16,000w Coco 6/9 DTW tree grow

[Imationkarl]

rh that high? i usually run around 45%.

 

[Miraculous Meds]

rh that high? i usually run around 45%.

That is certainly safer in flower, but after reading posts here from folks like dhf, etc.. that 70% in veg is what they love, and 50% in flower, but I get away with mid 60% by having massive air exchange and good air circulation.

 

[Imationkarl]

i have amazing air circulation. that 13'x20' room has three 18" hurricane oscillating fans. i would love to be able to bump the RH to 60... i might try and get away with 55 with co2. gonna turn the burner on tonight running 1000ppm, 85 degrees, and 55%rh and see if everything can handle it.

and yeah, i agree on high rh in veg. my room is about 40% and i'm thinking about adding a humidifier.

 

[Imationkarl]

to everyone asking about the cell culture:

i asked the scientist running that project to come to this thread and answer some questions. please welcome him.

 

[Lesterburnum]

Subbed up and welcome tissue culturer scientist. Good looking gig man!. Would love to see some more pics.

 

[SecondAttempt]

Sweet setup... let's hear from the scientist.

 

[theother]

It amazes me that u invest all this money and work, and haven't installed ur filters yet. Get on that shit, it would have been better if they never smelled it.

Your not wrong, better late then never but just the same, would have been worthwhile ahead of time. .

 

[Pharmacologí]

Tissue Culture

Tissue Culture

Hello,

I'd have a few more pictures, but uploading pictures to this forum with only an iPad has proven difficult to a humorously notorious level.

Pharmacologí uses tissue culture to provide genetic banking services and clone production at any level, including mass production.

Below is shown the one photo that's managed to upload properly. A single axillary bud from a tertiary shoot of a mother plant has produced an in vitro shoot from which many clones will be proliferated.

Karl and I will be working with Gravedigger Seeds to store their genetics and produce clones to order.

 

[Pharmacologí]

Here is callus tissue of Snocap x LA Confidential, a superb robust cultivar. Callus which is snowy or opaque white is superior in producing plant organs, and the cultivar's vigor is easily seen in vitro. Callus tissue is wound tissue which has meristematic clusters. These clusters produce shoots from which many clones can be produced.

Mother callus tissue is my deepest, most long term form of tissue storage. This ensures I never lose a client's genetics. Another benefit is that as long as your clones are being produced in vitro from mother tissue in this fashion, your prized cultivar will not weaken or senesce over time as with traditional cuttings because of the extensive cell proliferation (growing plants out from plantlet to mother many many times and taking cuttings from each successive generation).

 

[Snype]

Nice work Pharmacologí! So can you store these things for years until you need to get a cutting out of them? If so how does one have to store it? Pretty amazing! I'd love to have these types of options.

 

[Pharmacologí]

Callus tissue proliferates slowly and stores indefinitely in the dark at 75 degrees F. All my callus cultures are subcultured or recultured every six months to ensure fresh nutrient and water filled media is present in the test tube and there they sit until I pull some out from which to propagate plantlets.

The advantage of off site storage with us is that the grower can go on vacation or sit on cultivars they are not interested in growing for the time being but want to retain for their collection and grow out at some later date.

 

["HEMPEROR"]

wow wot a thread just read from the begining upto here and all,ready learned loads of new stuff im subbed and watching in amazement! top work bro..hemp!

 

[Snype]

Callus tissue proliferates slowly and stores indefinitely in the dark at 75 degrees F. All my callus cultures are subcultured or recultured every six months to ensure fresh nutrient and water filled media is present in the test tube and there they sit until I pull some out from which to propagate plantlets.

The advantage of off site storage with us is that the grower can go on vacation or sit on cultivars they are not interested in growing for the time being but want to retain for their collection and grow out at some later date.

Amazing! Is this something that the home grower can do as well or is it a very difficult process?

 

[watts]

Place a vaportek optimum 4000 on the outside of your grow rooms or wherever the smell is leaking. Run it 24/7, perfectly safe.

 

[Imationkarl]

I was being silly and running my filters in the hallway outside of a mostly sealed room. The smell eventually leaked out in massive, massive amounts after the 2nd/last bloom boost around week 6. I moved the filter into the room and it's keeping it down now.

I would like to do a pic update of the 3 rooms but it takes me forever to get pics on here. Using a PC/iphone/ipad. Does anybody have a quick solution to this?

 

[watts]

imgur is a good site to upload pics

 

[Weeded1s]

I just go under advanced click on the paper clip and upload from there . This is all on my phone so I assume its the same. Takes like 5min to upload 5 pics..just let it sit and it s all done. Kinda slow ya..but I just walk away smoke a cig and when I come back its done.

 

[caredrivensf]

Your pumps go off for 15 mins at a time. How many emitters per pot and approx how many liters are you giving em each feeding?? Just wondering cuz I do DTW COCO too!! but not 1 plant per light

 

[stoned40yrs]

Amazing! Is this something that the home grower can do as well or is it a very difficult process?

Did you miss this part of what he said?

"The advantage of off site storage with us"

I'm pretty sure that means he will store your genetics for $$$ and that he isn't going to give out his proprietary information to Joe Blow Grower who will then have less need for his service. Just a wild gue$$ on my part

 

[Weeded1s]

Lets not act like their aint hydro store that sell cultivator kits ..for I dunno maybe 195 out the door. This is a technology that allows people to store genetics at home with out roots popping so they dont count as plant #s. Welcome to science and 1 more way 40 roesnt have to burn shit in his oven at times