in that example, one has already reversed 5 individual females. no reason not to take 5 clones from each individual female and produce fem ICs alongside the S2s. this would increase the lines from 5 to 25, and also help allow one to perform linkage analysis on the 5 parents. i do not disagree, just believe it does not go far enough. no need to go to s3 to determine homozygosity w/i a line's population if one can IC and BC and collect phenotypic data from the progeny to determine likely parental genotypic values. the end goal is the same; to determine which lines to combine to promote hybrid vigour while retaining the desired phenotypic traits. the only difference is in his example it takes 3 breeding seasons to determine and produce the desired line cross, while in mine i am popping and testing the desired line after the first season, although i do not know it until they are grown out and all the data collected and analyzed. stay frosty,
-iD
-iD