Dud Identification Collective Knowledge.

[mofeta]

Here are a couple more papers on phytoplasma in Cannabis. Sorry if they have already been posted.

‘Candidatus Phytoplasma asteris’ (group 16SrI) associated with a witches’-broom disease of Cannabis sativa in India


WITCHE'S BROOM AND PHYLLODY LIKE SYMPTOMS OF DISEASES IN Acalypha indica L. AND
Cannabis sativa L. -A NEW REPORT FROM CHAMPARAN, NORTH BIHAR

Check out this awesome pic from the second one, wow that is Witches' Broom like a big dog!

 

[mofeta]

Here is maybe the world's leading authority on all aspects of phytoplasma and other MLOs. He is a USDA molecular plant pathologist, and is the very top of the heap in his profession.

He is co-author on that paper about the first recorded phytoplasma in Cannabis in China. His CV is very impressive, check out the honors and awards he has won, even one from the President of the US! Anyway, look at his homepage and publications, I couldn't even dream up someone that would be more interested or capable of investigating this particular kind of dud we are highlighting here. When I look at his publications, it seems like phytoplasma is on the rise in many genera worldwide. I am certain that he would be real interested in this dud type. Imagine the easy, flashy paper: "First Observation and Molecular Identification of Phytoplasma Infecting US Cannabis Crops" wow I bet he'd be chomping at the bit.

I bet he could get the proper DEA permits to do the work very easily.


It should be someone with credentials that approaches him, like say Rob Clarke. Good descriptions, with good pics would have to be included in the presentation so he didn't think he was dealing with a bunch of yahoos. I bet if Rob sent him an email with the info and pics, he would jump on it and we could hook him up with some samples.

Robert E. Davis

 

[RetroGrow]

A number of people on other sites have had their "duds" assayed, and they came out positive for TMV. This would explain the passage of pathogen through seeds. Seems to be rampant in Colorado. So it seems that one of the causes of "dudding" is TMV.

 

[mofeta]

A number of people on other sites have had their "duds" assayed, and they came out positive for TMV. This would explain the passage of pathogen through seeds. Seems to be rampant in Colorado. So it seems that one of the causes of "dudding" is TMV.

Wow thats interesting. I can see where a rosette virus similar to the Rose Rosette Virus could be causing duds alright.

The old saying "correlation is not causation" comes to mind though, I would like to know more.

Unless they used a top pathology lab at a research institution I doubt they screened for phytoplasma. A plant could have both a virus and the MLO.

It could also be the case that phytoplasma and a marijuana rosette virus have very similar symptoms.

I am really becoming curious about the phytoplasma now, it is such an interesting organism. I hope some enterprising person has the time to investigate this properly so I can know what is going on with that.

 

[RetroGrow]

This is the lab:
http://www.allcropsolutions.com/

There is a thread here about TMV, but most people who thought they had TMV really had broad mites. TMV assays were coming up negative. Now they are showing positives, especially on Kush strains, OG Kush & Bubba Kush, also WiFi#9, and White X Fire 06.
They do test for phytoplasmas, but it hasn't been done yet as far as I know. Someone needs to send in some samples of "dudded" plants.

Another lab is here:
http://csplabs.com/cat_detail.php?catID1=Plant Health

 

[Sam_Skunkman]

I would be careful about presuming that TMV was all they had, you can get BM, CM, RM, TMV, and ‘Candidatus Phytoplasma asteris’ (group 16SrI) associated with a witches’-broom disease all in the same plant. As well as Fungal pathogens and other viruses. Oh and nematodes... Or any combo of the above.
Don't count on Rob Clarke to help he is busy with his fishing and I doubt he has the time, or access to computers in Malaysia, out in the country up a river, where he fishes.
I am not in the states, you need to find people that can do this that are in the state he is in so sample plants can be delivered. His phone number is in the Silver Springs MD area. I did try emailing him.
RetroGrow,
Do the two labs you posted test Cannabis plants? Both are in Calif., one in Davis the other in Pleasant Grove. I e-mailed them to ask if they test Cannabis plants.
-SamS

 

[Storm Shadow]

http://www.oregin.info/stakeholders/meetings/shf07-Nov2009/Hogenhout_JIC_OREGIN_SHF7_Nov2009.pdf

