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Radiation mutation breeding . . .

foomar

Luddite
ICMag Donor
Veteran
Get the distinct impression that GM cannabis might not go down too well . .

How do you feel about mutation breeding with radiation ?

Acceptable or a dead end ?

It has a long and successful history with a number of common food crops , and it only increases the incidence of a natural process that has driven evolution.

Downside is you still need to grow out thousands to find a useful non lethal sport , and unlike GM its more or less random.

Deleting or recombining the existing DNA should have less opposition than transgenics , and could produce some interesting variants.


Ionizing radiation includes ultra-violet (UV) light, X-ray, Gamma rays, and neutrons. These high-energy forms of radiation cause double-strand breaks of the DNA double helix. Once pieces of the DNA are broken, cellular repair mechanisms stitch the pieces back together. These DNA repair systems can only handle low rates of radiation, however, and increases in the rate of exposure to ionizing radiation causes permanent mutations to occur and accumulate in an organism’s genome. Radiation causes deletions of nucleotides from the DNA sequence. These deletions can cause reading-frame shifts, inactive protein products, or faulty transcripts. This typically results in null mutations, which are those in which a particular gene is inactivated.
 

non

Active member
Veteran
i don't know shit and that being said, this sounds interesting in theory. for the hippie-type it just sounds too evil, well expect maybe if you take the seeds/clones(seeds only?) to space where they can get a dose of organic primordial radiation.
 
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foomar

Luddite
ICMag Donor
Veteran
Might sound evil , but you have almost certainly eaten some.

Though poorly known, radiation breeding has produced thousands of useful mutants and a sizable fraction of the world’s crops, Dr. Lagoda said, including varieties of rice, wheat, barley, pears, peas, cotton, peppermint, sunflowers, peanuts, grapefruit, sesame, bananas, cassava and sorghum. The mutant wheat is used for bread and pasta and the mutant barley for beer and fine whiskey.

The mutations can improve yield, quality, taste, size and resistance to disease and can help plants adapt to diverse climates and conditions.
 

foomar

Luddite
ICMag Donor
Veteran
Should be possible to find every previously documented genetic variation and some new ones , but the numbers needed to find them could be daunting from seed.

Remember seeing work done on hemp in the 30,s but cant find it right now.

Blackberry looks a good contender to have been a sport , possibly some others like the ducksfoot leaf trait ?
 

420247

Plant Whisperer
Veteran
Blackberry looks a good contender to have been a sport , possibly some others like the ducksfoot leaf trait ?

To go with your question...

"somatic mutation: It may be maintained by vegetative propagation but not by sexual reproduction"- People can make a new cultivar from a mutated sport...

What do you think about working with a mutant sport from a plant for breeding?

Say you take a Red Fuji mutant sport cutting/clone and use it for breeding... Would that create different offspring than using the unmutated Fuji apple cutting? These would be cuttings from the same tree... But 1 is mutated and the other is not... What are your thoughts?
 

HidingInTheHaze

Active member
Veteran
You should radiate yourself and see how it works.

If it doesn't work out too good when you radiate yourself it's probably not such a good idea to radiate your plants.

Human's and plants have a lot more in common then you might think, what's good for the goose is good for the gander.
 

420247

Plant Whisperer
Veteran
You should radiate yourself and see how it works.

If it doesn't work out too good when you radiate yourself it's probably not such a good idea to radiate your plants.

Human's and plants have a lot more in common then you might think, what's good for the goose is good for the gander.

What is a CT scan?

The CT scanner uses digital geometry processing to generate a 3-dimensional (3-D) image of the inside of an object. The 3-D image is made after many 2-dimensional (2-D) X-ray images are taken around a single axis of rotation - in other words, many pictures of the same area are taken from many angles and then placed together to produce a 3-D image.

The Greek word tomos means "slice", and the Greek word graphein means "write".

Although CT is a useful tool for assisting diagnosis in medicine, it is a source of ionizing radiation and can cause cancer. The National Cancer Institute1 advises patients to discuss the risks and benefits of computerized tomography with their doctors.

:laughing:
 

foomar

Luddite
ICMag Donor
Veteran
Have played with Blackberry crosses and have not managed to improve the incidence with normal crossing , don't think Nebus has managed to stabilise it and it seems to have disappeared.

Uncertain if Fuji could be classed as a mutation or a minor expression , either way it should pass on the traits to some variable extent.

Have Fuji on two different rootstocks , you would not think they were the same to look at them.

If an interesting cannabis mutation surfaced I would probably repeatably self it and work the line that way.


probably not such a good idea to radiate your plants

As radiation induced mutation has been the main driver for the variation that drives evolution I don't see it as a problem , its been a breeding tool for a long time.
Has to be better than GM , as its the same as happens in nature but speeded up.

There is a vast difference between the damage radiation does to an animal or plant , and the deliberate irradiation of a seed or pollen.
 

420247

Plant Whisperer
Veteran
Have played with Blackberry crosses and have not managed to improve the incidence with normal crossing , don't think Nebus has managed to stabilise it and it seems to have disappeared.

Uncertain if Fuji could be classed as a mutation or a minor expression , either way it should pass on the traits to some variable extent.

Have Fuji on two different rootstocks , you would not think they were the same to look at them.

If an interesting cannabis mutation surfaced I would probably repeatably self it and work the line that way.

The Fuji apple is an apple clone developed by growers at the Tohoku Research Station (農林省園芸試験場東北支場) in Fujisaki, Aomori, Japan, in the late 1930s,[1] and brought to market in 1962. It originated as a cross between two American apple varieties, the Red Delicious and old Virginia Ralls Genet (sometimes cited as "Rawls Jennet") apples.

Mutant cultivars
Many sports (mutant cultivars) of the Fuji apple have been recognized and propagated. In addition to those that have remained unpatented, twenty had received US plant patents by August, 2008:

Unpatented Fuji mutants include:

BC 2
Desert Rose Fuji
Nagafu 2
Nagafu 6
Nagafu 12
Redsport Type 1
Redsport Type 2

:ying:
 

Chimera

Genetic Resource Management
Veteran
It has been / is being done with Cannabis. However, radiation is less effective then EMS (Ethyl methanesulfonate) treatment; combined with TILLING it is a very useful strategy for inducing knockouts.


Google: "Targeted mutation of D12 and D15 desaturase genes in hemp produce major alterations in seed fatty acid composition including a high oleic hemp oil"

Plant Biotechnology Journal (2014), pp. 1–11

http://en.wikipedia.org/wiki/TILLING_(molecular_biology)

Hope that helps,
-Chimera
 
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foomar

Luddite
ICMag Donor
Veteran
Was unsure if it had any use in cannabis , but have ornamentals in oddball colours that were made through radio induced mutation.

The Japanese seem to be one of the main proponents of the techniques , some labs will treat seed at a price.

With care a Co 60 source would be useable at a diy level , but no chance of getting this nowadays without a lab and onerous paperwork.
 

foomar

Luddite
ICMag Donor
Veteran
That's not too expensive , a Japanese facility quoted five times that four years ago .
Growing enough out to find a useful one would still be the bottleneck.

Internal and external charges

Hourly rate for actual operator time: $20.00 / $40.00 1/2 -hour minimum charge
After Hours Hourly Rate: $40.00 / $80.00
Use Fee: $125.00 / $250.00
 

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