Male clones transformed to Female to judge male smoking quality - Breeder's Laboratory - International Cannagraphic Magazine Forums

**Sam_Skunkman** · 09-18-2008, 08:11 AM

Has anyone done this?

-SamS

Fertile female flowers can be induced in male plants by ethephon (2-chloroethanephosphonic acid) and NIA 10637 (ethylhydrogen-l-propylphosphonate). Interestingly, stamens could be seen arising even from fruits. Stopping the application of growth regulators caused the plants to revert to their original sex. We hypothesized that in Cannabis, GA and ethylene act as male and female hormones respectively, and that the expression of sex is controlled by a balance between their endogenous levels. Abscisic acid (ABA) is able to overcome the GA induced male flower formation (Mohan Ram and Jaiswal 1973; Mohan Ram and Sett 1985).

Mohan Ram H Y and Sett R 1985 Cannabis sativa; in CRC
handbook of flowering (ed. Halevy A H) (Boca Raton: CRC
Press) Vol. II, pp 131–139

Mohan Ram H Y and Jaiswal V S 1973 The possible role of
ethylene and gibberellins in flower sex expression of Canna-
bis sativa; in Proceedings of 8th International Conference on
Plant Growth Substances (Tokyo: Hirokawa Publishing Co)
pp 987–996172

Although many environmental groups worry about toxicity resulting from use of growth hormones and fertilizers, the toxicity of ethephon is actually very low, and any ethephon used on the plant material is converted very quickly to ethylene.

dubi · 09-18-2008, 08:20 AM

Good day Sam,

I have reversed a few females with STS but never did it with a male to check her hidden female expression and traits.

After STS application, silver is stored in the plant in toxic quantities.... reversing a male into female you can check some important flowering traits like aromas, resin production, ovary and pistil formation/distribution but have you found a non toxic reversal product so you can smoke your reversed males?

**Sam_Skunkman** · 09-18-2008, 08:40 AM

As far as I know ethephon is pretty harmless...

-SamS

dubi · 09-18-2008, 08:53 AM

Thanks Sam,

My chemist knwoledge is nule but i was curious about non toxic alternatives to STS. Have you smoked reversed males treated with ethephon?

esbe · 09-18-2008, 08:55 AM

good idea! must be fun to try!

**Sam_Skunkman** · 09-18-2008, 08:56 AM

No I have not.

-SamS

melvin2 · 09-18-2008, 08:59 AM

I was just thinking about this today. Funny to see a post on the same day. Like would a male show it's traits through female flowers, or would recessive female genes come out during a sex change?

dubi · 09-18-2008, 08:59 AM

Yes, esbe im curious to try it too but im worried about toxicity....

esbe · 09-18-2008, 09:05 AM

otherwise you must try the "naturel" stress methods or get the buds tested on laboratory

JWP · 09-18-2008, 09:20 AM

Sounds like a good idea. Take cuts from all the males. Reverse the plant to see which has the best female quallities and use the best cuts to pollinate the prised female.
Produce some uber beans
Just doubled the frequency of the genes resposable for the traits you want. The term should be called "Stacking" ... lol ... stacking dominant traits on alleles

09-18-2008, 08:11 AM	#1
Sam_Skunkman "RESIN BREEDER" Join Date: Mar 2004 Posts: 4,660	Male clones transformed to Female to judge male smoking qualities Has anyone done this? -SamS Fertile female flowers can be induced in male plants by ethephon (2-chloroethanephosphonic acid) and NIA 10637 (ethylhydrogen-l-propylphosphonate). Interestingly, stamens could be seen arising even from fruits. Stopping the application of growth regulators caused the plants to revert to their original sex. We hypothesized that in Cannabis, GA and ethylene act as male and female hormones respectively, and that the expression of sex is controlled by a balance between their endogenous levels. Abscisic acid (ABA) is able to overcome the GA induced male flower formation (Mohan Ram and Jaiswal 1973; Mohan Ram and Sett 1985). Mohan Ram H Y and Sett R 1985 Cannabis sativa; in CRC handbook of flowering (ed. Halevy A H) (Boca Raton: CRC Press) Vol. II, pp 131–139 Mohan Ram H Y and Jaiswal V S 1973 The possible role of ethylene and gibberellins in flower sex expression of Canna- bis sativa; in Proceedings of 8th International Conference on Plant Growth Substances (Tokyo: Hirokawa Publishing Co) pp 987–996172 Although many environmental groups worry about toxicity resulting from use of growth hormones and fertilizers, the toxicity of ethephon is actually very low, and any ethephon used on the plant material is converted very quickly to ethylene. Last edited by Sam_Skunkman; 09-18-2008 at 05:13 PM.. Reason: correction
	Quote 10 members found this post helpful.

09-18-2008, 08:20 AM	#2
dubi Vendor Join Date: Apr 2006 Posts: 6,891	Good day Sam, I have reversed a few females with STS but never did it with a male to check her hidden female expression and traits. After STS application, silver is stored in the plant in toxic quantities.... reversing a male into female you can check some important flowering traits like aromas, resin production, ovary and pistil formation/distribution but have you found a non toxic reversal product so you can smoke your reversed males?
	Quote 4 members found this post helpful.

09-18-2008, 08:40 AM	#3
Sam_Skunkman "RESIN BREEDER" Join Date: Mar 2004 Posts: 4,660	As far as I know ethephon is pretty harmless... -SamS
	Quote 1 members found this post helpful.

09-18-2008, 08:53 AM	#4
dubi Vendor Join Date: Apr 2006 Posts: 6,891	Thanks Sam, My chemist knwoledge is nule but i was curious about non toxic alternatives to STS. Have you smoked reversed males treated with ethephon?
	Quote 1 members found this post helpful.

09-18-2008, 08:55 AM	#5
esbe ĦɎɃ®ɨɗ Ƒ®Øɱ Ħ£ɭɭ Join Date: Feb 2006 Location: good is everywhere Posts: 8,483	good idea! must be fun to try! __________________ .^ĦɎɃ®ɨɗS Ƒ®Øɱ Ħ£ɭɭ Click the gif to learn much more about HFH:
	Quote

09-18-2008, 08:56 AM	#6
Sam_Skunkman "RESIN BREEDER" Join Date: Mar 2004 Posts: 4,660	No I have not. -SamS
	Quote

09-18-2008, 08:59 AM	#7
melvin2 Member Join Date: Mar 2005 Posts: 232	I was just thinking about this today. Funny to see a post on the same day. Like would a male show it's traits through female flowers, or would recessive female genes come out during a sex change?
	Quote

09-18-2008, 09:05 AM	#9
esbe ĦɎɃ®ɨɗ Ƒ®Øɱ Ħ£ɭɭ Join Date: Feb 2006 Location: good is everywhere Posts: 8,483	otherwise you must try the "naturel" stress methods or get the buds tested on laboratory __________________ .^ĦɎɃ®ɨɗS Ƒ®Øɱ Ħ£ɭɭ Click the gif to learn much more about HFH: Last edited by esbe; 09-18-2008 at 09:07 AM..
	Quote

09-18-2008, 09:20 AM	#10
JWP Banned Join Date: Mar 2006 Posts: 1,000	Sounds like a good idea. Take cuts from all the males. Reverse the plant to see which has the best female quallities and use the best cuts to pollinate the prised female. Produce some uber beans Just doubled the frequency of the genes resposable for the traits you want. The term should be called "Stacking" ... lol ... stacking dominant traits on alleles
	Quote 1 members found this post helpful.