 

[Storm Shadow]

http://download.springer.com/static...286_6e9e60784ce184a055157bb9a07f712e&ext=.pdf

Exploring the phytoplasmas, plant pathogenic bacteria

 

[Storm Shadow]

http://www.a.u-tokyo.ac.jp/english/topics/2014/20140318-3.html

How pathogen transforms “flower” into “leaf”? -- Phytoplasma protein degrades floral transcription factors


Phytoplasmas are plant pathogenic bacteria associated with devastating damage to over 1000 plant species worldwide. Infected plants show a wide range of symptoms. Especially, plants exhibiting phyllody symptoms (formation of leaf-like tissues instead of flowers) have attracted much attention, and used to be traded with high commercial value. However, the molecular mechanisms of phyllody symptom development remain to be elucidated.

A research team headed by Professor Shigetou Namba in the Graduate School of Agricultural and Life Sciences at the University of Tokyo identified a novel gene family encoding for a phytoplasma-secreted protein “PHYLLOGEN”, and demonstrated that this protein is a pathogenic factor responsible for phyllody symptoms. His team found that PHYLLOGEN interacts with and degrades floral homeotic MADS-domain transcription factors that are conserved among flowering plants. This degradation of the MADS-domain transcription factors was dependent on the ubiquitin-proteasome pathway. The expression of floral development genes downstream of these transcription factors was actually disrupted in PHYLLOGEN-expressing transgenic plants. Moreover, PHYLLOGEN was genetically and functionally conserved among phytoplasmas. From these discoveries, Professor Namba’s team revealed the molecular mechanisms of phyllody symptom development for the first time.

PHYLLOGEN is expected to induce phyllody symptoms on many plants, as do phytoplasmas. Therefore, PHYLLOGEN will open a new frontier in breeding of unprecedented ornamental plants with green flowers of high commercial value. Moreover, PHYLLOGEN will be useful for increasing forage yield because it keeps plants in the vegetative phase. Development of inhibitors of PHYLLOGEN will also be helpful in the control of phytoplasma diseases.

 

[RetroGrow]

I would be careful about presuming that TMV was all they had, you can get BM, CM, RM, TMV, and ‘Candidatus Phytoplasma asteris’ (group 16SrI) associated with a witches’-broom disease all in the same plant. As well as Fungal pathogens and other viruses. Oh and nematodes... Or any combo of the above.
Don't count on Rob Clarke to help he is busy with his fishing and I doubt he has the time, or access to computers in Malaysia, out in the country up a river, where he fishes.
I am not in the states, you need to find people that can do this that are in the state he is in so sample plants can be delivered. His phone number is in the Silver Springs MD area. I did try emailing him.
RetroGrow,
Do the two labs you posted test Cannabis plants? Both are in Calif., one in Davis the other in Pleasant Grove. I e-mailed them to ask if they test Cannabis plants.
-SamS

One does for sure:

The other one, I am guessing they do, but a phone call would settle that. Stems can be submitted to any lab and called "tomato" stems.
It seems apparent that multiple issues are going on, causing what people are calling "duds".
Phytoplasma: possible, but unconfirmed by lab. A number of people are having assays done now. Still waiting for results.
TMV definitely confirmed by lab.
Fusarium? Definitely confirmed by lab.
Weaponized fusarium?
The first Agent Green fungus is Fusarium oxysporum, a family of “wilting” fungi whose species attack everything from corn and cotton to basil to watermelon. The anti-coca fungus species, Fusarium Oxysporum f. sp. Erythroxyli, was discovered accidentally when it wiped out a test plot of coca being grown in Hawaii. From that strain, the US Department of Agriculture (USDA) developed the strain “Isolate EN-4,” which is supposed to attack only the coca plant, and which the US and UN want to spray extensively in Colombia.

