:D Genetic Preservation :D - Breeding

[acespicoli]

Ancient viruses contributed to the evolution of hemp and marijuana. Summary: THC and CBD, bioactive substances produced by cannabis and sought by medical patients and recreational users, sprung to life thanks to ancient colonization of the plant's genome by viruses, researchers have found.

University of Toronto. "How ancient viruses got cannabis high." ScienceDaily. ScienceDaily, 26 November 2018. <www.sciencedaily.com/releases/2018/11/181126105506.htm>.

 

[acespicoli]

Chinese etymology​

Má (Mandarin pronunciation: [mǎ]), a Chinese word for cannabis, is represented by the Han character 麻.[2][3][4] The term ma, used to describe medical marijuana by 2700 BCE, is the oldest recorded name for the hemp plant.[5]

The word ma has been used to describe the cannabis plant since before the invention of writing five-thousand years ago. Ma might share a common root with the Proto-Semitic word mrr, meaning "bitter."[citation needed] Evidence of the earliest human cultivation of cannabis was found in Taiwan.[6][7][8] Ancient Chinese prose and poems, including poetry in the Shi jing (Book of Odes), mention the word ma many times. An early song refers to young women weaving ma into clothing.[2][9][8]

The word ma is often paired with the Chinese word for "big" or "great" to form the compound word dama or 大麻 (dàmá). Dama is sometimes used to describe industrial hemp, as there is a negative connotation meaning "numbness" associated with the word ma by itself.[10][11] Historical Chinese medical texts (c. 200 CE) through contemporary twentieth century Chinese medical literature discuss individual terms for ma, including mafen (麻蕡), mahua (麻花), and mabo (麻勃), referring to specific parts of the male and female flowers of a cannabis plant with differing cannabinoid ratios.[12]

Yinyang (Yin-yang) | Internet Encyclopedia of Philosophy

iep.utm.edu

 

[acespicoli]

Chinese medical literature discuss individual terms for ma, including mafen (麻蕡), mahua (麻花), and mabo (麻勃), referring to specific parts of the male and female flowers of a cannabis plant with differing cannabinoid ratios.[12]

 

[acespicoli]

Origin and Evolution of the Cannabinoid Oxidocyclase Gene Family

Cannabis is an ancient crop representing a rapidly increasing legal market, especially for medicinal purposes. Medicinal and psychoactive effects of Cannabis rely on specific terpenophenolic ligands named cannabinoids. Recent whole-genome sequencing ...

www.ncbi.nlm.nih.gov

 

[acespicoli]

Variety 1: South Asian domesticate

Morphology​

Plants usually >2.0 m tall (shorter in inhospitable situations).

Central stem (stalk) internodes relatively long (often >12 cm, shorter in shorter plants), somewhat hollow (up to 1/3 stem diameter).

Branches flexible, diverging from the stalk at relatively acute angles (around 45°). Leaf palmately compound, largest leaves typically with at least 7 leaflets, leaflet edges not overlapping.

Central leaflet long and narrow, lanceolate or linear-lanceolate in shape; margins with moderately coarse serrations, and rare secondary serrations. Female inflorescence (and infructescence) elongated and somewhat diffuse, with relatively obscure sugar leaves (a high perigonal bract-to-leaf index).

Sugar leaves with CSGTs limited to the proximal half. Perigonal bract covered with a moderate density of CSGTs. Perianth membranous, hyaline with pigmented areas (brown and mottled or marbled in appearance); mostly sloughed off but sometimes persistent.

Achene, usually ≥ 3.6 mm long, globose to elongate, exocarp green-brown; abscission zone poorly developed.

Phytochemistry​

Dried female inflorescences:
THC ≥0.3%, in late 20th century accessions, nearly always >1.0%; literature weighted x¯ = 3.97%, up to 12.5%.

THC/CBD ratio ≥7, and often >100. THCV is commonly present, especially in landraces from South Asia and Africa. Hillig and Mahlberg (2004) report THCV+CBDV% content x¯ = 0.25%.

Terpenoid profile often imparts an “herbal” or “sweet” aroma, with terpinolene, β-caryophyllene, trans-β-farnesene, and a-guaiene content significantly higher than Central Asian plants.

