Dud Identification Collective Knowledge.

[Storm Shadow]

Like I said b4... I have photos on here of a 24k lighter with Tahoe OG Kush from Progressive Options...the Kingdom of all these Bugs/Diseases/Viruses... Not one plant duded out and everything was super dank... again next round...same room... green crack... different treatments... 95% DUDS...

I noticed Fungus Gnats... and what I thought was a Katydid most likely a Leaf Hopper

lets hear from other people who have this problem...and not from naysayers who have zero experience with this issue

 

[pappy masonjar]

G`day Storm Shadow

Although you have posted lots of informative articles .
Do you think that Witches Broom is what is effecting the plants ? I see no evidence of Witches Broom in the pix of duds people have posted . Low vigour yes . Less than fully formed trichs yes . But Witches Broom . Symptoms . No .

What about the picture of the plant with zero nodes on that one branch. Just a profusion of leaves? (I think HL45 posted it)
I realize true "witches broom" may just be stems(?), but that picture seemed witch broomy to me

So far you have linked us to lots of info re plant pathogens . Not one that actually is related to Cannabis ...

Didnt stormshadow post 2 articles about asters yellow in cannabis in china and iran?
And mofeta also posted them

Sam keeps tellin you guys get the plants to a lab , find out what it actually is . Then no need to read 50 PDF`s about things that may not even be the cause . And or treatments for bugs / pathogens that you can`t identify .

Some of the links were quite informative . Prolly quite useful if thats the specific crop you are growing . Other wise not relevant .

Thanks for sharin

EB .

Mines in bold

 

[RetroGrow]

Progressive Options...the Kingdom of all these Bugs/Diseases/Viruses.

Which begs the question: why do you continue to go in there, supporting them and helping to vector their diseases?

 

[Elmer Bud]

G`day Pappy

Didnt stormshadow post 2 articles about asters yellow in cannabis in china and iran?
And mofeta also posted them

Cannabaceae = Cannabis / Hops , that pic is of a ? [Celtis (hackberries) ]

If Storm Shadow did post re Cannabis I apologise . It is a case of Too long didn`t read . After reading all the other wild goose chases .

@ Storm Shadow
I appreciate the energy you are expending looking for a cure . Hours of reading and digesting facts .
I think your time would be more productive . Being used to take the time to take a sample to a lab . Then attack the problem with acute knowledge. Not the AC130 U strategy you are now employing .

Thanks for sharin

EB .

 

[Elmer Bud]

STUDS and DUDS

Those that know the symptoms will notice this look right away

ASTER YELLOW DISEASE

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G`day Pappy

I see these pix .
No science to back it up . So who knows WTF is actually the problem ?

Then when some one asked a question re the pix . No reply .No explanation . But 10 more links .

re High Lighters pix . They show diff symptoms to Storm Shadow`s .He had no flower sites . SS has chlorosis of his flowers . So what is the relationship ?

The same disease at different stages ??

Thanks for sharin

EB .

 

[siftedunity]

there's definitely nothing wrong with exploring texts and looking at the problem by different angles. on the other hand even if symptoms have a text book cause, ultimately its only gonna be proven by testing in a lab.
ive enjoyed reading much of the links in this thread, but someone needs to send a dud off for testing.

 

[siftedunity]

Some of the links were quite informative . Prolly quite useful if thats the specific crop you are growing . Other wise not relevant .

Thanks for sharin

EB .

many plant issues spread between different species of plant so although not directly base don cannabis, it definitely has relevance. theres' not a great deal of scientific literature on cannabis compared to food crops, so for the time being its the best comparison we have.

 

[Elmer Bud]

G`day Sifted

Googling a term eg Witches broom disease or Phytoplasma and then cut and pasting every link doesn`t refine the search for the cure .

Good reads no doubt. I learnt a lot about pythium and fusarium . But also learnt a lot of stuff that is irrelevant to cannabis growing . My brain only has so much storage space . It needs to be used efficiently ...

Thanks for sharin

EB .

 

[Storm Shadow]

http://www.uaex.edu/farm-ranch/pest...sletters/plant health clinic newsletter 1.pdf

Phytoplasma are specialized bacteria that invade plant phloem tissue and cause disease. Sap-sucking insects transmit phytoplasma from plant to plant. Four families of planthoppers and two genera of psyllids, as well as leafhoppers are known to be vectors of phytoplasmas. The bacteria enter the insect’s body through the stylet when feeding on an infected plant. From there, they move through the intestine, and eventually colonize the salivary glands. When the insect feeds on an uninfected plant, the bacterium is transmitted to the plant. Phytoplasmas may also be transmitted by parasitic plants such as field dodder. Symptoms include leaf yellowing, smaller than normal leaves, stunting, witches’ broom, dieback, poor root growth, and sometimes plant death. A very common symptom is phyllody, the production of leaf-like structures in place of flowers. Many types of plants are susceptible to phytoplasma diseases, including, ornamentals, and weeds, fruit and vegetables crop

 

[Elmer Bud]

G`day Storm Shadow

Google search term for you - Furalaxyl .

