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Tetraploid and triploid seeds

gonanchoa

Active member
Thanks! I found him...

It's something many people bf tried using mixoploids. Triploid watermelon doesn't yield seeds fex. But it didnt work on cannabis bc females would get full of empty white seeds! A drawback as a grower is that you need to buy seeds each season.

Good luck and keep us posted!
 

Piff_cat

Well-known member
Here's the thing about Colombia and Sam's "3 way" hypothesis " considering he wasn't there in 69 for breeding. It can be true and false at the same time. Hazes wild progenitors are type 4 cannabis from east Asia. Or I should say nevils 69 hazes progenitors are from se Asian. Lynch genotypes by sequencing study in 2016 scientifically proves it. That doesn't mean the line didn't take a pit stop in Colombia first . All of the areas type 4 grows naturally are close to the pacific side of south America. There's a human haplotype group found on 2 places the ainu/joking and Ecuador. The ancient jade routes from Taiwan to North Vietnam down to phillipines and on to borneo and Indonesia problem spread the ancient chinese- Korea and Japanese cultivars for the first time. These countries have proof of cannabis domestication going back 12000 years starting with the Jamon Xinjiang ughyurs Swinton Korean Taiwan animism.. cultivars were selected for ancestors worship and their high lignin content creates a thick white smoke full of aromatic ethers. Smoke was not only for effect but also as a canvas for the visions. Chinese were using them as anesthia in some of oldest records of surgery type medicine. There's an old quote about necromancers mixing ginger and magic cannabis to see the future or backwards to the past. These plants are giant with square stems densely packed trichomes and fireman's ladder looking stem meriatem morphology. They have hollow stems- high cbg cbc and cannabigerol monomethyl ether. They utilize oxygenated terpenes as intermediary modified by phytochrome p450 enzymes which create ketones aldehydes esters ethers all of which contribute to an entourage effect. Many ethers are hallucinenic. The linalyl cation product in these genetics are normally the fuel for these aromas. Either transformed to ocimene/mycene or a cyclic monoterpenols. Cultivars have most cbc by far. When Lynch did his gbs testing it tracks snps synthesis etc. A second nld /sativa Claude was found besides Indian ganja. Every single one of the hybrids in this group were progeny on nevils haze males besides the thai/lao landraces. It's a very exciting break thru.
 

RingtailCanyon

Well-known member
Type 4 is all CBG, correct? And don’t type 4 phenos have the synthase for thc “turned off”
from HOku…
While types I, II, and III are fairly straightforward to breed, things get more complicated as you dive into type IV’s and beyond. According to OregonCBD, there is evidence of three specific routes to create type IVs. The first is a double deletion of the THCAS and CBDAS synthases, such as in the case of Santhica 27. The second, is an abnormal “nonfunctional” CBDAS synthase in the presence of a normal nonfunctional THCAS synthase and third, an abnormal “nonfunctional” THCAS synthase in the presence of a normal nonfunctional CBDAS synthase. Other theoretical methods might include introducing exogenous forces to shutdown protein expression, such as gene silencing through a virus.
 

Piff_cat

Well-known member
so the understanding of cannabinoid profiles gets clearer every day. originally small described type 4 as north east asian strains with cannabigerol monomethyl ether present . from there yes they found 2 types of cbg only those with cbg and cbd and those with cbg and thc. this was when everyone still thought that cbd and thc were on the same locus with co dominant alleles. fast forward to now and they realize that each species has a particular synthase and catalytic rate for producing precursors and synthesizing them into cannabinoids. not all thc synthases are the same genetically or metabolically. same thing with cbg cbc etc. the big difference is they are not on the same locus its not a dichotomy. the thc and cbd synthases are on the same chromosome(6) and are most likely inherited in linkage groups but its no longer an either or situation. it now looks like type 2 cannabis does not exist its more of a polymorphic complex. a;; thc/no cbd - all cbd/no thc. with every iteration in the middle. now theyve even found that cbca acts as a precursor by cbt and some cbc may not even start as cbga.i think cbc and cbt are high in haze and the cbt will account for the heart facing euphoria and cbc for the longer effect and serotonin increase. they also recently showed that if you cross a type to type 3 you get segregation in the trait. meaning 4 chemotypes
) also interesting to note that a haze clone tested for thc synthases had 4 of them!! check out this article its great and very relevant to haze and cbg

https://www.medicinalgenomics.com/w...th-Chemical-Phenotype-Phytochemistry-2015.pdf
 

Randy Lahey

Active member
Out of interest, how does one identify tetraploidy?


With the naked eye, put a flashlight under a shade leaf and you will see tiny white dots "Stomata"...
2n looks very empty, 3n is more visible and 4n is incredibly visible.

20230128_212518[1].jpg


20230128_212820[1].jpg



After that it's just picking the vigorous one that has the flavor, structure and resistance you wanna keep. Find it....gauge it's polyploidization by doing this.......isolate it..... Take clones.....keep it alive....."SELF" one of the clones....search your S1 seed for a Male....

"Variegated S1 Plants" ARE RECESSIVE and should NOT BE USED FOR BREEDING

20220822_073720[1].jpg


Find your non Variegated S1 Male and pollenate a clone of the original keeper selected.
Then do it 2 more times. Searching for a male each time from the new seed and pollenating a clone of the original hybrid mother selected each time for 3x backcrossed seed stock of your new Heterozygous Cannabis Variety.
 
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goingrey

Well-known member
Humboldt Seed Company has two triploid strains out: OG Triploid and Donutz Triploid

Sterile and 30%+ THC, so they claim.
 
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