 

[acespicoli]

Variety 3: Central Asian domesticate​

Diagnosis​

Plants with THC% ≥0.3% in inflorescence and a THC/CBD ratio <7 (almost always >1); central leaflet length:width ratio <6 in fan leaves near the base of inflorescences; mature achenes usually ≥ 3.6 mm long, the perianth mostly sloughed off, lacking a prominent protuberant base, and lacking a well-developed abscission zone that allows easy disarticulation.

Morphology​

Plants usually < 2 m tall, often <1 m. Central stem (stalk) internodes short (often 5–11 cm), mostly solid, central hollow usually less than 20% of stalk diameter. Branches in well-developed plants begin close to ground level, at an angle sometimes nearly 90° from the stalk axis, producing a menorah-shaped habitus.


Leaf palmately compound, largest leaves typically with 7–11 leaflets, leaflet edges often overlapping, color dark green (“black hemp” Vavilov 1992). Central leaflet long and broad, often oblanceolate in shape; margins with coarse serrations, secondary serrations rarely seen.

Female inflorescence (and infructescence) compact, often agglutinated with trichome exudate, with prominent sugar leaves (a low perigonal bract-to-leaf index); short internode length causes axillary racemes become confluent and coalesce into collective congested colas.

Sugar leaves with dense CSGTs on the proximal half, often present beyond the midpoint of the leaflet. Perigonal bract densely covered with CSGTs. Perianth membranous, usually sloughed off, with a fringe of striped or irregularly mottled pigmentation near the base of the fruit.

Achene usually ≥ 3.6 mm long, exocarp green to gray; base blunt and lacking well-developed abscission zone.

Phytochemistry​

Dried female inflorescences:

THC ≥0.3, in late 20th century accessions nearly always >1.0%; literature weighted x¯ = 5.69%, up to 14.5%. This variety expresses the highest total THC%+CBD% (a measure of relative resin content of the plants, since these two cannabinoids usually dominate the resin) of all varieties, which correlates with its dense covering of glandular trichomes.

Its THCV%+CBDV% content is lower than South Asian populations; Hillig and Mahlberg (2004) report a mean of 0.14%.

Terpenoid profile imparts an acrid or “skunky” aroma, and uniquely expresses sesquiterpene alcohols, such as guaiol, γ-eudesmol, β-eudesmol, and the monoterpene alcohol nerolidol, as well as hydroxylated terpenoids, such as γ-elemene, a-terpineol, and β-fenchol.

Genetics​

Allozyme and DNA studies that segregated Central Asian and South Asian domesticates are detailed in the genetics section of Variety 1. Onofri et al. (2015) identified a SNP in the gene that encodes THCA synthase that was unique in two Afghani accessions and a Moroccan “hashīsh landrace” (their SNP accession code no. 1179, A→T transversion). It was not present in 16 other accessions of fiber- and drug-type plants.

Other characters​

Generally early maturing, with greater late-season frost tolerance than South Asian domesticates. Late-season cold triggers anthocyanin production in leaves and inflorescences – the sought-after “purple weed.” Achenes are mostly retained on plants, trapped by surrounding parts of the dense infructescence. Plants are more susceptible to gray mold (Botrytis cinerea) and powdery mildew (Golovinomyces cichoracearum) than South Asian domesticates.

Provenance and uses​

Herbarium specimens from the 19th-early 20th centuries come from Afghanistan, northwest Pakistan, Turkestan (Uzbekistan, Tajikistan, Kyrgyzstan, Xīnjiāng Region in China), and Iran. These plant were cultivated for sieved hashīsh (nasha, charas) and sometimes for seed oil.

Comments​

Vavilov (1926) characterized afghanica as “a morphological link between the wild and the cultivated races of hemp.” However, evidence in Vavilov and Bukinich (1929) suggests a domesticated phenotype (argued in Suppl. material 1: SF.6). Small and Cronquist (1976) treated afghanica as a domesticate, synonymized under C. sativa subsp. indica var. indica. Small (2018) commented, “The characteristics of indica type marijuana are highly consistent with those of an advanced cultigen. Like modern oilseed cultivars, they are short and compact, an architecture reducing diversion of energy into stem production and increasing harvest index for the desired product (inflorescence). Even the foliage (with very large, wide leaflets) is consistent with the trend described earlier of advanced cultigens often manifesting larger leaves than their wild and more primitive cultivated relatives. When indica type strains are allowed to set seed (they are normally harvested for flowering material) the infructescences are very dense, preventing most of the seeds from falling away and being distributed naturally – another indication of considerable domestication.” The prominent sugar leaves in the inflorescence may be another indication of domestication, as these likely increase photosynthate production very close to the developing flowers and their perigonal bracts.

