#1 is reversing a male possible with similar procedure of reversing a female?
ethephon used on the plant material is converted very quickly to ethylene. I smoked transformed male to female, I do not think it is dangerous at all. I used pure ethephon.
-SamS
DP was the first to sell all fem seeds made with STS, Hybe was one of the first selling STS for $100 a liter, but we were the first to use STS, we used it shortly after reading about it in Ram's paper, I and Rob Clarke read Rams paper in 1983 and made and used it a few years later.
At the time I was hoping to combine all female seeds with all sterile plants that could not set any seeds.
We were also amongst the first to turn males to females with ethephon (2-chloroethanephosphonic acid). So the male that was female transformed could be tested for Cannabinoid and terpene contents, it worked great.
With all female sterile seeds, then anyone could grow sinsemilla, no need to sex plants, and even if your neighbor had a zillion flowering males you would get no seeds in your sterile all female crop. Perfect for Morocco hash farmers that never see sinsemilla grown in the Rif, just to many male plants.
Anyway, all female is easy, making your all female seeds also sterile I never succeeded with although I tried for 3 years using a method that watermelon seed producers use to make seedless watermelon's. It almost worked but still made white nubbies and a few to a lot of black seeds no matter how I tried or what genetics I used.
-SamS
#2 Will reversing a male provide progeny that are 100% male?
I have read that the males transformed to female have a high incidence of sterility and have trouble making seeds, but that is unimportant if you just want to judge your male to determine it's suitability for breedin with your elite female clones.
-SamS
I have heard that this method make females that are often sterile, but even if not sterile, to make all male seeds is not so simple as this. With females that are turned male there is no Y only XX so you get all female seeds, when a male XX is crossed with a normal XX female. With a male turned female both X&Y are there so I doubt a female transformed to male will make all male seeds. Maybe you need to find or make a super male YY and cross it times a normal XX female, you should get all XY, male? I think so.
-SamS
XY = male
XX = female
Females turned to male have only XX so when bred to a normal female XX the result is always XX, female.
Males turned to female are XY so when bred to a normal male XY the results are half the seeds are XY male, and 25% are XX female, And 25% YY male if they can survive, because YY often do not.
-SamS
Try this , use XY x XY as the parents.
https://www.changbioscience.com/genetics/punnett.html
Punnett Square
XY x XY
...X Y
X XX XY
Y XY YY
Genotype Frequencies:
XX: 1 ( 25% ) female
XY: 2 ( 50% ) male
YY: 1 ( 25% ) male if it lives
Justin Ma, PhD in Plant Breeding and Genetics
I read the whole thread last year, :
Reversed male + male = 100% male seeds, right?!
I want to hunt a good male, but in this case I think plants will be very uniform... or nice differences can be found?
@Ibechillin, we have posted at the same time
ok for flowers traits better than seeds! I just remember now seeds is not an easy to make.
I think the question was not about femmed seeds; but about "feminized males"."Femmed" seeds are 99.9% female, because you're forming male flowers on a female plant.
The question is about producing malinized(made up word. lol) seeds for 99% male plants. This is not possible by simply reversing a male and pollinating it with male pollen. Hence the explanation of how feminized seeds work the way they do.I think the question was not about femmed seeds; but about "feminized males".
The question is about producing malinized(made up word. lol) seeds for 99% male plants. This is not possible by simply reversing a male and pollinating it with male pollen. Hence the explanation of how feminized seeds work the way they do.
Not an organic chemist, but here’s the basics as I understand it:
ALL females are X/X - two chromosomes, both Xs
ALL males are X/Y - two chromosomes, one X and one Y
Inducing pollen production in females only has X chromosomes to work with: all the pollen will be X, all seeds resulting will be X/X
Inducing flower production in males has both X and Y chromosomes to work with, but I’m pretty sure that flower itself cannot create an X calyx; if induced flowers *can* provide a Y chromosome, I dunno, but suspect it would be sterile or un-germ-able. So-called “super” males in humans are X/Y/Y...can’t even imagine what an YY plant would be like, since all sexual reproduction has X as the base.