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#71
Old 03-30-2018, 10:48 PM
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In the future I think they will be able to identify specific genetic markers on Colombians, Thais, Africans etc. varieties.

but they started with the basics.

I think it will be a long slow process to work it all out if its anything like the work being done to sort out different Human groups and their origins.



"A genetic marker is a gene or DNA sequence with a known location on a chromosome that can be used to identify individuals or species. It can be described as a variation (which may arise due to mutation or alteration in the genomic loci) that can be observed. A genetic marker may be a short DNA sequence, such as a sequence surrounding a single base-pair change (single nucleotide polymorphism, SNP), or a long one, like minisatellites."
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#72
Old 03-31-2018, 01:50 PM
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Hi satva. I agree with you.

What I wanted to say is that those groups made by Phylos as skunk, berry, landrace, og kush, hemp and CBD are a bit strange.

My intepretation is that landrace = NLD.
I think hemp = European fiber hemp, so Cannabis sativa (NLH). I have no idea about CBD means, because NLH main cannabinoid usually is CBD.

About skunk, berry, and ok kush, all of them are NLD/BLD hybrids, so they carry mixed SNP's from their ancestors. They have some NLD and BLD SNP's.

As you have said, and I am completely agree with you, a 5-10% or so of berry or skunk in a NLD landrace must not means that landrace is contaminated or admixtured with modern hybrids. What I think it means is that landrace shares those 5% SNP's with the NLD component in the berry or skunk. Like this one:

https://testing.phylosbioscience.com...otype/58m6n6wg

But if you find a 40 or 50% of Skunk, OG Kush or so SNP's in a supposed pure NLD, then I suspect that landrace is contaminated or hybridized with modern hybrids for sure. For example like this:

https://testing.phylosbioscience.com...otype/5gkvz3o2

The fact is I don't know exactly what means the groups made by Phylos. Because a pure Afghan BLD is also a "landrace" too. And I don't know if berry means actually DJ Short's Blueberry.

About Acapulco Gold, really I don't know. I am too young and live too far to have known or smoked the real one.

I thought some time ago Mexican NLD's could be only carefully selected hemp (NLH) from Spain by Mexican farmers throughout a few centuries. Because some NLH strains in Southern Europe carry the BT alelle alongside the BD alelle.

https://onlinelibrary.wiley.com/doi/...2/ajb.91.6.966

Quote:
The presence of allele BT in the C. sativa gene pool suggests that introgression from C. indica might have played a role in the evolution of C. sativa. Wind-blown pollen may have contributed to allele migration between the two gene pools (Cabezudo et al., 1997⇓). Relatively high BT frequencies (range 0.38–0.55) were detected in seven hemp accessions from Turkey, Spain, Italy, former Yugoslavia, and southern Russia, which are assignable to the southern eco-geographical group of C. sativa (Davidyan, 1972⇓). Additional allozyme markers and morphological traits typical of C. indica were also observed in the southern group of C. sativa (Hillig, 2004⇓, in press).
Quote:
Human selection of plants carrying two copies of the BT allele appears only to be of appreciable significance in the domestication of the NLD biotype. Human selection may have resulted in an increase in the quantitative levels of cannabinoids produced by the WLD biotype, but the average amount of CBD + THC produced by the NLD biotype did not significantly differ from the hemp and feral biotypes of C. indica. In fact, the average amount of THC + CBD produced by the NLD accessions was not significantly greater than the average amount of these two cannabinoids produced by the hemp accessions of C. sativa. Small and Beckstead (1973b)⇓
Quote:
However, in some fiber ecotypes, like the one for which chemotypes distribution is shown in Figure 3 (an old Italian fiber ecotype, Eletta Campana), the number of plants that should be considered homozygous for THC was indeed not negligible; besides, the ab- solute amount of THC in the inflorescences, though not at the levels of the drug strains, is high enough to make this cultivar ineligible for EU subsidies. At the beginning of the 1990s, this situation was common for many dioecious fiber cultivars, and therefore the necessity arose, for an effective presence on the mar- ket, to “clean” the seed batches from THC-producing plants, without altering the overall genetic background.
https://www.icmag.com/ic/showpost.ph...&postcount=276

But later a good friend made me change my mind about that. Because it is more convincing Mexican NLD strains arrived there directly from South East Asia carried by the Manila Galleon. Which docked every year at the Acapulco harbour.