Figure 5.
Type specimens of C. sativa subsp. indica var. afghanica. Neotype on left (a), epitype on right (b).

 

[acespicoli]

Variety 2: South Asian wild-type​

Diagnosis​

Plants with THC% ≥0.3% in inflorescence and a THC/CBD ratio often ≥7, sometimes less; central leaflet length:width ratio ≥6 in fan leaves near the base of inflorescences; mature achenes usually <3.6 mm long, with a persistent perianth and a protuberant base, and readily disarticulating from plant by a well-developed abscission zone.

Morphology​

Plants 1.0–3.0 m tall. Central stem (stalk) internodes relatively long (often >10 cm, shorter in shorter plants), somewhat hollow (up to 1/2 stem diameter). Branches flexible, diverging from the stalk at relatively acute angles (around 45°).

Leaf palmately compound, larger leaves usually with at least 7 leaflets, leaflet edges not overlapping. Central leaflet long and narrow, lanceolate in shape; margins with moderately coarse serrations, and rare secondary serrations.

Female inflorescence (and infructescence) elongated and somewhat diffuse, with relatively obscure sugar leaves (a high perigonal bract-to-leaf index).

Sugar leaves with CSGTs limited to the proximal half. Perigonal bract covered with a moderate density of CSGTs. Perianth membranous, hyaline with pigmented areas (brown and mottled or marbled in appearance); always persistent.

Achene usually <3.6 mm long, exocarp green-brown; with an elongated base and abscission zone that is relatively narrow.

Phytochemistry​

Dried female inflorescences: THC ≥0.3% (although two studies report plants with THC <0.3%); weighted x¯ = 1.49%, range between 0.06% and 9.3%. THC/CBD ratios vary; two studies (those with THC <0.3%), who shared accessions, reported ratios of only 1.28 and 1.56; these accessions may represent East Asian fiber-type domesticates that reacquired wild-type traits. Ratios in other studies are >10, even >100. THC content and THC/CBD ratios are skewed by THCV%+CBDV%, which is higher than any other variety: x¯ = 0.90% (Hillig and Mahlberg 2004). The terpenoid profile is similar to that of var. indica, except for higher levels of β-myrcene, cis-ocimene, and β-caryophyllene

 

[acespicoli]

Variety 4: Central Asian wild-type​

Diagnosis​

Plants with THC% ≥0.3% in inflorescences and a THC/CBD ratio <7 (almost always >1); central leaflet length:width ratio <6 in fan leaves near the base of inflorescences; mature achenes usually <3.6 mm long, with a persistent perianth and a protuberant base, and readily disarticulating from plant by a well-developed abscission zone.

Morphology​

Plants usually < 1.5 m tall. Central stem (stalk) internodes short (often 5–11 cm, shorter in shorter plants), mostly solid, central hollow, if present, usually less than 20% of stalk diameter. Branches in well-developed plants begin close to ground level, at an angle sometimes nearly 90° from the stalk axis, producing a menorah-shaped habitus.

Leaf palmately compound, dark green, larger leaves with 5–7 leaflets, sometimes overlapping. Central leaflet relatively short and broad, often oblanceolate in shape; margins with coarse serrations, secondary serrations rarely seen.

Female inflorescence small but somewhat compact, with moderately prominent sugar leaves (a moderate perigonal bract-to-leaf index).

Sugar leaves with moderately dense CSGTs on the proximal half. Perigonal bract densely covered with CSGTs. Perianth membranous, with dark brown pigmentation in a mottled or sometimes linear pattern; persistent but easily flaked off with manual manipulation.

Achene small, oval to elongate, exocarp dark olive colored, with an elongated base.

Phytochemistry​

Dried female inflorescences:

THC ≥0.3, literature weighted x¯ = 1.49%, range between 0.4% and 4.47%.