Thanks for sharin

EB .

 

[Yesca73]

very interesting thread . I might have had some duds or something last year. I was growing Green Crack and Kryptonite. about 24 plants on ebb n flow while most plants looked good there was 2 of each strain that were smaller , more spindly , weaker stemmed , and buds didn't turn out like they normal due and plants stayed smaller than others, they didnt look happy. I did have some fungus gnats and maybe trips. I looked at the roots after harvest and couldn't see any bugs. I thought maybe they were bad clones that never got up to speed as the others.

 

[Sam_Skunkman]

Like I said b4... I have photos on here of a 24k lighter with Tahoe OG Kush from Progressive Options...the Kingdom of all these Bugs/Diseases/Viruses... Not one plant duded out and everything was super dank... again next round...same room... green crack... different treatments... 95% DUDS...

I noticed Fungus Gnats... and what I thought was a Katydid most likely a Leaf Hopper

lets hear from other people who have this problem...and not from naysayers who have zero experience with this issue

Well I have had zero experience with these issues with my own work, but I have seen it in other peoples grows.
I am happy to say nay when I feel it is called for, and am wondering how many of your posts have anything to do with Cannabis? Until we find out, go ahead and keep them coming but maybe label them "MAYBE IN CANNABIS"?
I also agree the real solution is to find labs that can test living plants for pathogens, in each state where legal to do so.
No one knows of any labs that do such testing? Unbelievable!!!
America is so backwards.....
-SamS

 

[RetroGrow]

Well I have had zero experience with these issues with my own work, but I have seen it in other peoples grows.
I am happy to say nay when I feel it is called for, and am wondering how many of your posts have anything to do with Cannabis? Until we find out, go ahead and keep them coming but maybe label them "MAYBE IN CANNABIS"?
I also agree the real solution is to find labs that can test living plants for pathogens, in each state where legal to do so.
No one knows of any labs that do such testing? Unbelievable!!!
America is so backwards.....
-SamS

Well said. My thoughts exactly......

 

[mofeta]

California Department of Food and Agriculture Plant Pest Diagnostics Center - Plant Pathology Laboratory

These guys are top-tier plant pathologists with state of the art equipment. They page I link to above even mentions phytoplasma diagnosis.

I bet they would test cannabis samples. One of you Cali guys should email or call them.

I saw this picture on the web earlier:
LEAFHOPPERS

 

[Sam_Skunkman]

California Department of Food and Agriculture Plant Pest Diagnostics Center - Plant Pathology Laboratory

These guys are top-tier plant pathologists with state of the art equipment. They page I link to above even mentions phytoplasma diagnosis.

I bet they would test cannabis samples. One of you Cali guys should email or call them.

I saw this picture on the web earlier:
LEAFHOPPERS
View Image

Good idea,
I posted the links to CDFA PPCD-Plant Pathology Laboratories already in this thread it did not seem to help. Some one in Calif needs to do it, or at least try....
-SamS

 

[Elmer Bud]

G`day Sam

I found this recently .
Mr R C Clarke
Cannabis Hemp Pests and Diseases with R C Clarke. BS

Thanks for sharin

EB .

 

[gaiusmarius]

seems to be hard to get things lab tested? that's been suggested since page one. obviously it's the only way to know which symptoms are which exact problem. i guess until the right guy discovers the dud symptoms who also has lab test access we will have to keep reading what is available. plants are plants after all and many problems in the plant world are not species specific. if it's too much reading, or not on a topic you want to read through, just skip the damn post and don't waste space saying how it wasn't to your interest. it just isn't constructive. threads like this work best when people follow the old adage, if you ain't got nothing nice to say, say nothing. maybe exchange the word nice with constructive.

 

[Storm Shadow]

Any information on Arabidopsis thaliana Higher Plants is relevant to Cannabis

I have put together a pretty insane IPM strategy based on a lot of the information from those links I posted....