Mota: The historic role of Cannabis in Mexico

But I remembered my former hypothesis again when I saw this:

https://testing.phylosbioscience.com...otype/vgqqwq2g

Modern breeding is something too arrogant. I think no breeder can do in a few years the same work as farmers have done along centuries. But I don't know what could be the actual origin of the real old Acapulco Gold or Mexican NLD heirlooms or landraces.
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#73
Old 03-31-2018, 03:32 PM
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Edited: I found a Panama but I am not sure now if it is ACE Panama. It is very rare genetically.

https://testing.phylosbioscience.com...otype/mgj1z3g7








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Last edited by Natural high; 03-31-2018 at 03:50 PM..
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#74
Old 03-31-2018, 04:15 PM
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It's just me that I'm not convinced of the phylos?I'm not just talking about panama,in general,....
perhaps it also depends on who sends the sample ...
For example,panama is a 3 way,old panama,new panama and punto rojo,but phylos dont say that...
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#75
Old 03-31-2018, 05:33 PM
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Originally Posted by Natural high View Post
Edited: I found a Panama but I am not sure now if it is ACE Panama. It is very rare genetically.

https://testing.phylosbioscience.com...otype/mgj1z3g7

.
That looks like what I would expect from Panama red 1974. This is Ace panama, https://testing.phylosbioscience.com...otype/z810v1g7 showing surprisingly little western influence, depending on what that CBD component represents.

I can't be too convinced by Phylos either. We have to remember this is version number one. I'm sure in five or ten years they'l l get more sense into it. They should really consider using different reference groups instead of berry, skunk or what have you. Now it's like determining how much neanderthals had genes from modern New Yorkers.

I get it, growers today are ignorant of landraces so a name like OG Kush speaks to them a lot more than Acapulco Gold for example but maybe they should still consider taking a more scientific approach..

95% of seed bank strains today descend from 1. Original Haze 2. Skunk#1 3. Afghani 4. Northern Lights (which is a kind of afghan x skunk probably) Why not use those as your reference groups? Just add a hemp reference group and a few different landrace groups and you have it pretty much covered. CBD as a group doesn't mean anything at all.
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#76
Old 03-31-2018, 06:24 PM
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PANAMA ACE:

2 generations of Panama 74 x Panama Red Hair
1 generation of Panama 74 / Red Hair F2 x 3 males Panama Network of third line different
4 generations of this triad plus and voila (current seeds of Panama ACE for sale in regular and feminized)

https://www.cannabiogen.com/club/vie...=1653&start=30

Some descriptions include punto rojo but the ACE site has down as 3 panama strains

Anyone know the differences panama goddess and A22? thanks!


Ace version is very worked and developed and uses 1974 panama red standard regular seeds and fem

Greenthumb seems more wild and only feminzed
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#77
Old 03-31-2018, 08:54 PM
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Quote:
I can't be too convinced by Phylos either. We have to remember this is version number one. I'm sure in five or ten years they'l l get more sense into it. They should really consider using different reference groups instead of berry, skunk or what have you. Now it's like determining how much neanderthals had genes from modern New Yorkers.
You hit the nail on the head. That was what I tried to explain before.

About 2% genes from modern New Yorkers can be found in Neanderthals as Phylos Galaxy establish with his Skunk, Berry, OG Kush,... groups. It could be the proof time travelling will be possible

You need a well choosen outgroup to understand a cladogram. Or you will have chickens are the ancestors of Tyrannosaurus.

Quote:
An outgroup that is nested within the ingroup will, when used to root the phylogeny, result in incorrect conclusions about phylogenetic relationships and trait evolution.
https://en.wikipedia.org/wiki/Outgro...ce_of_outgroup

I have seen some seedbanks sell seeds under the same name, and the ones from a few years ago looked like pure NLD and today they resemble more a highly BLD.

I wonder what seeds they sent to Phylos.
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#78
Old 03-31-2018, 09:14 PM
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Last edited by Water-; 04-01-2018 at 01:54 AM.. Reason: he had it right
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#79
Old 03-31-2018, 09:39 PM
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Quote:
Originally Posted by herbgreen View Post
PANAMA ACE:

2 generations of Panama 74 x Panama Red Hair
1 generation of Panama 74 / Red Hair F2 x 3 males Panama Network of third line different
4 generations of this triad plus and voila (current seeds of Panama ACE for sale in regular and feminized)

https://www.cannabiogen.com/club/vie...=1653&start=30

Some descriptions include punto rojo but the ACE site has down as 3 panama strains

Anyone know the differences panama goddess and A22? thanks!


Ace version is very worked and developed and uses 1974 panama red standard regular seeds and fem

Greenthumb seems more wild and only feminzed
I love that kind of post, missing Charlie....
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#80
Old 04-03-2018, 12:50 AM
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panama red 1974

https://www.icmag.com/ic/showthread.php?t=196211
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