THC/CBD ratio literature weighted x¯ = 2.23%, range 0.77 to 4.75 (one outlier 9.43).

Terpenoid profile likely approximates that of the Central Asian domesticate, but has not been reported in the literature

 

[acespicoli]

Morphological characters​

Approximately 1,100 herbarium specimens were examined, at 15 herbaria, designated by herbarium acronyms in Index Herbariorum (Suppl. material 1: SF.4). Additionally, we extracted morphological data from CGEs that compared Central and South Asian germplasm collected in the previous century (e.g., Vavilov and Bukinich 1929, Small et al. 1976, Anderson 1980, de Meijer 1994, Hillig 2005b). We also drew on morphological data from archaeobotanical studies. In the spirit of open access, extracted morphological data are provided in Suppl. material 1: SF.8, permitting readers to synthesize the raw data for themselves. CGE studies provided data often absent in herbarium specimens, such as plant height, internode length, stalk thickness, and branch angle or divarication.

Branch angle or divarication measured the angle, in degrees, that a branch came off the vertical shoot; it generally ranged between 35° to 85° from vertical. Branch angle may be a function of internode length, which was also assessed. Branch flexibility is a qualitative measure of the ability of a branch to bend or droop without snapping. Flexibility likely reflects the ratio of bast fiber (flexible) to wood fiber (inflexible). Leaf morphology was assessed in “fan leaves” (i.e. larger palmately compound leaves) near the base of inflorescences. The sampled leaves conformed to the concept of 1st order branching off the main shoot, as presented by Spitzer-Rimon et al. (2019). Central leaflet length/width ratio (L/W) is expressed as a quotient. Leaflet shape was either lanceolate (the widest part is less than midway down the length of the leaflet from its base), or oblanceolate (where the widest location is more than half way down the length). This was measured as the distance to the widest point (WP) divided by the entire length (WP/L). A leaflet with WP/L > 0.5 is oblanceolate (Anderson 1980).

The perigonal bract (also called bracteole, perigonium, or inappropriately “calyx”) is the floral bract enclosing the female flower and later the achene (Small 2015). Inflorescence density was qualitatively assessed using the “perigonal bract-to-leaf index” (i.e., the “calyx-to-leaf ratio,” Clarke 1981). Inflorescences with a low index have a predominance of leaf material – interstitial “sugar leaves” (relatively small leaves with few leaflets occurring in the inflorescence) between clusters, subtending 2nd order to 7th order branchlets (Spitzer-Rimon et al. 2019). A low index is associated, in part, with short internode length and broad leaflet width.

The density of capitate-stalked glandular trichomes (CSGTs) was qualitatively assessed (i.e. visually evaluated) on perigonal bracts. CSGT density was mentioned by Christison (1850) in one of the first CGEs that compared C. sativa (Scottish hemp) and C. indica (Indian gunjuh). He noted that C. indica inflorescences felt resinous when touched, “Floral leaves, bracts, and perianth covered with glandular pubescence.” He also noted that C. indica leaves produced “both sessile glands and glandular hairs [CSGTs].” CSGT density on sugar leaves was also qualitatively assessed, based on the method by Potter (2009).

As used here, the “fruit” includes the achene and its more or less adherent perianth. In female flowers of Cannabis, the perianth does not produce a corolla, but instead adheres to the exocarp (outermost layer of the achene wall). Dimensions and appearance of the fruit were assessed.

For each herbarium specimen, a standardized form was used to record specimen label data (collector name, date, location, annotations) and morphological data. During the course of this study, morphological characters were added (e.g., branch angle, inflorescence density, CSGT density), necessitating return visits to some herbaria (BM, ECON, GH, IND, K). Morphological data were synthesized qualitatively (e.g., branch flexibility, leaf color, inflorescence density, CSGT density, perianth adherence), or quantitatively (e.g., plant height, internode length, leaflet L/W and WP/L ratios, achene size). Quantitative data provided bracket measurements for each described taxon.

