maharaji
Member
![Thank You :thank you: :thank you:](/data/assets/smilies/thankyou.gif)
Secondtry if you ever come across an OPT cut you'll be a very lucky man
Cheers guys!
+1
And I really agree with this: "I really like the idea of tetraploidy, it really can expand the plant we know today".
I thought about selling the seeds I will make (it will take me about 1-2 years to make the final version) but I will for sure send tons of freebees out to you guys! I wouldn't mind making a bunch of money for all my effort and time and studying tho!
First I will cross a female Thai 4n by a (masculinezed [sic]) female Nepalese 4n. I will take that F1 and self it to the S3 to S5 (selfed generations). Then I will cross the female (Thai 4n x Nepalise 4n) with a (masculinezed [sic]) female Afghan 4n. I will take that F1 and self it to S3 to S5 (depending upon when I see stabilization of traits); I won't go past S5. Once I have the S3 to S5 all female (Thai 4n x Nepalese 4n) x Afghan 4n I will give the seeds away and maybe sell them considering my variety should be WAY better thna most all others in terms of cannabinoids and yield...and much more unique (colors, bud and leaf size, seed size and color, etc).
Anyone want to suggest a name for the variety to be???
When I make tetraploids I will treat seeds before the shell cracks, I plan to soak seeds in water for 10 hours, then soak in the mutagen/water for 14-20 hours (until seed splits) then I will put in media to sprout.
Didn't you mean "if & when" you make tetraploids?
That outcome is by no means certain, or predictable.
However, you're on the right track by pre-soaking the seeds.
Since the moisture content of seeds is variable, don't soak for any set time. Let them soak, and stir occasionally, until they sink or suspend in 3 or 4 inches of RO water. Then let them sit for 20 more more minutes, remove and soak in the mutagen treated water.
When it works, you'll see a bulbous toot tip. The little sucker stands out like a sore thumb. Many will die, and most won't get much farther than the root tip stage. The sprouts first leaves are weird and slow growing. Most never get a 2nd set ... they just stay runts.
Using "educational grade" Colchicine helped, but only 1 in 50 grew out, and it really wasn't worth the trouble.
Good luck.
Nope. I believe in positive thinking, so what i wrote is what I meant. And the outcome is predictable, polyploid cannabis is not hard at all to make, look at the thesis paper, HortraPharm BV, etc, etc, etc.BassA wrote:
Didn't you mean "if & when" you make tetraploids?
That outcome is by no means certain, or predictable.
Personnaly my only concern with possible Dj mutated genes would be a health issue , but If you don't think that's the case with F13 secondtry then i'm reassured as you seem to have done your homeworks twice ( i checked your LED link too, really nice)
Cheers!
On another note, I picked up some bud last after speaking of Sativas. Super energetic high if you would like to incorporate that in to your mix i think it would prove useful. Its either a highland Jamaican or a colombian. PM me for details.
I'll PM you shortly, but the Thai I plan to use is the the Seedsman "Mama Thai" (Link). the Nepalese is the pure "charas" sativa from World of Seeds "Nepalese Highland" (Link) and the Afghan is from Seedsman "Original Afghani #1" (Link).
Thanks! I really can't wait to get started on this, but I need more room!![]()
Quote secondtry: "I really can't wait to get started on this, but I need more room!"
This is the biggest issue I face in the game of breeding,wouldn't it be nice to have unlimited space to conduct all the breeding experiments you could think of. Some people do!
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