Inexpensive, Wet/Dry, Veg/Flower, 8-Minute CBD Test You Can Do At Home!

[goingrey]

Recently grew out a Ringo's gift CBD hemp bag seed ... View attachment 18798166 did the test with it on the right and gorilla bubble bx1 on the left... good enough for me... View attachment 18798167

Nice looking bud and the result is clear (as the solution isn't). But how do we interpret the test on the left? Half purple half not..

 

[Akss]

The result is up top of the test tube..clear. the bottom is the material and charcoal and lye mixture...well actually it was still a bit cloudy in that pic but it was clear when it settled.. no color change though...

 

[bugman52]

The result is up top of the test tube..clear. the bottom is the material and charcoal and lye mixture...well actually it was still a bit cloudy in that pic but it was clear when it settled.. no color change though...

you testing for cbd or thc? oops you said lye so cbd

 

[Akss]

Yep CBD.. the Ringo's turned pretty dark purple and the GB had no color change which shouldn't just tested it to see the difference..

 

[EastCoastGambit]

Just ordered some materials to give this a go. Because I am growing what I hope is a strain that contains some CBD as its a Thunderfuck mountain x Harlequin cross.

I'm also growing some Bodhi gear Pinesoul and Cocoon.

One of my supposedly Pinesoul plants looks suspiciously a lot like the TFM x H plants and the terps are not tell tale as there is a little similarity between all of them. Hoping this test can help me sort out if I mixed up on my labeling or not? LOL

 

[EastCoastGambit]

Samples are dry

 

[EastCoastGambit]

My results are in and they are.....confusing.

Seems they either all have notable CBD or I messed something up in testing.

I don't know all the strains by # right now because I'm in dark and can't check the tags but I do know 7 & 8 are the harlequin crosses.

 

[EastCoastGambit]

This goes 1 to 8 left to right. 9 is a control as 1st just tested the lye and ISO. It didn't change so I dropped in .035 of Skywalker kush.

I was seeing pinkish reactions with just the sample and 1/2 filled w/ 91% ISO and shake.

 

[EastCoastGambit]

So it seems my Bhodi strains have equal or potentially greater CBD content than the Harlequin crosses.

 

[EastCoastGambit]

Maybe Wookie has some CBD in it?

 

[EastCoastGambit]

Curious to know what 2, 3, & 6 are?

 

[EastCoastGambit]

Took a rip of #8 to start this alchemy as my dried samples are like .1. Good buzz from it

 

[goingrey]

Curious to know what 2, 3, & 6 are?

They seem to have the most CBD. But still not a very dark purple so medium? How's their buzz?

 

[EastCoastGambit]

They seem to have the most CBD. But still not a very dark purple so medium? How's their buzz?

Haven't tried those yet. 6 is Soulmate I confirmed

 

[bugman52]

Curious to know what 2, 3, & 6 are?

those seam to have the hightest CBD

 

[Douglas.Curtis]

I had cross contamination issues a few times. Scissors, and since I only have a couple of them, bottles and caps from quick (meaning incomplete) cleaning between tests.

It does look like you had good results with a lot of cbd in some.

 

[EastCoastGambit]

Yes but just not in the plants I was anticipating. It's actually a mix bag between the 3 strains. I may have some cross contam as well. I tried to rinse my gloves between handling diff samples. All in all its a cool test. It's informative. It didn't help me sort out if I mixed up strains because I was not expecting CBD from Bodhi genetics but definitely was from Harlequin crosses. I guess it's an added bonus that all the plants have some considering my back pain and inflammation issues. The terp profiles on all the plants have similarities as well.

 

[EastCoastGambit]

In case anyone is curious.

1. TFM x HarliQ
2. TFM x HarliQ
3. Soulmate? Looks like other TFM plants
4. Soulmate
5. Cocoon
6. Soulmate
7. TFM x HQ
8. TFM x HQ
9. Skywalker kush

 

[acespicoli]

Fig. 1. Reaction between THC (a) and Fast Blue BB (b) forming the THC + FBBB (c) product chromophore/fluorophore [13].

Fast Blue BB salt

Fast Blue BB salt is used as a general substrate for -glycosidase. Two moles of 6-bromo-2-napthol--d-glucopyranoside are cleaved by -glucosidases into 2 moles of d-glucose and 2 moles of 6-bromo-2-napthol. The 2 moles of 6-bromo-2-napthol formed are coupled with 1 mole of Fast Blue BB salt [4-benzoy

www.thermofisher.com

Chromophores, Fluorophores: properties and characteristics

Peptide-based enzyme substrates linked to chromophores or fluorophores.

www.bachem.com

@Douglas.Curtis got anything for terpenes?
spice ginder and extracted plant material, nice post, hope your well @Douglas.Curtis
Just thinking about you the other day !


anyone have a link to the paper ?
regulated chemicals being the limiter...

 

[acespicoli]

recommended reading

Techniques in Organic Chemistry

Techniques in Organic Chemistry

www.academia.edu

CRC Handbook of Chemistry and Physics - Wikipedia

en.wikipedia.org

google for pdf's
The light you use affects the visible color results? Full sunlight vs narrow spectrum led ?

a variable that needs to be added to results is light source?
think Doug hinted at that in a earlier post, his results favored full spectrum sun light?
Indoors at night with led narrow width....