Cytokinin

[h.h.]

J. van OVERBEEK (Rijksuniversiteit Utrecht) introduced in 1941 coconut milk as a new component of nutrient media for callus cultures. Coconut milk is liquid endosperm. In nature does it stimulate the embryo to grow which it supplies at the same time with food. Results yielded from callus cultures showed that its active components stimulate the growth of foreign cells, too.

In 1954 developed F. SKOOG (University of Wisconsin, Madison) a technique for the generation and culture of wound tumour tissue from isolated shoot parts of tobacco (Nicotiana tabacum). The thus developing callus grows when supplied with yeast extract, coconut milk or old DNA preparations. Freshly prepared DNA has no effect but becomes effective after autoclaving. This led to the conclusion that one of its breakdown products is required for cell growth and division. The substance was characterized. It is called kinetin and has been classified as a phytohormone.

http://www.biologie.uni-hamburg.de/b-online/e29/29a.htm#04
Aerating the milk with a handful of ewc, some molasses, some decomposed joshua tree heart, and Home Depot's version of e.m.(Kelloggs). Trying to combat the potassium molybisulfite in the milk. On the bright side p.m. is used to dissipate chloromine. I doubt if there is very much once diluted anyway

 

[grapeman]

Just for your info. Using Pro Gibb Plus has benefits,

http://www.valent.com/Data/Labels/20...004-5005r4.pdf

it’s so safe you can eat the stuff and registered and used on organic crops worldwide. You can acquire it on eBay for less or same as the other products mentioned.

The Pro Gibb Plus comes in a 160 gram bottle in powdered form.

To make a one (1) gallon solution of 100 ppm concentration (the strength required to do what you want to do) is as follows;

1 gallon H2O
2 grams of Pro Gibb Plus powder.

Resulting concentration is exactly 101.5ppm

That give you 80 mixes of 1 gallon spray. Can’t get any more economical than this.

Or if you make ½ gallon mixes and use 1 gram you would get 160 spray solutions. Even better.

 

[rrog]

Bad link.

Gibberilic Acid is not harmless as far as I'm concerned. First, it turns females into males from what I can gather, and second, you are warned not to smoke treated plants.

 

[h.h.]

They warned me of a lot of things...Sometimes it does pay to listen. Not that I did.
To explain my tea a little.
As joshua tree deteriorates, termites and other bugs burrow into the heart leaving a very fibrous black material with a very sweet earthy smell. The desert's version of ewc. In a 1 gallon jar I added 1 can of cc milk, a little blackstrap, the jt heart, ewc,em, and now some ant hill dirt. A yogurt container, 1/2 full of char, plugs in the top. A styro cup, with a seedling, plugged into a hole in the container's lid. The container and the cup have covered holes in the bottom to let liquid and air through. It is filled to the brim of the gallon jug with water.
The foam covers the char and wets the cup. The char gets coated with all this creamy glob of tasty looking microbe food while the seedling is getting nourished.

 

[h.h.]

A quick sketch. I added some banana to the air space around the cup trying to capture some ethylene.

 

[h.h.]

In the last post.
The first pic is a different version of this and the second on is my version of the OBBT.

 

[h.h.]

A little Neil Young to fill a screwed up post...
http://www.youtube.com/watch?v=n7ZkQC0riwc

 

[h.h.]

http://www.biologie.uni-hamburg.de/b-online/e29/29a.htm#04
Aerating the milk with a handful of ewc, some molasses, some decomposed joshua tree heart, and Home Depot's version of e.m.(Kelloggs). Trying to combat the potassium molybisulfite in the milk. On the bright side p.m. is used to dissipate chloromine. I doubt if there is very much once diluted anyway

After bubbling for two days all while feeding the seedlings and capturing the foam with the char the coconut tea was added to my buckets and the seedling cup placed on top. Still "fresh" after 6 days. Signs of fungus make me think the sulfite was not a problem.
The smallest seedling received the coconut tea.
The largest received tea and banana treatment. A third seedling started 2 days later, with no coconut or banana treatments, is catching up fast to the large one.
Each set up is a bit different so it's not real scientific. I've started to rotate the cups between buckets to see if similarities occur in the seedlings. Like they all die or something

 

[rrog]

What's the banana treatment?

 

[h.h.]

The seedling is in a bottomless styro cup sitting in a cutout yogurt container. Banana is stuffed alongside the cup where theoretically the ethylene is carried with the oxygen to the roots with any escaping going to the upper plant zone.

 

[rrog]

Got it, thanks. So for Ethylene.

 

[h.h.]

It's hard to tell if the bananas are having much effect as far as growth..
The seedling treated with the most cc milk will probably give up today. It was the one that showed the early fungus as well. Pulling out the filter it was in showed it was coated with the fat from the milk. Not knowing what part of the milk separation that holds the CK, I did this somewhat intentionally. I doubt if much got through the filter though. There may well be some other cause. I'll have to repeat the trial.
That was one can of milk in one gallon of tea, bubbled through char, and directed at a styro cup full of medium. In my larger buckets, I have about 4 gallons of water and probably 3 gallons of medium with a can and a half of milk. The seedlings in those are just perking away.

 

[rrog]

Personally, I'm done with Ethylene. I'm no longer interested in feminized males.

The Cytokinin, however, is a different story. I had all females in my last (and only) grow, and the Cytokinin (used Nitrozyme) really packed on bud weight.

 

[h.h.]

Legally I can only keep 6 out of 12 plants so I'm better off culling the males anyway.
As for the bananas a trace of ethylene or whatever they contribute isn't hurting to say the least.
The cc milk is working in the buckets. At least one. The other had a design problem and was getting poor circulation as well as letting fines settle and go bad. Nothing to do with the milk. The second contains 1.5 cans with only a earthy sweet smell.

 

[rrog]

I saw data that said apples were higher in Ethylene production than bananas. Maybe, maybe not. I used both for 2 weeks this last time.

Seedlings grew stems to the point where one fell over on itself. Clearly stem and root growth are affected by Ethylene. I did it too much in my estimation. 2 of three seedlings made it, and are doing very well, but the third is being replaced.

So too much can be a negative from my perspective.

 

[h.h.]

Now we know. Well at least have an idea. Thanks for the results either way.

 

[rrog]

I'm with you. If no one does these things and communicates them, how do we move forward as a group. I look at my failures as something learned.

 

[grapeman]

Bad link.

Gibberilic Acid is not harmless as far as I'm concerned. First, it turns females into males from what I can gather, and second, you are warned not to smoke treated plants.

well rrog, sorry to say you are terribly mis-informed about gibb. it is an auxin that is naturally made by all green plants and in all foods you eat in some concentration.

It is so safe and natural that is registered for use in organic farming. It's ignorance like yours that is leading us to cap & trade and free health care for all, which will crater the country. But I learned long ago that there is no common sense that gets through to people who treat organic food and farming like a religion.

 

[h.h.]

Must have eaten some bad grapes.
If there is an error correct it just spare the lecture. Nobody cares.

 

[rrog]

Grape, there are so many sites that suggest not smoking Gibberillin treated seeds or plants. Maybe mass hysteria?

The beauty is that you are free to smoke any treated plant you like.

Clearly based on your caustic ignorant tone you're already aware of your right of free speech.

h.h. is right... spare the lecture. It's profoundly misplaced.