What's new

Creating an AF strain - 1st attempt

Really healthy lookin plant you got there. Like blackone said "great sogging structure" nice and stout. Good luck on the auto flowering aspect of it. If nothin else it looks like you'll end up with some killer bud.
 

blynx

WALSTIB
Moderator
ICMag Donor
Veteran
Seeds, seeds, seeds!


Day 40

picture.php


picture.php


picture.php


picture.php


picture.php


picture.php


About another month until she comes down
 
I

IceColdCrickets

good job blinx, cant wait to see the offspring. Well on paper, you will get 50% of them true Autoflowering. So the next step would be to grow out as many as possible and select the pheno that you will be trying to lock down in AF form. Try to match a male and female pheno that AF, starting strict selective breeding now will help the later generations. Too bad the Purple triat seems to have been pushed out with this plant. It may surface again, but doubt it. Due to the fact that purple is reccessive aswell and you just crossed it to 2 green flower dominant parents to get the PBS x SD x ML. Did you say you only have 3 seeds of the PBS x SD x ML and only grew out this one? The other 2 could contain the purple pheno you are looking for. It would be a good idea to go back and do the PBS x SD x ML to make sure, OR make some PBS x SD x ML F2s, inbreed the last 2 seeds if you are able to.

There are 2 ways to go about getting a 100% AF strain:
1. Most common in practice by some AF breeders is to make the initial F1 cross to a NON-AF plant x AF plant and then Backcrossing to the AF parent at F2s to make NON-AF x AF x AF, which is what you have done. The only draw back to this is you keep very little of the Original NON-AF plant that you enjoy so much. Now you have a genotype of 12.5% PBS x 12.5% SD x 75% MasterLow. Of course that is just on paper as genetics are never 100% exact.

2. The best way to make a New AF strain that is much different then the AF parent is to make an IBL program first off. This requires more plants to be grown out to find the AF parents later on, but it makes for a better hybrid with more of the NON-AF parents traits. Instead of Backcrossing to the AF parent make true F2s by inbreeding the initial F1 cross. So, PBS x SD x ML (female) X PBS x SD x ML (male), F2s, this keeps the genotype the same as the original F1 cross at 25% PBS x 25% SD x 50% ML. The resulting F2s will show more variation and a better chance to find the pheno you are looking for. But, as mentioned before it requires more plants to be grown out as your AF % in the F2s will be only 25%, half as much as backcrossing to the AF parent.

As you can see both methods can lead you to 100% AF plants by the F3 generation. Backcrossing is easier to find the AF trait and lock it down, but it is at the cost of losing genetic traits from the Orginal NON-AF parent.

I just wanted to throw this info out there, you may know it already, just giving a heads up to people that dont know.

Peace ICC
 

blynx

WALSTIB
Moderator
ICMag Donor
Veteran
good job blinx, cant wait to see the offspring. Well on paper, you will get 50% of them true Autoflowering. So the next step would be to grow out as many as possible and select the pheno that you will be trying to lock down in AF form. Try to match a male and female pheno that AF, starting strict selective breeding now will help the later generations. Too bad the Purple triat seems to have been pushed out with this plant. It may surface again, but doubt it. Due to the fact that purple is reccessive aswell and you just crossed it to 2 green flower dominant parents to get the PBS x SD x ML. Did you say you only have 3 seeds of the PBS x SD x ML and only grew out this one? The other 2 could contain the purple pheno you are looking for. It would be a good idea to go back and do the PBS x SD x ML to make sure, OR make some PBS x SD x ML F2s, inbreed the last 2 seeds if you are able to.

Thanks.

The purple trait in the plant I used (PBS x SD) doesn't come out until the end of flowering and then it's usually just the calyxes. Cooler temps will greatly brighten the purple in the plant and start to turn smaller fans purple too.

I'm sure the purple is recessive. The mom (PBS) had the purple calyxes like the PBS x SD (it was plant 4 so I call it PBSD4) that I used in this cross.

I had another PBS x SD (plant 3, ie PBSD3) plant going for a while, but it didn't have the purple trait in it.

I don't have any more PBS x SD pollen at the moment. I would have to grow out more PBS x SD seeds, find a male and collect it. So currently all I have is 2 (PBS x SD) x ML seeds left.

