http://www.pakbs.org/pjbot/PDFs/41(2)/PJB41(2)603.pdf
Abstract
This study describes the standardization of an efficient in vitro propagation and hardening
procedure for obtaining plantlets from shoot tips of Hemp (Cannabis sativa L.). Hemp seedlings
were germinated on half-strength 1/2 MS medium supplemented with 10 g·L-1sucrose, 5.5 g·L-1agar
at a pH of 6.8 under light for 16 h per day. MS medium containing 0.2 mg·L-1TDZ, 0.1 mg·L-1NAA
supported the maximal auxiliary bud multiplication rate of 3.22 per shoot tip. The proliferated buds
were successfully rooted on MS medium supplemented with 0.1 mg·L-1IBA and 0.05 mg·L-1NAA
resulting in 85% of the plantlets rooting. The procedure requires a 54 days cycle for the In vitro
procedure for obtaining plantlets from shoot tips of Hemp (Cannabis sativa L.). Hemp seedlings
were germinated on half-strength 1/2 MS medium supplemented with 10 g·L-1sucrose, 5.5 g·L-1agar
at a pH of 6.8 under light for 16 h per day. MS medium containing 0.2 mg·L-1TDZ, 0.1 mg·L-1NAA
supported the maximal auxiliary bud multiplication rate of 3.22 per shoot tip. The proliferated buds
were successfully rooted on MS medium supplemented with 0.1 mg·L-1IBA and 0.05 mg·L-1NAA
resulting in 85% of the plantlets rooting. The procedure requires a 54 days cycle for the In vitro
clonal propagation (14 days for shoot multiplication and 40 days for root induction) which includes
35-42 days for acclimatized plantlet production.