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As the first poster said once is enough if done right but adding more over time won't hurt it will just cost you a bit more for each supply of Mycofungi.
On my SWICK, only pH and cal-mag (or epsom salts) go in my reservoir. Water stays clean that way. I dip a gallon jug in the reservoir for water, mix up my microbes and whatever feed the plant gets, wait the 30 minutes for the microbes to multiply, and top water on the 10 gallon bag. About 10% water to soil ratio.