To all,
just had a nice response, then tried to send and deleted it...
long story short...
as part of a "Fun Project" after grad school, I and some friends ran 55 samples from the Pan Pacific Area...
this was able to be done as the samples were collected via old classmates in post-doc programs at UH, USC and UC...
we produced tissue cultures, used commercial kits to extract DNA... extracted both Nuclear and mitachondrial DNA...project languished a bit in the lab...finally developed primers to target specific stranded DNA to be sequenced ..."primed" for replication with a short complementary strand at one end. This preparation was divided into four batches, and each treated with a different replication-halting nucleotide, together with the four "usual" nucleotides. Each replication reaction then proceeds until a reaction-terminating nucleotide is incorporated into the growing strand, whereupon replication stops. Thus, reaction produces new strands that terminate at positions corresponding to the G's in the strand being sequenced. (Note that when long strands are being sequenced the concentration of the reaction-terminating nucleotide must be carefully chosen, so that a "normal" C is usually paired with a G; otherwise, replication would typically stop with the first or second G.) We then used Gel electrophoresis -- one lane per reaction mixture and then separated the replication products, from which the sequence of the original single strand can be inferred.
Results:
of the 55 samples 23 were sucessfull...from the chromatagraphs
the following inferences were made:
2 samples from the Hawaiian sampling were mitachondrial duplicates... the 100% sativa from the island of Molokai called The Blood, and one of the 6 samples called Maui Wowie from the Island of Maui...what was interesting is that 2 samples from california... 1 called "maui wowie" from a source in Ontario and a second from Santa Cruz were also sisters and all related to a mother common as our two Hawaiian samples. The Santa Cruz sample was called "Cherry Bomb".
At some point in a review of the data by the co contributors...some two years after the original culturing took place the name "Mr.Greengenes" came up as the source of the Santa Cruz sample.
Keep in mind that certain chain of custody issues as well as timing and the very " jovial' nature of the sampling would render it useless by peer review, none the less... I guess I stand by the work...
Why not contact RMBC and pose the question to them... I myself have aquired CB2, and if I have time and inclination( and quiet days with the Lycor) I may try the samples again...the primers are a real pain to develop however...
but I will keep my eyes open for that Sativa pheno...@ $35.00 US for 12 seed how can you go wron.
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