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FAMILY Jewels V.2
- Thread starter cocktail frank
- Start date
Everything's lookin great fellas, rep all the way around!
Been a minute since ive been on as well, always workin behind the scenes tho.
Took down a Sour Strawberry Kush today, figured id treat yaz!
i usually do whole plant shots but i didn't this time....
Just grabbed up GSC and popped (5) green crack x pre 98 and (5) Cannabiogen Peyote Purple.
im bringin bubba back, yeah
- SubN
Been a minute since ive been on as well, always workin behind the scenes tho.
Took down a Sour Strawberry Kush today, figured id treat yaz!
i usually do whole plant shots but i didn't this time....
Just grabbed up GSC and popped (5) green crack x pre 98 and (5) Cannabiogen Peyote Purple.
im bringin bubba back, yeah
- SubN
Very nice looking plant...good job!
dank.Frank
dank.Frank
Love this thread! What exactly is the "family"?
-gil
-gil
You'll have to do alot of catching up, but this is a good place to start:
https://www.icmag.com/ic/showthread.php?t=86597
Thank you sir! I'll read through it when I have some time. So I guess it's an east coast thing? Well,I love what you guys do, so I'm gonna pretend I'm like a second cousin or something!
-gil
-gil
Accessndx - do you have a thread chronicling you tissue culture experiments? It would be awesome if was in the realm of practicality. Much better than having cuttings on top of cuttings while hunting for keepers. I looked into it a while back, but I couldn't really find anyone that was having much luck with it. At least not in a practical and useful way. Maybe it's gotten easier/better? I used to grow quite a few mushrooms, so sterile practices are not a problem. What I really want to know is: how hard is it to go from a tissue culture in a jar, to an actual plant?
-gil
-gil
No thread yet. Just playing with tissue culture myself. Some success, but it requires a bit more to dial it in. For example: certain strains just never took off in tissue culture while others seemed to thrive. My G13xBurmese Kush seemed to take immediately to it with vigor, whilst strains like Marley's Collie would look good for awhile, but would die upon transfer to another container.
There are some reasons I suspect this happened:
1) I changed tissue culture formulations midstream.
2) I didn't have my pH dialed into the media properly.
3) Temperature changes affect tissue culture profoundly, hence the summer is a tough time.
4) I tried to get too many strains into culture at one time.
I'm looking for a slightly better tissue culture media right now. The TDZ formulation works well, but there has to be one more ideally suited for cannabis. I have to look specifically at the types of hormones used as well as nutrients available. There hasn't been too much published on the subject, but come winter I'm going to try to mix up a batch of something that combines the same nutrients I use in hydroponics.
All things being equal, the amount of space that the cultures require is obviously small.....BUT there's a tremendous amount of upkeep.
You're moving stuff every 2-6 weeks and it's alot of juggle. A key change would be to enable the cultures to remain in a sort of stasis for a longer duration without lots of development that require transfer. Some of this can be accomplished by dropping the temperature or via refrigeration. Larger containers would also be valuable because they contain more media, therefore more food and have to be swapped out less.
Anyway, I'm not done by any stretch....just doing a bit more bookwork on the subject to prepare for better experiments.
All my craziness will be documented here of course.
Oh, you wanted to know how difficult it is to go from a jar back to a plant eh? Well it's fairly easy in some circumstances because once an explant utilizes all the hormones available to keep it from rooting, it begins to root automatically. It can then be transferred into an ezcloner for hardening and possibly even soil immediately. I had a tissue culture grow a full rootball in culture just by keeping it in the same container for a bit longer.
Glad to hear you say that, $! 
dank.Frank
dank.Frank
simple green
Member
Give me my $$$$$$$$$$$$$$$$$ back!
^^^ Yeah, that too!
dank.Frank
dank.Frank
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(DaywreckerD x OG) x OG 
41.days 12/12
41.days 12/12
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