Cannabis grafting

[gorilla ganja]

Awesome work Darpa, nice to see some solid lab work and testing. Respect

I agree. Very interesting experiments you have going on here.
Looking forward to seeing how things play out.


Peace GG

 

[Darpa]

I agree. Very interesting experiments you have going on here.
Looking forward to seeing how things play out.


Peace GG

Thanks Gorilla Ganja!

Darpa

 

[Darpa]

I would be interested in doing some experiment on arginate coated artificial seed….

May be a futur experiment…



Anybody interested?


Darpa

 

[gorilla ganja]

Yes, please.
That would be interesting indeed.

 

[Darpa]

Has anyone tried “cold fusion” yet? What I mean is grafting to an unrooted cutting and letting the graft heal while the clone roots.

Hi Hookahhead, I'm testing cold fusion at this moment and preliminary result are excellent. But to add a twist, I used 25 days old scions stored at 4 degree and graft them on unrooted cutting…

Hope this will answer your question.

Darpa

 

[Florere]

Fuck yeah I’m interested..


2 silly questions.

What you mean with 3x with your washes?

Like: soap-rinse-bleach-rinse and that’s one so two more times or
Soap-rinse-soap-rinse-soap-rinse that’s 3 and than the bleach-rinse 3x?

And what you mean with 10% bleach ?

Thanks

 

[Darpa]

Fuck yeah I’m interested..


2 silly questions.

What you mean with 3x with your washes?

Like: soap-rinse-bleach-rinse and that’s one so two more times or
Soap-rinse-soap-rinse-soap-rinse that’s 3 and than the bleach-rinse 3x?

And what you mean with 10% bleach ?

Thanks

Hi Florere,
the 3x with my wash mean Soap-rinse-soap-rinse-soap-rinse (for about 10 min) and then the bleach-rinse 3x for an other 10 minutes total.

I use comercial bleach that contain 12% sodium hypochlorite. I make a dilution of the comercial bleach solution to 1,2% sodium hypochlorite.

Ex: 100 ml of comercial bleach + 900 ml water

https://www.sigmaaldrich.com/technical-documents/protocols/biology/explant-sterilization.html


Darpa

 

[JustSumTomatoes]

Wow, yeah I'd love to see that. I love these types of experiments and research. Pushing the boundries of the plant and making discoveries along the way.

 

[Darpa]

Hi Florere,
the 3x with my wash mean Soap-rinse-soap-rinse-soap-rinse (for about 10 min) and then the bleach-rinse 3x for an other 10 minutes total.

I use comercial bleach that contain 12% sodium hypochlorite. I make a dilution of the comercial bleach solution to 1,2% sodium hypochlorite.

Ex: 100 ml of comercial bleach + 900 ml water

https://www.sigmaaldrich.com/technical-documents/protocols/biology/explant-sterilization.html


Darpa

The desinfection - sterilisation of initial plant material is a crucial step since I've been working with heavely contaminated plant:









Last year I got my main grow room contaminated with aphnid, and I had to incorporate ladybugs to control the contamination. This was verry effective. However, this year, to avoid this problem, I use 3 different grow area (contaminated zone- decontamination zone and a clean zone).

Darpa

 

[GMT]

Hmm I've tried throwing a lady bird into the grow, but it just flew up into the HP's bulb and then fell back again crispy. How do you stop it raining dead lady birds in there?

 

[Darpa]

Hi IC Friends,

I deceided to test a different preservation method for the sub-zero cold storage of plant material.

This time sterilized scions were directly immerged in the CPA solution (30% glycerol) and were stored right away in the freezer without any acclimatation in the fridge….

[URL=https://www.icmag.com/ic/picture.php?albumid=78479&pictureid=1999607&thumb=1]View Image[/url]

[URL=https://www.icmag.com/ic/picture.php?albumid=78479&pictureid=1999608&thumb=1]View Image[/url]

We will see if this could be an alternate method for the cold storage of plant material for futur grafting experiment…


Darpa

A concentration of 30% glycerol in the CPA solution seem to work
just fine. The CPA soaked paper used to wrap the scions do not freeze at -20 celcius and the immerged scions do not show any sign of ice formation…






Darpa

 

[zif]

A concentration of 30% glycerol in the CPA solution seem to work
just fine.

Is there a step to rehydrate / wash the glycerol from the tissue prior to using the scions?

If you can just run with these, that's about as straightforward as cold storage could possibly be!

 

[Darpa]

Is there a step to rehydrate / wash the glycerol from the tissue prior to using the scions?

If you can just run with these, that's about as straightforward as cold storage could possibly be!

Hi Zif,
I think I'll try a 24h acclimatation at 4 degree then a cold water wash…

We will see how it turn out…


Darpa

 

[Darpa]

Update of the 3 seeding graft:

On the 3 seedling graft, one of them is a male and the other is a female, the third one in unknown at this time. If you guy remember, I made seeds croosing the BikerKush x (Original Glue4(GSC x TrainWreck) with one of the male scion of FaceOffxWTC grafted on the same plant. (post 322)

The FaceOffxWTC have a high trichome count even in vegging (see post 256) and it seem that it transfert this trait to the (FaceOff x WTC) x (BikerKush x (Original Glue4 x (GSC x TrainWreck)))

Here a some macro pic of the male branches:









Leaf:




Darpa

 

[Darpa]

Hi IC friend!

Here a some update!

A last marco photo of the (FaceOff x WTC) x (BikerKush x (Original Glue4 x (GSC x TrainWreck))) male:



I know about trichome types and expression - differentiation, but I'm wondering if some of the breeder out there have used Male Plant Trichome Density as one of their selecting tool... This would be interesting in a BX project....

Extream trichome density male (EDM) cross with high glandular tricome female. - Rating the high trichome density trait expression (1) (m#f) - Selecting High density female from (1) and crossing it back with the initial male EDM...Just thinking....

Ok UPDATE ON COLD STORAGE OF CANNABIS SCIONS FOR A GRAFTIN PORPOSE experiment (post 339 +)



Waxed tip scions stored at 4 degree for up to 35 days are still healthy and woud be accetable for grafting...(post 340)

I changed the paper towel and stored it back at 4 degree.




UPDATE ON THE GRAFTED 25 DAYS COLD STORAGE SCIONS ON UNROOTED ROOTSTOCK:

Plant look pretty good in general. I should have put the clones in a humidy dome instead of in mason jar... I got some fungus contamination, probably comming from the unsterilized clone rootstock plant material.,, and it affected few of the scions...



Some grafted clones already show nice roots proliferation as the graft is healing!



Cheer

Darpa

 

[Darpa]

Hey folk!

If some of you are interested in experimenting Cold Storage (fridge - freezer) trials...

Jump in!

I would be very happy to see how long we can preserve plant material (wich is not calculate in the plant count number) for grafting or grafting purpose at home....

Darpa

 

[hellfire]

Multi strain grafted moms with air layering to take clones...good way to keep numbers down

 

[Pédetoddy]

I did some graftings of frisian duck sicions in purple haze x malawi stock.
Mother plant

Graftings
https://www.icmag.com/ic/picture.php?albumid=82112&pictureid=2002453

 

[Pédetoddy]

 

[Darpa]

View Image

Thanks Pédetoddy for sharing!!!

I would put those grafts in a humidity dome asap if you don't want the scion to dehydrate and die,,,,

Cheer


Darpa