I pollinated my PBSD4 with ML F2 pollen over a month ago and just harvested the seeds the other day, so now I do have over a hundred (PBS x SD) x ML F2 seeds. These ML F2s were not made from the ML pollen used to make the 2 (PBS x SD) x ML seeds mentioned above.

I also just harvest some Lady G x ML F2 and The White x ML F2 seeds that I made the same day as the (PBS x SD) x ML F2 seeds.


There are 2 ways to go about getting a 100% AF strain:

Yep I had planned on trying both.

1. Most common in practice by some AF breeders is to make the initial F1 cross to a NON-AF plant x AF plant and then Backcrossing to the AF parent at F2s to make NON-AF x AF x AF, which is what you have done. The only draw back to this is you keep very little of the Original NON-AF plant that you enjoy so much. Now you have a genotype of 12.5% PBS x 12.5% SD x 75% MasterLow. Of course that is just on paper as genetics are never 100% exact.

I figured this way would be the easiest and quickest way for a first attempt at creating 100% AF seeds.

2. The best way to make a New AF strain that is much different then the AF parent is to make an IBL program first off. This requires more plants to be grown out to find the AF parents later on, but it makes for a better hybrid with more of the NON-AF parents traits. Instead of Backcrossing to the AF parent make true F2s by inbreeding the initial F1 cross. So, PBS x SD x ML (female) X PBS x SD x ML (male), F2s, this keeps the genotype the same as the original F1 cross at 25% PBS x 25% SD x 50% ML. The resulting F2s will show more variation and a better chance to find the pheno you are looking for. But, as mentioned before it requires more plants to be grown out as your AF % in the F2s will be only 25%, half as much as backcrossing to the AF parent.

I like this method better too, it just takes a bit more time and room than the other method.

As you can see both methods can lead you to 100% AF plants by the F3 generation. Backcrossing is easier to find the AF trait and lock it down, but it is at the cost of losing gentic traits from the Orginal Non-AF parent. I just wanted to through that info out there, you may know it already, just giving a head up to people that dont know.

Peace ICC

Thanks for the nice summation of the 2 methods, I appreciate the post.
 

jay-toker

Active member
wow bro looks like your on the right track and iv been wanting to do what ur doing and was going to but my DR's have not grown in a month for some reason and now have no other af seeds. i would like to get my hands on some ml seeds ill have to find a site that ships to the us witch is hard cuz iv looked around alot if u know a site or someone let me know please.

peace toke out
 

blynx

WALSTIB
Moderator
ICMag Donor
Veteran
It is day 46 but I figure it still has a couple weeks left.

Day 46

picture.php


picture.php


picture.php


You can see the seeds are still green.
 

9Lives

three for playing, three for straying, and three f
Veteran
Mmm great thread...im sticking around for sure. Good Luck!
 
H

h^2 O

:yes:
Good work blnyx
I liked the diesel on the 1st page, frost nugz galore
 

blynx

WALSTIB
Moderator
ICMag Donor
Veteran
She's losing her fans, but still needs some more time.

Day 54

picture.php


picture.php


picture.php


picture.php


picture.php


picture.php


picture.php


picture.php


picture.php



Back side of the cola

picture.php


picture.php


picture.php


Getting closer....:woohoo:
 
C

cyber echo

Thats a nice plant. I'd like to see what LST does with this "one-bud" plant. Tho thats too late for now for sure. Im going to be growing an AF LST'd soon (when the seeds arrive). I'll document it.
 

blynx

WALSTIB
Moderator
ICMag Donor
Veteran
No amber trichs yet, but then again, I'm not really going by trichs with this plant, I'm going by when the seeds are done. And it looks like they still have some time left.

Day 59

picture.php


picture.php


picture.php


picture.php


picture.php



I was hoping to take her down around day 63, but I will probably have to let her go to around day 70 to make sure that most if not all the seeds are done ripening.
 
C

cyber echo

Thanks for the info cricket.
You discussed Non-Af x AF. How about AFxAF ?
Perhaps this isnt the correct threat to discuss this tho.
Feel free to open up a thread and expound some more genetic info :p
I think it'd be a sticky for sure.

Sorry for hijacking ur thread blynx.
Cheers
 

Latest posts

Latest posts

Top