[EugeneOregon]

Originally Posted by G.O. Joe

Chromatographic and Spectroscopic Data of Cannabinoids from Cannabis sativa L. by Arno Hazekamp, Anja Peltenburg, and Rob Verpoorte.



No one has to do any analyses to see that you don't know the difference between m/z from a MS and nm from a spectrophotometer, and don't care to know any time soon. The charts I posted show little THC absorbance at 299 nm certainly not a peak, and I just plain dispute everything you have to say, but don't care much. If you are saying you have something with a maxima at 299, this is proof that it isn't pure THC. Maybe it has THC in it, but whatever is in it has a maxima at 299. This is assuming there is nothing wrong with your solvent, machine, and cuvette. The last chart shows the difficulty in quantifying THC by UV in the presence of traces of CBN.

I wish my spectrophotometer measured UV but it's a much cheaper visible only model, useful for determination of the particular chromophore formed from a cannabinoid and Fast Blue, once calibration curves from analytical standards have been determined of course. My LC-MS is short one $1000 DAD flow cell, and nitrogen generator. Bong hit for 420.

Perhaps you can borrow somebody elses stuff and post corrected data with a machine that has the same capabilities as mine? After all, the original post was about my instrument producing a spectral scan. Why don't you make a chart for yourself and apply your own wisdom to the problem? Confirm your own hypothesis by direct observation of your own work, which is infinitately more difficult than flaming a post about somebody else's, right? I am sorry if I bruised your ego. My intent was to share knowledge, and not to piss you off personally lolz. Grow up.

Good luck sport.

[G.O. Joe]

This is why I've avoided responding to your posts - this could be the only outcome as if by script. All I did was put it in the most delicate way, yet this was still unavoidable.

[EugeneOregon]

I can assure you sir I will not post on icmag again. They have all they need with you.

[SkyHighLer]

TLC to determine relative cannabinoid levels was superbly explored by Sadpanda with the assistance of G.O. Joe last year.

Sadpanda comes in at post #35 (page 4.)

https://www.icmag.com/ic/showthread.php?t=148375

[G.O. Joe]

I don't deserve any undeserved credit at all. That homework and a plan went well, all respect goes to sadpanda. TLC with maybe the anisaldehyde-sulfuric acid visualization reagent mentioned there could have come in handy here to indicate purity, if that was a goal. Gene O. might post here one last time - you'll have to log in to a different site to view it - he'll be plotting something like that last graph? Maybe that's what he did in this one, but he didn't say much here. Absorbance pickup at 299-300 certainly does maybe indicate THC - when it's just the lowest amount definite beginning/end of it.