Phytochemical characters​

A widely-cited paper by Turner et al. (1980) listed 420 phytochemicals isolated from C. sativa – the 420 plant. Few phytochemicals provide useful taxonomic information, however. Our study focused on cannabinoids and terpenoids. In living plants and freshly harvested tissues, cannabinoids exist predominantly in the form of carboxylic acids. THC occurs as tetrahydrocannabinolic acid (THCA); cannabidiol (CBD) occurs as cannabidiolic acid (CBDA). Decarboxylation of the cannabinoids into their neutral counterparts occurs relatively slowly with aging, and rapidly with heat. Thus THCA converts to THC, and CBDA converts to CBD. In addition, when THC ages (unless appropriately stored) it substantially transforms to cannabinol (CBN), an oxidation product. In this paper when THC and CBD are mentioned it should be understood that depending on context, “THC” may mean THCA + THC + CBN, and “CBD” may mean CBDA + CBD.

Rather than cannabinoid quantity (i.e., THC% w/w), we report a parameter measuring cannabinoid quality: the THC/CBD ratio (THC% w/w divided by CBD% w/w). The THC/CBD ratio is a quite conservative (stable) character, whereas THC% correlates with morphology, such as trichome density (Potter 2009), as well as inflorescence density and gland head size. These morphological differences do not alter the THC/CBD ratio. The ratio is determined by a single gene with codominant alleles (de Meijer et al. 2003), or two tightly-linked yet separate THCAS and CBDAS genes (Van Bakel et al. 2011, Laverty et al. 2019). Weiblen et al. (2015) identified a single quantitative trait locus (QTL) associated with the THC/CBD ratio.

In contrast, THC% expression is polygenic, altered by many genes that contribute to morphological differences. Environmental factors (light intensity, temperature, soil nutrients, etc.) alter THC%, but have much less effect on THC/CBD. As a dimensionless ratio, THC/CBD provides a more valid comparison of many studies that grew plants under different conditions (Grassi and McPartland 2017).

Tetrahydrocannabivarin (THCV) and cannabidivarin (CBDV) are short-tailed C19 analogs of THC and CBD. The biosynthetic pathway leading to THCV and CBDV diverges early, on the resorcinol side of the cannabinoid pipeline. Some researchers add C19 analogs to THC/CBD ratios, as THC+THCV/CBD+CBDV (e.g., Turner et al. 1980). Here, the percentage of C19 analogs (THCV%+CBDV%) is treated as a separate character.

Terpenoids constitute the “essential oil” of Cannabis. Terpenoids include hydrocarbon terpenes and their oxygenated derivatives, which form alcohols, ethers, aldehydes, ketones, and esters. They are volatile, and give the plant its characteristic smell. Christison (1850) noted that Indian gunjuh emitted a balsamic odor, lacking in Scottish hemp. South Asian landraces often smell “herbal” or “sweet,” whereas Central Asian landraces give off an acrid or “skunky” aroma (Clarke 1981).

Genetic characters​

Molecular genetic studies of Central and South Asian populations – which have not been significantly hybridized in recent times – are limited in number. Twenty years ago, when unhybridized landraces were much more readily available, molecular methods were blunt instruments. Today, we can decode the DNA sequence of whole genomes, but a good representation of the range of unhybridized biodiversity is not available for analysis, although collection of genuinely representative germplasm from Asia may still be possible. Herbaria of course are invaluable repositories of older specimens, but collections from Asia are relatively limited, and for various reasons, curators have often been unable to allow sampling of older collections.

Herbarium voucher specimens were deposited for some CGE studies (Small and Beckstead 1973; Turner et al. 1973, 1979; de Meijer et al. 1992; de Meijer 1994; Hillig 2004, 2005a; Hillig and Mahlberg 2004; Gilmore et al. 2007), which we examined to ascertain correlations with morphology. For other phytochemical and genetic studies, we relied upon reports of geographic provenance of their accessions.

Results​

The electronic version of this article in Portable Document Format (PDF), in a work with an ISSN or ISBN number, represents a published work according to the ICN (Turland 2018). Hence the new names contained in the electronic publication of this article are effectively published under the ICN from the electronic edition alone. New names contained in this work have been submitted to the International Plant Names Index (IPNI, http://www.ipni.org), from where they will be made available to the Global Names Index.

