost the instruction set within that plant. The hard part is knowing how to pass on instruction sets that you want, and not passing instruction sets that you dont. And the bottom line is that this happens at random within any one generation, and it's not until you have stabalised a line that you can say the odds are that the instruction sets you want passed on, will be. In practice a gene which contains errors will be silenced normally, and the other gene from the other parent will become dominant over it, in S1s you hope to have at least one healthy gene to "take over", by femmed F3s or so, you probably have 2 sets of the same gene in some plants, meaning that any errors that occur will be acted upon even if it creates a weeker or ill or infertile plant as there is no "fall back position to take". Alternatively, 2 altered genes may issue instructions that are beneficial to the plant, and allow it to either outperform or out breed (in practice in nature this is the same thing, though for us isnt necessarily) the original. This is the process of evolution in action. Predicting evolution or future health of populations or even individuals is very tricky science.

what im saying is , alleles are coding sequences like in computers 1`s an 0`s,,,,an we make computers!!,,,, An individual's genotype for that gene is the set of alleles it happens to possess,,,,,,, In diploid organisms like cannabis,,,,"two copies of each chromozones",ie "two alleles" make up the individual's genotype,,,,,,we know all this!!



but when it comes to The limiting relative frequency of an event,, over a long series of trials it is the conceptual foundation of the frequency that makes interpretation of probability,,,,,, In my project framework, it is assumed that as the length of the series increases without bound, the fraction of the experiments in which we observe the event will stabilize. This interpretation is often contrasted with Bayesian probability,,,,,,,,

lets take advatage of Bayesian probability

what do you think ?

 

[GMT]

well dna isn't 1s and 0s its Cs As Ts and Gs. In addition, dna isnt the only important factor, but also rna and environment. The conceptual foundations and the interpretations are entirely irrelevant, all that matters are the actualities. Whether we percieve them or not, they exist, even if that existence is merely a quantum possability. But lets not get into the structure of an 11 dimensional universe in this thread, there's another one for that . As you say, the event may well stabalise, however that is not to say that the event will be a beneficial or desirable one. Again, in my opinion, interpretation is irrelevant.

 

[danny karey]

very good read guy's, keep it up, this has got to be one of the most informative threads ive ever read.

I enjoy GMT's, colina, and Ricks post's......Keep'em coming

Danny

 

[englishrick]

here is a lil light entertainment...

this bunch of clowns made rice produce Beta Carotene

im gona join the Circus

Beta Carotene is a terpenoid......


fantastic stuff......
http://www.youtube.com/watch?v=2o7RkJGW10k&feature=related

 

[Forest20]

Al gore must be freaking out... manbearpig sorry had to do that my bad. When you look at it the genes they discuss could in theory clearly happen all ready in so called "mutants". So a tri ploy could be the "key" to this event

 

[englishrick]

im super serial...why is no1 listening to me

what a grate southpark episode that was

 

[englishrick]

http://www.youtube.com/watch?v=dIZpb93NYlw&feature=related

The DNA Replication Song

 

[xinsanewickedx]

well i made it through the first 3 pages but i have to say what i did read was really good stuff,if i had the room i would love to try this out.enjoy the rest of your weekend every one

 

[englishrick]

well i made it through the first 3 pages but i have to say what i did read was really good stuff,if i had the room i would love to try this out.enjoy the rest of your weekend every one

unlucky man,,,you missed the best part

get your head round this bro,,,,it will take 2 seconds to think about but hopefully it might draw you in

Recently, a quantum protocol for Byzantine Agreement was demonstrated experimentally, using a four-photon polarization-entangled state. This shows that the quantum implementation of classical Byzantine Agreement protocols is indeed feasible.

lol,,,,hay GMT do you agree with Byzantine Agreement protocols ?

 

[englishrick]

GMT...i love you man....

when it comes to ReverceBackcrossing,,,,what do you think about?

 

[BENJI]

STS looks very complicated so does reversing using other methods i think ill just stick to finding stud males...

Rick good luck with your project i hope u find what your looking for ill be watching out for your results...

Rick your an inspiration bro after mulling over the idea for a couple of days you have changed my mind im going full throttle into this..

Breeding with males and females is soooo 2008.

A comment that helped change my mind..

im confused on what to buy,,,i feel like buying the GA3 capsules , just because it sounds easy

have you figured out STS?......i just need to see it done once , then il be mixin it in a flash next time,,,,,,,,

it reminds me of the first time i did iso oil ,,in a bowl with a coffee filter,,,,,it took me months to get it together, but once i did it ,i could not believe how easy and primative it was



frigin weird thread,eh?,,,,,,,

i feel like ive smoked a book in the last few days,,,

Rick bro ive done some reading and i think i have STS figured out check this thread from REZ..
http://www.icmag.com/ic/showthread.php?t=58957

And look what i found around the corner from my brothers house...
http://www.acechem.com.au/S.html

I will be trying this out ASAP ill let u know how i go...

 

[englishrick]

an how do you feel about quantitative trait locus?

 

[englishrick]

i sware to god,,,i knew what you were beffore you did

its the only project that matters,,,i think you understand

Rick your an inspiration bro after mulling over the idea for a couple of days you have changed my mind im going full throttle into this..



A comment that helped change my mind..



Rick bro ive done some reading and i think i have STS figured out check this thread from REZ..
http://www.icmag.com/ic/showthread.php?t=58957

And look what i found around the corner from my brothers house...
http://www.acechem.com.au/S.html

I will be trying this out ASAP ill let u know how i go...

 

[BENJI]

i sware to god,,,i knew what you were beffore you did

its the only project that matters,,,i think you understand

Hehe nostradamus style...

First up ill try it on my 94 Jack Herer cutting i was abit lazy before and didnt put much thought into it but now im all for and after reading through this thread again i understand fully were your comin from..
Bro...

 

[xinsanewickedx]

i plan on reading every thing bro,when i get a bigger place this something i would give a shot for sure.

 

[Forest20]

XinsanewickedX I have a strong feeling I well give a quote I read in my that retaliative towards "weed" " - Judge not according to the appearance"(Fortune cookie )

 

[englishrick]

Hehe nostradamus style...

First up ill try it on my 94 Jack Herer cutting i was abit lazy before and didnt put much thought into it but now im all for and after reading through this thread again i understand fully were your comin from..
Bro...

lazy ...lol...comon bej,,,,i remember a 2/3 of months ago......its so weird how you have no ego,,,you have come so far so fast,,,,quicker than me

you have caught upto me and even surpassed me in your production skill

rock on bro