I'm not dissing anyone at all....but this is a new phenomenon and if you haven't experienced .... you have no base to write off anything...

new phenomenon

mofeta... thank you for all your feedback so far bro, I find it to be very informative

 

[Storm Shadow]

http://www.promegaconnections.com/plant-biologists-take-the-lead-on-elucidating-zombie-genetics/


May 5, 2014 by Michele Arduengo
Plant Biologists Take the Lead on Elucidating Zombie Genetics

Biology is full of stories that read like a modern day zombie apocalypse. For instance, the parasite Toxoplasma gondii has been in the news for its ability to infect the brains of rats, and reprogram their normal behavioral responses such that they lose their innate fear of cats. Previously, we reviewed the research about the parasitic fungus that infects ants, causing drastic changes in typical ant behavior to aid in distribution of the fungal spores.
In April of this year, MacLean and colleagues published research in PLOS Biology describing interactions between a phytoplasma parasite and Arabidopsis thaliana. What is nice about this particular “zombie” biology story is that the researchers present the beginnings of the genetics that underlie the plant-parasite-insect relationship, moving beyond a description of the phenotypic changes that occur to describing an actual mechanism for those changes.
Phytoplasmas are bacterial plant parasites with life cycles that require two distinct hosts, the plant and insects that feed on the vegetative structures of the plants. Phytoplasmas can effect dramatic developmental changes on plants that include converting flowers to leaf-like structures or causing a proliferation of stems known as a witches’ broom. In the study authored by Maclean, the researchers looked at the interactions among “Aster Yellows phytoplasma strain Witches’ Broom” (AY-WB), its insect vector (leafhoppers) and the wild brassica plant, Arabidopsis thaliana.
In Arabidopsis plants infected with AY-WB, flowers are transformed into leaf-like structures. The plant loses its ability to reproduce as a result, and exists solely to provide food for its phytoplasma parasite—essential becoming a “zombie” plant.
The patterning and development of the floral meristem involves an intricate sequence of gene regulation turning “on” or “off” the correct genes at the precise time in the precise place to produce the concentric whorls of flower structure: sepals, petals, stamens and carpels. In previous work, this team had identified a protein from AY-WB, SAP54, which is required for this transformation and showed that Arabidopsis plants transformed with this protein develop the same leaf-like structures seen in the infected plants.
In this paper, the researchers began by asking what Arabidopsis proteins interact with SAP54. A yeast two-hybrid screen identified Type II MADS-domain containing transcription factors (MTFs). Members of this class of proteins include AGAMOUS-LIKE 12, MADS AFFECTING FLOWERING1 and several other transcription factors that specifically regulate genes that are responsible for the conversion of vegetative to flowering meristem and the production of the flower structure. Further investigation revealed that the interaction involved the keratin-like domain of these proteins, which is unique to flowering plants and not found in the MTFs of other organisms such as the leaf hopper.
They next looked at the interactions between SAP54 and Arabidopsis proteins in infected plants using GFP tagged SAP54 transgenic plants. Immunoprecipitation and mass spectrometry analysis revealed that many of the MTFs identified in the yeast two-hybrid screen do indeed interact with SAP54 in plants.
The researchers hypothesized that SAP54 may be interfering with MTF function. Analysis of Western blots from healthy and infected plants expressing tagged MTFs indicated that MTF proteins are less abundant in the leaf-like structures of the infected plants. These and other experiments led the team to propose that SAP54 is affecting the stability of the MTF proteins either directly or by upregulating their degradation using a pathway such as the ubiquitin system. Because the treatment of samples with epoxomicin, an inhibitor of the ubiquitin degradation pathway, allowed the Type II MTFs to accumulate, the researchers concluded that SAP54 was promoting the destabilization and degradation of the Type II MTFs using the hosts normal ubiquitin tagging and protein degradation pathways.
The two-hybrid screen used to identify the interaction of Type II MTFs with SAP54, also revealed interaction between SAP54 and RAD23C and RAD23D. These proteins are believed to be proteins responsible for shuttling ubiquitin-tagged proteins to the proteasome for degradation. Thus SAP54 appears to create a “short-circuit” between two cellular pathways, flower development and protein degradation, by directly interacting with key proteins in both pathways.

But that’s not all.
The production of leaf-like structures instead of flowers has the advantage of making the plants more “attractive” to the leaf hopper insect vectors. These insects feed on the vegetative tissues of the plants, and the phytoplasma are spread from plant to plant when a leaf hopper feeds on an infected plant and then moves to another plant. The researchers performed leaf hopper “choice assays” and found that the insects were more likely to colonize both the infected plants and transgenic plants expressing SAP54, than wildtype plants or plants that produced green flowers as a result of an unrelated mutation. Furthermore this preference appears to be RAD23 dependent, although the exact nature of the RAD23 role is not understood.
This research showed how a small, bacterial parasite uses one protein to change the developmental program of a plant, converting the plant into a vegetative food-producing system on which that parasite’s vectors are more likely to lay eggs and feed. Amazing. Zombie Plant Genetics—Just another reason I love science.

 

[Storm Shadow]

Phytoplasmas and Phytoplasma Diseases: A​

Severe Threat to Agriculture