The use of fusarium oxysporum to eradicate marijuana in Florida was proposed in 1999, and was soundly rejected by state officials as being a threat to the environment and agriculture. Secretary of the Florida Department of Environmental Protection David Struhs wrote in an April 6, 1999 letter:

Fusarium species are capable of evolving rapidly... Mutagenicity is by far the most disturbing factor in attempting to use a Fusarium species as a bioherbicide. It is difficult, if not impossible, to control the spread of Fusarium species. The mutated fungi can cause disease in a large number of crops, including tomatoes, peppers, flowers, corn and vines, and are normally considered a threat to farmers as a pest, rather than as a pesticide. Fusarium species are more active in warm soils and can stay resident in the soil for years.
Despite the Florida rejection, the US and UN went ahead with plans to pressure the Colombian government to use the fungus. The Clinton administration at one point tried to tie a $1.3 billion aid package to Colombian government approval of the fungus, and only backed off under considerable international pressure.

In October 2000, the Colombian government formally rejected this portion of the US/UN “Plan Colombia,” which also includes ongoing widespread spraying of glyphosate, the active ingredient in Monsanto’s “Roundup.” Colombian Environment Minister Juan Mayr said, “The government consulted national experts on the subject and decided not to agree to test the fungus because it felt that any agent foreign to the country’s native ecosystem could pose a serious risk to the environment and to human health.” Colombia’s neighbors, Peru and Ecuador, have also expressed concern about the proposed use of the fungus causing them ecological and agricultural damage.

The Colombian government’s position does not make this a closed issue, though. Companies such as Ag/Bio Con Inc., which holds a patent on the process of attaching the fungus to seeds for aerial dispersal, may be expected to keep the issue alive. Ag/Bio Con Inc. is headed by Dr. David Sands who worked on fusarium oxysporum at Montana State University for the USDA. The USDA has already invested at least $23 million in this research. The UNDCP has not dropped its endorsement of the plan. The spraying plan in Colombia does, however, appear to be at least dormant at the present.
A variety of different dispersal methods have been suggested. The most direct form would be aerial spraying of the spores. The lead US researcher that developed the EN-4 strain, David Sands, proposed to equip large transport aircraft to spray tons of F. oxysporum spores from specialized equipment attached to the bottom of the plane.
The idea to disperse the agent from a high altitude is intended to blanket large areas and to avoid ground-based gunfire. Forced eradication efforts – especially in war situations such as in Colombia or some Central Asian areas – are likely to meet armed resistance, and several chemical eradication pilots have been killed in the past. Sands favours spraying huge areas where coca is currently grown and, as a preventative measure, over areas where coca could be sown.

Fusarium is passed through seed! That means seeds are not safe anymore!

"If seed is taken from infected plants, the seed itself is usually healthy, but the seed coat often becomes contaminated by microscopic pieces of infected tissue and by spores. Many important Fusarium wilt diseases are spread in this manner. It is always prudent to treat seed with a fungicide or heat to destroy the fungus on the seed and to protect the emerging seedlings from infection."
Fusarium is definitely a fungus that has, at least, been implicated in government funded research for the eradication of drug crops. It has been suggested that this stuff has been sprayed in Colombia and possibly here in NA. Fusarium has a lot of variations. The one talked about below as the "pot eating fungus" is Fusarium Oxysporum and I don't know how different they are.

Tom Hill mentioned something about seeing the feds spraying botyritis, I believe. Wouldn't put anything past the DEA.

 

[Sam_Skunkman]

WOW,
this is the best news I have seen yet, I wonder if we can find more labs that will test Cannabis and then do a check of all the testing services by sending the exact same problems to all the labs that test?
If their results all match we are on the road to beginning to have a way to ID pests and pathogens, we just need the best labs to add all the pests and pathogens known to be found in Cannabis, starting with the biggest problems first.
Did you ask the lab about BM toxins? That I would like to understand.
Was Purple Kush both positive and negative?
-SamS

 

[mofeta]

Thanks RetroGrow, that is great news. It looks like answers will be forthcoming.

I was wondering when the first mention of some government or corporate sabotage would occur, I can't believe it has taken this long. Are the other forum's members speculating along these lines in regards to duds, or is this your own personal feeling? Nothing personal, but I hope people don't latch onto that idea, it is irrelevant to what I am interested in, and could cause the rapid decay of this now pretty good thread.

Anyway, thanks again for that news.

