Tissue Culture : Micropropagation - Page 2 - Cannabis Business Network - International Cannagraphic Magazine Forums

ambertrichome · 03-07-2018, 02:57 AM

I had what I had. This was in 1995.

It worked though.

Vapedoutdude · 03-07-2018, 03:31 AM

Phinestcannabis is offering tissue culture clone for sale in sac town I believe

led05 · 03-07-2018, 02:18 PM

Quote:

Originally Posted by ambertrichome

I had what I had. This was in 1995.

It worked though.

I think the Tech came along in the 50 or 60's but not exactly widely known without the Net like these days...

Either way, I bet your friends and family, shit even me, would have paid to see you in your get-up, all saran wrapped up, no eyebrows, clothes in the lab......

very cool you were doing that back then

ambertrichome · 03-07-2018, 03:09 PM

I'm pretty sure this is how the Dutch do their Tulips ect, and why they are so uniform. I believe they've been dong this for decades.

There is also No Genetic Drift in TC vs clones.
TC takes the plant back to the embryonic stage. At least that was the info way back then. I don't claim to know a lot technically about it, just that its been done a long time, and kits have been available since at least the 90s.

beta · 03-07-2018, 03:29 PM

Quote:

Originally Posted by ambertrichome

There is also No Genetic Drift in TC vs clones.
TC takes the plant back to the embryonic stage. At least that was the info way back then. I don't claim to know a lot technically about it, just that its been done a long time, and kits have been available since at least the 90s.

There is no 'genetic drift' in clones, either. 'Genetic drift' is entirely made up.

**Sam_Skunkman** · 03-07-2018, 03:34 PM

I agree there is no drift, there are mutations that seldom happen, and clones can pick up viruses that can effect performance like yield and vigor.
-SamS

marmarb · 03-07-2018, 03:40 PM

@Sam_Skunkman any kits on the market that you would vouch for.

Gonna use a empty 5x5 grow tent as my base of operation.

**Sam_Skunkman** · 03-07-2018, 04:32 PM

No suggestions, I built a TC lab in an extra room in my lab several decades ago that I used for Cannabis, it was not as hard as I thought it would be, I did have all the infrastructure like a Laminar Flow Hood to work under, and a refrigerator with grow TL's to keep my plants in tubes alive and veg.
My goal was simple, I wanted to be able to freeze meristems, callus and be able to thaw it out later and regrow. My goal was to have a library of 1000 plants in a small freezer as a back up, in several different countries. I was unsuccessful, a bit before it could be done, today it can be done.
When I did it the meristems after freezing turned into black slime, no luck with callus either, but others have done it today.
I predict soon like grape vines for wine, and strawberries, all Cannabis plants for growers will be produced by TC by companies like SEGRA and guaranteed to be virus free and vigorous clones.
Sounds good to me.
-SamS

led05 · 03-07-2018, 07:52 PM

Quote:

Originally Posted by Sam_Skunkman

all Cannabis plants for growers will be produced by TC by companies like SEGRA and guaranteed to be virus free and vigorous clones.
Sounds good to me.
-SamS

gosh I hope not, not ALL at least.... Seeds and the unknown have their places

**Sam_Skunkman** · 03-08-2018, 12:25 AM

Quote:

Originally Posted by led05

gosh I hope not, not ALL at least.... Seeds and the unknown have their places

And in the wine industry why do you think all growers use virus free certified grape vines? No one grows wine from seed plants. Same with strawberries and other crops.
I agree that seeds are handy for breeding and finding new elite clones to mass produce. But in the future most Cannabis in the West may well be TC.
-SamS

03-07-2018, 02:57 AM	#11
ambertrichome Member Join Date: Mar 2013 Posts: 143	I had what I had. This was in 1995. It worked though.
	Quote 1 members found this post helpful.

03-07-2018, 03:34 PM	#16
Sam_Skunkman "RESIN BREEDER" Join Date: Mar 2004 Posts: 4,657	I agree there is no drift, there are mutations that seldom happen, and clones can pick up viruses that can effect performance like yield and vigor. -SamS
	Quote 2 members found this post helpful.

03-07-2018, 03:40 PM	#17
marmarb Member Join Date: Apr 2010 Location: prop deez Posts: 495	@Sam_Skunkman any kits on the market that you would vouch for. Gonna use a empty 5x5 grow tent as my base of operation. __________________ Going Vert no Dirt
	Quote

03-07-2018, 04:32 PM	#18
Sam_Skunkman "RESIN BREEDER" Join Date: Mar 2004 Posts: 4,657	No suggestions, I built a TC lab in an extra room in my lab several decades ago that I used for Cannabis, it was not as hard as I thought it would be, I did have all the infrastructure like a Laminar Flow Hood to work under, and a refrigerator with grow TL's to keep my plants in tubes alive and veg. My goal was simple, I wanted to be able to freeze meristems, callus and be able to thaw it out later and regrow. My goal was to have a library of 1000 plants in a small freezer as a back up, in several different countries. I was unsuccessful, a bit before it could be done, today it can be done. When I did it the meristems after freezing turned into black slime, no luck with callus either, but others have done it today. I predict soon like grape vines for wine, and strawberries, all Cannabis plants for growers will be produced by TC by companies like SEGRA and guaranteed to be virus free and vigorous clones. Sounds good to me. -SamS
	Quote 1 members found this post helpful.

03-07-2018, 03:31 AM	#12
Vapedoutdude Newbie Join Date: Oct 2015 Posts: 16	Phinestcannabis is offering tissue culture clone for sale in sac town I believe
	Quote

03-07-2018, 03:09 PM	#14
ambertrichome Member Join Date: Mar 2013 Posts: 143	I'm pretty sure this is how the Dutch do their Tulips ect, and why they are so uniform. I believe they've been dong this for decades. There is also No Genetic Drift in TC vs clones. TC takes the plant back to the embryonic stage. At least that was the info way back then. I don't claim to know a lot technically about it, just that its been done a long time, and kits have been available since at least the 90s.
	Quote