An example of a taxonomic trait shifting over the past 50 years, as Central Asian landraces hybridized into “Indica”, is provided in Fig. 2. It illustrates a convergence in THC/CBD ratios over the past 50 years. In studies of accessions collected in the 1970s–1990s, Central Asian landraces (study numbers in unitalicized red font), the THC/CBD ratio, expressed as a quotient, was always < 7 (study size weighted mean = 3.56). In studies of South Asian landraces collected in the 1970s–1990s (study numbers in italicized green font), the THC/CBD ratio was ≥ 7 (study size weighted mean = 97.14). Since then, THC/CBD ratios have skyrocketed in accessions purportedly representing Central Asia (i.e., “Indica”). Now there is little or no difference between “Indica” and “Sativa”.


Figure 2.
Shifts in THC/CBD ratios over time; data from 47 numbered studies in Suppl. material 1: SF.9. Central Asian landraces in unitalicized red (n =13 studies); “Indica” in underlined unitalicized red (n= 9); South Asian landraces in italicized green (n =18 studies); “Sativa” in underlined italicized green (n =7 studies). Size of numeral reflects the number of accessions analyzed in that study.

Taxonomic analysis​

We classified C. sativa subsp. indica into four varieties (in the formal nomenclatural sense, i.e., varietas). Two varieties express traits of domestication (identical to “Indica” and “Sativa” in the original narrow meanings of these terms), and two varieties have wild-type traits. We followed precedent set by Small and Cronquist (1976) who segregated C. sativa subsp. indica into two varieties – domesticated and wild-type plants. They did not place these varieties in an ancestor–progeny relationship, however, because they could not verify putative ancestral relationships.

 

[acespicoli]

I gave the 79xmas seeds to ndnguy.
Subcool didn't have anything to do with them either.

Had a nice lot of seeds late 80s the buds smell like straight xmas trees
They looked like someone took copper strand wire and balled it up and smashed it flat that many copper hairs
Really good stony smoke as well, laid a few people down for a nap
I would take breaks between smoking it pretty potent for the old days.
Hopefully its the same as I remember been thinking about giving it a go.

'79 Xmas Bud IBL - Humboldt CSI

1979 Xmas Bud IBL Open pollinated reproduction of the pure […]

humboldtcsi.com

Got this as well as some others as freebies when I placed a couple orders last year. I messed up and let them get too cool as young seedlings, so they got scrapped for the outdoor project last year. Not sure if I have any left. I need to look!

Running on stoner time cant complain cause I do the same ....
Have a few things in the pipe ahead of this so its a interest have to see how things play out
Already have some PTK not sure how they compare but orig just trying to get back to xmas tree bud
They were definitely unique, think ole dude I got them from was growing them but out of respect didnt ask...
Wish I would have saved seeds from quite a few strains over the decades,...

 

[dogzter]

Had a nice lot of seeds late 80s the buds smell like straight xmas trees
They looked like someone took copper strand wire and balled it up and smashed it flat that many copper hairs
Really good stony smoke as well, laid a few people down for a nap
I would take breaks between smoking it pretty potent for the old days.
Hopefully its the same as I remember been thinking about giving it a go.



Running on stoner time cant complain cause I do the same ....
Have a few things in the pipe ahead of this so its a interest have to see how things play out
Already have some PTK not sure how they compare but orig just trying to get back to xmas tree bud
They were definitely unique, think ole dude I got them from was growing them but out of respect didnt ask...
Wish I would have saved seeds from quite a few strains over the decades,...

I would be curious to see what they have become since then myself.

 

[acespicoli]

Small and Cronquist further classified the two subspecies into four distinct varieties, namely;

• Cannabis sativa subsp. sativa var. sativa (hemp variety with domesticated characteristics);
• Cannabis sativa subsp. sativa var. spontanea (hemp variety with wild characteristics);
• Cannabis sativa subsp. indica var. indica (marijuana variety with domesticated characteristics);
• Cannabis sativa subsp. indica var. kafiristanica (marijuana variety with wild characteristics).