 

[RetroGrow]

WOW,
this is the best news I have seen yet, I wonder if we can find more labs that will test Cannabis and then do a check of all the testing services by sending the exact same problems to all the labs that test?
If their results all match we are on the road to beginning to have a way to ID pests and pathogens, we just need the best labs to add all the pests and pathogens known to be found in Cannabis, starting with the biggest problems first.
Did you ask the lab about BM toxins? That I would like to understand.
Was Purple Kush both positive and negative?
-SamS

Re: Purple Kush-The +- means "borderline" positive.
Don't know exactly what that means.
To make it work, more people need to get their samples tested, ie., people with "duds" need to send them in and specify the tests you want performed.

 

[RetroGrow]

Thanks RetroGrow, that is great news. It looks like answers will be forthcoming.

I was wondering when the first mention of some government or corporate sabotage would occur, I can't believe it has taken this long. Are the other forum's members speculating along these lines in regards to duds, or is this your own personal feeling? Nothing personal, but I hope people don't latch onto that idea, it is irrelevant to what I am interested in, and could cause the rapid decay of this now pretty good thread.

Anyway, thanks again for that news.

This isn't the first time the government has been mentioned. Many have mentioned it. The government is always my first suspect, because our government has a history of this type activity. It started with Nixon & paraquat. Now they have weaponized fusarium that can be sprayed over wide areas by planes. This is pretty scary stuff. I put nothing past the DEA. I've seen how they operate up close and personal. Not trying to turn this into a conspiracy thread, just sayin'.

 

[Elmer Bud]

G`day Retro

Ole Billy took the opportunity of the distraction of the Lewinsky affair to appologise for MK Ultra ...

http://www.youtube.com/watch?v=KRTOB8JPwa8

How about Tuskegee syphilis experiment

Merica ! Land of the free .

Thanks for sharin

EB

 

[xxxstr8edgexxx]

This isn't the first time the government has been mentioned. Many have mentioned it. The government is always my first suspect, because our government has a history of this type activity. It started with Nixon & paraquat. Now they have weaponized fusarium that can be sprayed over wide areas by planes. This is pretty scary stuff. I put nothing past the DEA. I've seen how they operate up close and personal. Not trying to turn this into a conspiracy thread, just sayin'.

the government involvment is a fascinating component of all this, you should start a thread about it. the dea dud conspiracy thread. maybe we could keep this thread a resource about diagnostics prevention and cure regardless of where it originated. its not tht its not a worthwhile topic but i think this thread could turn unmanageable and be hard to use as a resource in the future for people trying to identify and solve this syndrome.

 

[Storm Shadow]

http://www.entomology.umn.edu/cues/Web/153HoneysuckleWitchesBroomAphid.pdf

HONEYSUCKLE WITCHES' BROOM APHID

 

[Storm Shadow]

http://www.apsnet.org/edcenter/intropp/PathogenGroups/Pages/Fastidious.aspx

Fastidious Vascular-Colonizing Bacteria

The Mollicutes: Phytoplasmas and Spiroplasmas

 

[Elmer Bud]

Not Cannabis but a model of the mite - virus vector .

And a mite rampant in Sri Lanka where a lotta coco comes from .

coconut mite

What Causes Witches Broom in Roses?

The virus is believed to be spread by tiny mites that can carry the nasty disease from bush to bush, infecting many bushes and covering much territory. The mite is named Phyllocoptes fructiphilus and the type of mite is called an eriophyid mite (wooly mite). They are not like the spider mite most of us are familiar with, as they are far smaller.

Miticides used against the spider mite do not appear to be effective against this tiny wooly mite. The virus does not appear to be spread by way of dirty pruners either, but only by the tiny mites.

Research indicates that the virus was first discovered in wild roses growing in the mountains of Wyoming and California in 1930. Since then it has been a case for much study at plant disease diagnostic labs. The virus has recently been placed into a group known as Emaravirus, the genus created to accommodate a virus with four ssRNA, negative sense RNA components. I won’t go further into this here, but look up Emaravirus online for further and interesting study.

 

[RetroGrow]

the government involvment is a fascinating component of all this, you should start a thread about it.

You can start a thread about it if you find it so fascinating.
Wasn't my intention, even though it's not off topic.