Tetraploid and triploid seeds

[gonanchoa]

Thanks! I found him...

It's something many people bf tried using mixoploids. Triploid watermelon doesn't yield seeds fex. But it didnt work on cannabis bc females would get full of empty white seeds! A drawback as a grower is that you need to buy seeds each season.

Good luck and keep us posted!

 

[RingtailCanyon]

Mac1 is a polyploid

 

[Piff_cat]

Here's the thing about Colombia and Sam's "3 way" hypothesis " considering he wasn't there in 69 for breeding. It can be true and false at the same time. Hazes wild progenitors are type 4 cannabis from east Asia. Or I should say nevils 69 hazes progenitors are from se Asian. Lynch genotypes by sequencing study in 2016 scientifically proves it. That doesn't mean the line didn't take a pit stop in Colombia first . All of the areas type 4 grows naturally are close to the pacific side of south America. There's a human haplotype group found on 2 places the ainu/joking and Ecuador. The ancient jade routes from Taiwan to North Vietnam down to phillipines and on to borneo and Indonesia problem spread the ancient chinese- Korea and Japanese cultivars for the first time. These countries have proof of cannabis domestication going back 12000 years starting with the Jamon Xinjiang ughyurs Swinton Korean Taiwan animism.. cultivars were selected for ancestors worship and their high lignin content creates a thick white smoke full of aromatic ethers. Smoke was not only for effect but also as a canvas for the visions. Chinese were using them as anesthia in some of oldest records of surgery type medicine. There's an old quote about necromancers mixing ginger and magic cannabis to see the future or backwards to the past. These plants are giant with square stems densely packed trichomes and fireman's ladder looking stem meriatem morphology. They have hollow stems- high cbg cbc and cannabigerol monomethyl ether. They utilize oxygenated terpenes as intermediary modified by phytochrome p450 enzymes which create ketones aldehydes esters ethers all of which contribute to an entourage effect. Many ethers are hallucinenic. The linalyl cation product in these genetics are normally the fuel for these aromas. Either transformed to ocimene/mycene or a cyclic monoterpenols. Cultivars have most cbc by far. When Lynch did his gbs testing it tracks snps synthesis etc. A second nld /sativa Claude was found besides Indian ganja. Every single one of the hybrids in this group were progeny on nevils haze males besides the thai/lao landraces. It's a very exciting break thru.

 

[RingtailCanyon]

Type 4 is all CBG, correct? And don’t type 4 phenos have the synthase for thc “turned off”
from HOku…
While types I, II, and III are fairly straightforward to breed, things get more complicated as you dive into type IV’s and beyond. According to OregonCBD, there is evidence of three specific routes to create type IVs. The first is a double deletion of the THCAS and CBDAS synthases, such as in the case of Santhica 27. The second, is an abnormal “nonfunctional” CBDAS synthase in the presence of a normal nonfunctional THCAS synthase and third, an abnormal “nonfunctional” THCAS synthase in the presence of a normal nonfunctional CBDAS synthase. Other theoretical methods might include introducing exogenous forces to shutdown protein expression, such as gene silencing through a virus.

 

[Piff_cat]

so the understanding of cannabinoid profiles gets clearer every day. originally small described type 4 as north east asian strains with cannabigerol monomethyl ether present . from there yes they found 2 types of cbg only those with cbg and cbd and those with cbg and thc. this was when everyone still thought that cbd and thc were on the same locus with co dominant alleles. fast forward to now and they realize that each species has a particular synthase and catalytic rate for producing precursors and synthesizing them into cannabinoids. not all thc synthases are the same genetically or metabolically. same thing with cbg cbc etc. the big difference is they are not on the same locus its not a dichotomy. the thc and cbd synthases are on the same chromosome(6) and are most likely inherited in linkage groups but its no longer an either or situation. it now looks like type 2 cannabis does not exist its more of a polymorphic complex. a;; thc/no cbd - all cbd/no thc. with every iteration in the middle. now theyve even found that cbca acts as a precursor by cbt and some cbc may not even start as cbga.i think cbc and cbt are high in haze and the cbt will account for the heart facing euphoria and cbc for the longer effect and serotonin increase. they also recently showed that if you cross a type to type 3 you get segregation in the trait. meaning 4 chemotypes
) also interesting to note that a haze clone tested for thc synthases had 4 of them!! check out this article its great and very relevant to haze and cbg

https://www.medicinalgenomics.com/w...th-Chemical-Phenotype-Phytochemistry-2015.pdf

 

[Randy Lahey]

Out of interest, how does one identify tetraploidy?

With the naked eye, put a flashlight under a shade leaf and you will see tiny white dots "Stomata"...
2n looks very empty, 3n is more visible and 4n is incredibly visible.

After that it's just picking the vigorous one that has the flavor, structure and resistance you wanna keep. Find it....gauge it's polyploidization by doing this.......isolate it..... Take clones.....keep it alive....."SELF" one of the clones....search your S1 seed for a Male....

"Variegated S1 Plants" ARE RECESSIVE and should NOT BE USED FOR BREEDING

Find your non Variegated S1 Male and pollenate a clone of the original keeper selected.
Then do it 2 more times. Searching for a male each time from the new seed and pollenating a clone of the original hybrid mother selected each time for 3x backcrossed seed stock of your new Heterozygous Cannabis Variety.

 

[goingrey]

Humboldt Seed Company has two triploid strains out: OG Triploid and Donutz Triploid

Sterile and 30%+ THC, so they claim.

 

[Alpenglow]

Humboldt Seed Company has two triploid strains out: OG Triploid and Donutz Triploid

Sterile and 30%+ THC, so they claim.

