The apical meristem of a plant is always clean,,,the idea here is to prevent it from becoming infected with treatment and prior surface cleaning, we then isolate with an explant,,,dumping the original plant stock,,
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Who is our resident Tissue culture master?
- Thread starter grod31
- Start date
Mudballs2.0
Active member
it's weird..double strand DNA can withstand it, single strand RNA cannot. What im seeing inline with englishricks idea, is just short treatments...like 1 hr. Idk i. Just now trying to get up to speed with him since last night lol
Would Physan 20 work to sterilize plant material?
Do you know how many inches is always clean, and is that just the main stem top growing point or side branching also.
NEED 4 SEED
Well-known member
Im pretty sure it's on every shoot where new growth happens but I would like to know as well how much of the new growth is considered free of pests tissue.
Mudballs2.0
Active member
what he's discussing is apical meristem, this is a root pic but it works the same to represent the growing branch tips.
Do you know how many inches is always clean, and is that just the main stem top growing point or side branching also.
not far at all, they literally shave that tip like shaving an onion for the skillet.
Do you know how many inches is always clean, and is that just the main stem top growing point or side branching also.
not far at all, they literally shave that tip like shaving an onion for the skillet.
I'd be interested to know if T.C. could actually be performed where you used root tips rather than the top 1-3 mm of plant growth site.
Root tips would offer more protection from infection than anywhere else.
www.ncbi.nlm.nih.gov
Root tips would offer more protection from infection than anywhere else.
Extracellular DNA Is Required for Root Tip Resistance to Fungal Infection
Plant defense involves a complex array of biochemical interactions, many of which occur in the extracellular environment. The apical 1- to 2-mm root tip housing apical and root cap meristems is resistant to infection by most pathogens, so growth and gravity ...
www.ncbi.nlm.nih.gov
NEED 4 SEED
Well-known member
From what I've read roots or tips should work the sameI'd be interested to know if T.C. could actually be performed where you used root tips rather than the top 1-3 mm of plant growth site.
Root tips would offer more protection from infection than anywhere else.
Extracellular DNA Is Required for Root Tip Resistance to Fungal Infection
Plant defense involves a complex array of biochemical interactions, many of which occur in the extracellular environment. The apical 1- to 2-mm root tip housing apical and root cap meristems is resistant to infection by most pathogens, so growth and gravity ...
Rico Swazi
Active member
Still processing this paper on bacterial/fungal endophytes
I see a definite need and huge market for inexpensive PCR testing kits for the home gardener.
www.frontiersin.org
I see a definite need and huge market for inexpensive PCR testing kits for the home gardener.
Frontiers | Variables Affecting Shoot Growth and Plantlet Recovery in Tissue Cultures of Drug-Type Cannabis sativa L.
Tissue culture approaches are widely used in crop plants for the purposes of micropropagation, regeneration of plants through organogenesis, obtaining pathog...
www.frontiersin.org
DIY Tissue Culture - Why You Should Be Trying It
How to do DIY tissue culture cannabis growing. Read more.
www.plantcelltechnology.com
NEED 4 SEED
Well-known member
As they said, the critical point are protocols for cannabis tissue culture. Where can we find them?DIY Tissue Culture - Why You Should Be Trying It
How to do DIY tissue culture cannabis growing. Read more.
When I get chance il put the kit out,,,I promise,,,il explain everything and give you all the bits needed
Roots also have totipotency,,so yes roots can grow into a full plant,,,roots need to go through indirect organogenesis,,,meaning it will turn to callus (undifferentiated cells) and then through differentiation it will grow shoots or roots depending on the mediums ratios of auxin to cytokinins
Roots also have totipotency,,so yes roots can grow into a full plant,,,roots need to go through indirect organogenesis,,,meaning it will turn to callus (undifferentiated cells) and then through differentiation it will grow shoots or roots depending on the mediums ratios of auxin to cytokinins
You are absolutely correct,,thanks for the images,,I probably wouldn't bother myself so It helps alot ,,
When we talk about meristem we talk about normal cloning,,big ones,,,even microcloning is basically just normal meristem cloning, same as big clones,,,but when we talk about the apical meristem,,magic worlds being APICAL MERISTEM,, we talk about a tip so small you need a 50x+ stereoscopic microscope to do catch it,, notice one in the picture of my kit,,,
The apical meristem is Always clean,,the idea with the deeper end of tissue culture is to isolate and culture it,,
Attachments
Mudballs2.0
Active member
Some of us got to talking about that heat treatment concept and i think we have one fella that's possibly gonna try it. He decided to dip infected clones in 158degree hot water over a series of days and then if it lives, send in for testing.
I want to thank you for being a sharing individual.
Have you seen any data on hops latents sensitively to microwave?
Mudballs2.0
Active member
Not for this particular RNA
I guess we could extrapolate and correlate...bro, i hate math..much less calculus
I guess we could extrapolate and correlate...bro, i hate math..much less calculus
Great conversation guys, great links and a wealth of information.
My first TC jars are in the PC right meow. The directions say 20 mins but doesn't say at what PSI so I just went to 15. I hope 40 mins is okay cause I have other stuff in there that needs that long. I only used half the media incase I fucked it all up.
My first TC jars are in the PC right meow. The directions say 20 mins but doesn't say at what PSI so I just went to 15. I hope 40 mins is okay cause I have other stuff in there that needs that long. I only used half the media incase I fucked it all up.
Rico Swazi
Active member
Will that kit include RT-PCR?
'The apical meristem is Always clean' statement of yours has me confused
is this before or after initiation?
How could you know degree of cleanliness without a test is what I'm asking
I wasn't planning on putting pcr tests in the kit ,,I could do with understanding testing a lil more,
In tissue culture ,we atempt to isolate the apical meristems because they are always clean,,,apical meristem tissue has not had chance to become infected,,older tissue becomes infected quickly and to avoid infected tissue we isolate the apical meristem,,,does this make sense to you now?
Initiation is the process of bringing genetics into a tissue culture environment like invitro (in glass) testubes etc,,,,,initiation can start in the growroom prior to surface sterilisation, its this prior initiation work i was referring to in my previous posts,,
we take precautions like initiation, because of potential slips,,,we take precautions at all levels to prevent the possibility that when cutting tissue we might not avoid primordia or might not shave something enough,,small slips will fuk everything up, but if you take precautions at all levels then slight mistakes won't come back to haunt you,,,sometimes you can take the work too far,,,my previous post try to mediate what is entirely necessary
In tissue culture ,we atempt to isolate the apical meristems because they are always clean,,,apical meristem tissue has not had chance to become infected,,older tissue becomes infected quickly and to avoid infected tissue we isolate the apical meristem,,,does this make sense to you now?
Initiation is the process of bringing genetics into a tissue culture environment like invitro (in glass) testubes etc,,,,,initiation can start in the growroom prior to surface sterilisation, its this prior initiation work i was referring to in my previous posts,,
we take precautions like initiation, because of potential slips,,,we take precautions at all levels to prevent the possibility that when cutting tissue we might not avoid primordia or might not shave something enough,,small slips will fuk everything up, but if you take precautions at all levels then slight mistakes won't come back to haunt you,,,sometimes you can take the work too far,,,my previous post try to mediate what is entirely necessary
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Unfortunately I suspect you'd need to know what you were testing for. Each undesirable would have its own markers making a universal test difficult if not impossible.
Prior samples could be sent for analysis and then PCR kits ordered for that issue I guess, if you live somewhere legal. Costly though.
Prior samples could be sent for analysis and then PCR kits ordered for that issue I guess, if you live somewhere legal. Costly though.
