[phdilly]

Great thread, it makes sense to want to sequence in house and when the upfront cost of one of those units right around 1K it makes you think you should get one and use it for marker assisted selection in your breeding experiments. The quick answer is yes, ONT nanopore sequencing works.

The major misconception is that it is not sufficient to blindly sequence the genomes of your cannabis plants. Sequencing a genome is the easy part, especially with a mobile devise that plugs into your laptop.

I am sure we all know that in order to breed, you must be selecting for a particular trait of interest, otherwise what's the point? Well same applies to the genomic data that comes out of these little sequencers. One must first identify and select the regions of the genome associated with the trait of interest, characterize the variation in your population and then develop diagnostic tools that will aid in your efforts to identify the emergence of the trait earlier in development so you can streamline the process.

That's the hard part. My current opinion, Minion is not the answer to marker assisted selection, unless you are able to capture a targeted region of the genome which is associated with your traits of interest and you can use it to provide diagnostic markers for your traits.

Peace