 

[acespicoli]

Origin and Evolution of the Cannabinoid Oxidocyclase Gene Family - PMC

Cannabis is an ancient crop representing a rapidly increasing legal market, especially for medicinal purposes. Medicinal and psychoactive effects of Cannabis rely on specific terpenophenolic ligands named cannabinoids. Recent whole-genome sequencing ...

www.ncbi.nlm.nih.gov

Trema orientale - Wikipedia

en.wikipedia.org

Phytochemicals​

Trema orientalis has been reported to contain cannabinoids such as tetrahydrocannabinol (30mg/kg-90mg/kg avg), cannabinol (130mg/kg-357mg/kg avg) and cannabidiol (2mg/kg-5mg/kg avg).

Medicinal use​

The tree has various uses as an herbal medicine in a wide range of cultures.[7] The leaves and the bark are used to treat coughs, sore throats, asthma, bronchitis, gonorrhea, yellow fever, toothache, and as an antidote to general poisoning.[11][14] A bark infusion is reportedly drunk to control dysentery and a leaf decoction is used to deworm dogs.[11] In recent pharmacological studies, an aqueous extract from the bark has been shown to reduce blood sugar levels in an experimental animal model of diabetes mellitus, and may be useful for treating this disease.[15] Extracts from leaves of related species (Trema guineense and Trema micrantha) showed anti-inflammatory, anti-arthritic and analgesic activity in rodents,[16] suggesting that T. orientale could produce similar results.

 

[acespicoli]

@TM Colombian

I just read all the posts from my last post #1945 to #2376.The seeds I sold to Nevil were all made by me mostly from Holland work.
I never sold any I collected from Haze I obtained from the Haze Bros. I sold my work.
J & G are the same guy. Almost all my OHaze seeds were from him.
I never sold any OHaze X S Indian Kerala or Thai as OHaze, I always called them OHaze X whatever.
Burning Bush was 100% pure OHaze I grew it in Calif and named it, RCC also smoked it with me, and loved it. That and another OHaze I called Cream De La Cream and another I called Mr Greasy even though it was a girl, it had very very streched laddery buds that even when dry and placed in a zip bag greased up the bag unbelievably, it was also hard to roll and keep a joint lit.
Nevil did not have OHaze seeds from before the 70's 80"s as he got his OHaze from me and I only sold him seeds I had made, all 100% OHaze, but not from the 60's or early 70's as I did not start making OHaze seeds until the mid 70's and in large amounts in the 80's in Holland. I think I sold him seeds I made in Holland after 85'.
I will try and answer a few questions if I know the answers, but will ignore any questions I have answered before. Dont ask me about others Haze as I was not the breeder you need to ask the breeder.
The reason that OHaze was seldom found in Coffee shops in Amsterdam is because commercial growers wanted herb with a short flowering period, why would they grow a OHaze that took 16-24 weeks and did not yield as much as a 7-8 week plant?
That and I was a seed producer not a commercial grower if I was I would have worked up an OHaze pure, that was better then NH at least to me.
I get that not all liked OHaze X Skunk or Thai/OHazeXSkunk but I did and many others I know also did.
I also have yet to find any Cannabis that had a more Cerebral, Clear, Up, Psychdelic high with no celing that got you higher every time you took a toke. Nevils did not, for me anyway.
People have many different preferences I also have mine. RCC agrees with me if that matters. I did not see OHaze until the very early 70's I did see both G's and RL's my wife manicured for G and he was the first OHaze Bro, we were close back in the day, and his was the best. Shame he quite growing OHaze but it was so much easier to get my Skunk #1 seeds for his grows, this was before people maintained clones, and his OHaze was already suffering from inbreeding depression, I did not try and improve my OHaze seed lines I tried to preserve all the genes and only sold them with the advice to use them for breeding not commercial growing, to use for commercial growing you need to grow thousands and select the best 10 clones to reproduce for product, few people wanted to do that.
I sold Nevil seeds for $1 a pop for any of my varieties.
I sold by the kilo for €5,000 there are 50,000 - 75,000 for small seeds like OHaze.
I still have a KG of OHaze seeds made a decade ago they are not for sale they are for a OHaze project I am trying to arrange outdoors in the ground at a latitude of 18 just to find and clone the best 10-20 for production where legal.
I have zero problems to keep well made seeds alive stored under refridgerated 4c for 25 years so they are still germainating in the high 90's if and when I do this I will post photos of the 10-20 as well as their Cannabinoid and terpene profiles.
These are slightly over 75 per gram so 75,000 seeds and normally I get 70% females with OHaze so I will have lots to select the 10-20 keepers from, I expect about 50,000 females if started carefully. I am retired from selling seeds, but I will allow others to do what they want with my work, they will pay me for the work, and it is not work as I want to do it even retired.
FYI the OHaze grown by the Haze Bros were not all equal in quality, maybe 10% were fantastic, 50% good or great, and maybe 10% not as good but most would still think it was great. Each year after 1970 the quality and yields and vigor declined a bit. By 1980 I did not know any large OHaze growers, for the reasons I have stated.
I have told the truth, I have zero to gain by altering the truth, I am retired for several years now. I have had a great life, few complaints, I would like to put out a dependable pure OHaze seed line or two for my self and others, even if I do not sell them but let others do so. Selling is work, I do not need the $ and I will only do what I love today. Breeding OHaze is not work for me it is fun, and I think I can do it better then anyone else just because of my experience and history.
FYI I have all but given up on Phylos, they have other goals then my interest in Cannabis Evolution and Relationships, but FYI I only sent them extracted DNA no living materials they could steal, I also sent them many of my varieties DNA, focused on Landraces to help explore my interest in Evolution and Relationships. I am glad I tried, nothing ventured nothing gained.....
-SamS