That went not so great i learned yesterday.
What the hell is happening with them?
They only sell a few regular strains these days in the US and Europe, looks like the time of the "Mega Pheno Hunts" are a thing of the past... sad times...

 

[goingrey]

That went not so great i learned yesterday.
What the hell is happening with them?
They only sell a few regular strains these days in the US and Europe, looks like the time of the "Mega Pheno Hunts" are a thing of the past... sad times...

Will have to listen to that video but it's quite long, what did you learn went wrong?

And if people aren't buying regs can't fault them for not offering them I suppose. A lot of companies that sell both have said that regs are like 1% of the sales. They have 56 feminized varieties on the US website, I think they're staying busy.

 

[Alpenglow]

Yes all right, but that they going for the Reg´s after the (promotional) pheno hunt is what made them outstanding to me.
The direction they went now with fem´s only and that sterile stuff is nothing for me.

 

[goingrey]

Well triploids will be sterile because of the odd chromosome count. No way around it. As far as I understand...

But I do agree, more regs would be cool.

 

[Alpenglow]

Yes, you are right on that

It´s a "another on bites the dust" thingy.
I really like them for the things they did in the past.
That Pheno Hunt was fun to watch, the interviews and podcasts too, the website and how they presented the strains, the expansion to europe, so i bought a couple strains from them to support it.
What they tell in that video above is pure bullshitting, cant agree with most of what they said there.
We will see how that turns out, but when i look at the decriminalisation in Germany and what most of those new growers have bought for their first grow (auto, auto-fem, fem, in that order) and all that equipment they use to control the environment makes me shaking my head.
The Marketing Blogs and Tutorials from Companys like Royal Queen Seeds work pretty well.
From my point of view, triploid is just the next step in that closed loop.
Kind of feeling old when i look at all that stuff that happened in the past weeks and of course years
We are privileged with all those Seeds in our fridge and for the way we learned how to grow.
It´s a new generation now with all that discord and instagram stuff.

 

[goingrey]

When fems came out people said they are ruining the genepool with them, well even before that when everything was crossed to Skunk #1 people said they are ruining the genepool... And with autos they said it especially.

Can't say that about triploids, them being sterile and all.

 

[Alpenglow]

And it is what it is today, lots of F1 hybrids, damage is done already.
Better i accept of being a modern hippie with my landraces and reg stuff in the fridge and take a step away from that rabbit hole of triploids

 

[CaptainDankness]

When fems came out people said they are ruining the genepool with them, well even before that when everything was crossed to Skunk #1 people said they are ruining the genepool... And with autos they said it especially.

Can't say that about triploids, them being sterile and all.

Triploids are horrible if they become popular. When fems started it was just Dutch Passion then everything at Greenhouse seeds and spread like wildfire.

If Triploids become popular the same thing will happen, everyone is going to want to triploid their plants and all the new genetics will be impossible to work except for the big seed companies in house. The reason we have variety of today is everyone making weird crosses, sometimes on accident. All these clone lines that come from an accidental hermaphrodite never going to happen again. No Girl Scout cookies, no GG#4 and these breeders have to get genetics from us, Greenhouse, Humboldt Seed Company, really everyone is coming to the streets to buy a clone of the next best cut that comes from a breeder or accident.

We will still have a lot of people selling regular seeds and breeding, just won't have as many unique plants coming out.

 

[goingrey]

Triploids are horrible if they become popular. When fems started it was just Dutch Passion then everything at Greenhouse seeds and spread like wildfire.

If Triploids become popular the same thing will happen, everyone is going to want to triploid their plants and all the new genetics will be impossible to work except for the big seed companies in house. The reason we have variety of today is everyone making weird crosses, sometimes on accident. All these clone lines that come from an accidental hermaphrodite never going to happen again. No Girl Scout cookies, no GG#4 and these breeders have to get genetics from us, Greenhouse, Humboldt Seed Company, really everyone is coming to the streets to buy a clone of the next best cut that comes from a breeder or accident.

We will still have a lot of people selling regular seeds and breeding, just won't have as many unique plants coming out.

Depending on how legalization pans out we may see both small timers and big seed companies being able to grow large numbers and actually purposefully breed interesting and unique plants and we won't have to rely on hermie bagseed for them.

 

[Alpenglow]

Everybody will stop growing and just buy weed, yes?

 

[goingrey]

Everybody will stop growing and just buy weed, yes?

The demographics and methods will change but growing will just become more popular.

I see a future of a lot of people growing auto fem triploids on their windowsills.

 

[Alpenglow]

If it becomes legal, you could just buy the cut or the weed, no triploid seeds needed.
I still dont see the point why stuff like this is a good thing.
Auto fem triploid seeds... im dying inside

 

[CaptainDankness]

Depending on how legalization pans out we may see both small timers and big seed companies being able to grow large numbers and actually purposefully breed interesting and unique plants and we won't have to rely on hermie bagseed for them.

They definitely won't be letting small timers grow large numbers, it's going to be regulated worse than anything else. Now that it's going to be schedule 3 we're probably going to have to get licensed by the DEA to grow it and that's only going to be the big guys.

The demographics and methods will change but growing will just become more popular.

I see a future of a lot of people growing auto fem triploids on their windowsills.

Definitely no need for auto fem triploids. Lol, I see a future of growing weed like outlaws back in the day, only a hell of a lot more government spying. Schedule 3 is terrible Big pharma is going to throw out all kinds of propaganda and all their lobbyists pushing to approve their "safer, quality controlled, perfectly dosed cannabinoid drugs" They will have hundreds of studies on how certain cannabinoids could be harmful and they're our only protection from the dirty compounds in the devil's lettuce. Lol