Chocolate Thai Revival

[Roms]

Yeah wet 25°C is optimum before and after seed is open, spring mineral water long float before too but i think that it's better for old seed to be direct in the soil after opening than waiting too long in moist PT. Also use good wattage from the start and not little set up nursery.

Good luck again Zanddar!

Gardening Moon Calendar 2022, Biodynamic Farmer's Guide

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mooncalendar.astro-seek.com

 

[acespicoli]

Drawoh- done by hazeman CT F4

 

[acespicoli]

Cannabis Seed Storage

quick wash in hydrogen peroxide is a bonus for any seed planted? Soak your seeds in 3% hydrogen peroxide for 30 minutes. Rinse the seeds several 3X times with DISTILLED water before planting and plant them as usual. Doing this breaks down the hard outer covering of the seeds and kills any...

www.icmag.com

Check this out or other sources on embryo rescue might help stubborn seeds
if you can get a tail and have signs of at least a single cell of veg life, go invitro
worst fear is once its gone its gone forever, worth the effort

Embryo rescue - Wikipedia

en.wikipedia.org

Plant tissue culture - Wikipedia

en.wikipedia.org

 

[Thcvhunter]

When I popped seeds from the 70s a few yrs back i did this.

Soak in peroxide for 5 minutes. Rinse.

Soak in 50/50 water / Fulpower. Add a couole drops EM1.
Let em soak overnight, or several hours after they sink.
Remove then place on papertowels to slightly dry out. The idea being theyre gonna need to be wet for a while, so lets not let them rot. I pour them into paper towels and when the towels start to dry (just less than moist but far from dry) i put them on soil. The soil i wet before the seeds go in, then sprinkle down a bit of earthworm castings. Seeds sit on top. Mist with a sprayer til they sprout

 

[Zanddar]

I looked at some other seeds in the fridge and the CT were there looking back at me. I plan on trying them again soon.

Thanks for all the tips and will keep trying. Let you know soon what I will do.

 

[Zanddar]

Drawoh CT F4

Have some of these seeds to do too.

 

[*GROWHIGH*]

its really a shame that none of the CT have been viable so far ....... i would love to see what's in those genetics ..........the most knowledgeable person i can think of on this subject is @Chimera ...tho i dont think he posts much here anymore ...i think he posts on twitter and insta tho ..someone should drop him a message ....hes a real scientific son of a gun

 

[Zanddar]

Whatever it takes and I still have a handful.
The shoots came out before, so we know some are still good.
Buddy said it looked like crap, but was the best weed around at the time.
The guy that brought it in is dead now. All that remains are these seeds and memories.

 

[Zanddar]

T for Thai and T for Try. 40 years old and trying.

What I did this time is firmly roll the seed between my fingers to see if it turned to dust first and many of them did. I'm not going to get anywhere with dead and dried out seeds. My fingers got sore rolling the good seeds though. Then I gave them a 5 min alcohol soak to clean the outside. Then I scuffed the edges to make it easier for the shoot to come out. Then I will give them a 24hr soak in organic food solution. Then they go directly into soil and we wait a month if it takes that long. I have 10, but it only shows 9 in pic.

 

[acespicoli]

 

[Zanddar]

They all sank and are in soil.

 

[*GROWHIGH*]

They all sank and are in soil.

sounds like you've been very thorough,...i guess its in the hands of the seed gods now ........wishing you luck brother

 

[Zanddar]

Nothing from the other 10 that are still in soil and starting another 11 soaking.
Some of these even look green. So lets try Thai again.

 

[acespicoli]

the tease is on... choc thai very few people work that one
I think you should check into the TZ staining to see if your working with viable material

 

[Zanddar]

T is for tease.

That test is interesting, but some have germed before.
They just failed to continue to grow further than the shell.

 

[acespicoli]

The one percent h202 wetting will make sure the old seed is not over come by fungi or bacteria

https://www.researchgate.net/figure/Germination-of-cannabis-seeds-in-1-hydrogen-peroxide-solution-and-water-a-Comparison_fig1_342259283

#176

Then you want to soak with M&S nutes $5 USD makes litre

To sterilized fine vermiculite
shot cup size after that there should be enough vigor to tap compost
if there is one living cell it is possible to replicate to full size plant given time and growth hormones
GWE Tissue Culture

 

[Zanddar]

I was using iso, but switched to h202 to give them more oxygen.

 

[@hempy]

T is for tease.

That test is interesting, but some have germed before.
They just failed to continue to grow further than the shell.

The hormones and enzymes stored in the seed between the embryo and embryo case dry up in old seed and why they dont germinate or go past throwing a small root. Some seed will even split and look like they are about to germ but re close and die.

Cleaning the old seed in a wash i think 3% peroxide ( you need to check that ) is important to remove and contaminants.

Then you need to replace the lost hormones and enzymes.

The coconut water mix works well to increase old seed germination but even then when the seed are to old it to will fail.

Best chance to get them up is using something like

GA3 Gibberellic Acid.​

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[Zanddar]

Ok time to try the GA again a bit stronger.
First I gave them a rinse in H2O2 and then they soak in GA3.

 

[Redrum92]

Have you tried soaking them in the fridge? Was something I learned germinating other species of seeds. I imagine it simulates a spring thaw for a seed, and gives the embryo a headstart on germinating ahead of bacteria. ~36 hours in the fridge, in water (either a cup, wet paper towel, whatever). I do all my seeds this way, and it seems to help. I also put a drop of clonex juice in if they're older seeds- B vitamins and hormones like others have recommended

If you've already tried this, apologies. Been quite a long thread. Wishing you luck