 

[acespicoli]

 

[acespicoli]

When I asked Skunkman Sam how he was able to breed Skunk #1 to be relatively true breeding,
he very humbly said,

“I didn’t, nature did.
I just had to cross together and test enough plants until I found the combination that worked.”

 

[acespicoli]

The NL hybrids were created in the early 80s by a group of friends around a former Marine and Vietnam veteran nicknamed Seattle Greg. Greg told me that in 1979 he received just 4 seeds of the “Purest Indica” from the author Murphy Stevens, who published one of the most advanced cultivation books in the mid-70s titled: How to Grow Marijuana Indoors Under Lights.


Greg crossed these four seeds together as an IBL or “in-bred line” and passed the seeds that he made out to his friends who then out-bred the “Purest Indica” with the varieties that they were already growing. They shared some of their seed creations back with Greg, who grew them out and then numbered them from #1 to #11 based on #1 being the closest morphology to the “Purest Indica” and #11 being mostly tropical or narrow-leaf. The ever popular Northern Lights #5 was in the middle with a nice balanced combination of both the “Purest Indica” and equatorial genetics.

The Primary Colors of Cannabis

The first modern cannabis hybrid was created in the summer of 1969 in the Santa Cruz mountains by a surfer we only know by his first initial “G”, and legend has it that he crossed together three varieties from Colombia and created what the world has come to know as Original Haze.

hightimes.com

 

[acespicoli]

This strain is is an IBL line of a 1988 G13-Hashplant
(Aka: Mr.Nice), mostly done by NDNguy and friends with the wishes that no one profit from the pure seedline.

• Mr. Nice G13 x Hash Plant
  • G13 Hash Plant
    • »»» G-13 x Hash Plant F1
    • Hash Plant
      • »»» Hash Plant x {Hash Plant x NL #1}

Purest Indica

The Seed Banks Northern Lights #1 Description​

NL #1 is a true-breeding Afghani, with extreme Indica characteristics. These are short, stocky plants, with leathery, dark green, extremely broad leaves. The stems ere very strong, and it is easy to clone. As the seedlings generally tend to grow to one main stem, this is an excellent choice for the "Sea of Green" or "Small Plant" method. Resinous, potent, and sweet, not nasty and acrid like some Afghanis. Very vigorous end cold resistant, works well outdoors with a long season.

Indoor height at 100 days: 40-50 inches.
Indoor yield at 100 days: up to 1000 grams.
Flowering period at 12 hours of darkness: 50-55 days.
Outdoor height: 5-7 ft.
Outdoor yield: 1,5-2 Ibs.
Outdoor finishing date: October 15 at 45° N. latitude.

 

[acespicoli]

billye California Orange

Tangie »»» California Orange x {Skunk x Unknown Strain}

• Crockett's Tangie »»» Cali-O x Skunk

 

[acespicoli]

Plants 2023, 12, 3927. https://doi.org/10.3390/plants12233927

1:200