GA3: How to germinate old seeds? - Page 5 - Breeder's Laboratory - International Cannagraphic Magazine Forums

Beuh-chat · 09-26-2017, 12:05 PM

Hi
I have to try with my 50 years old seed from congo

HaploidDoubler · 03-02-2018, 12:41 AM

Gibrellic acid is a hormone (an inducer) that induces germination and sprouting in seed and plant tissue calluses. It may induce phenotypic irregularities, but it does not induce genetic mutation that I’m aware of. Think of it as telling the genome what “program” to run, like a batch file or something for you programmer nerds out there! Anyway, the stuff has its uses!
I use a media impregnated with Gibrellic acid at work in order to get sprouts to form from a callus. It’s also of course used for germination. In my agar based media we use 500ml Deionized water, MS (forgot exact amount, I may update this post later if it’s useful to anyone), 30 grams sucrose (sugar), pH balance to 5.8 to optimize nutrient uptake, we bake it in an autoclave, you could use a pressure cooker, and after it cools to a temp cool enough to handle (90*F or so) we add 500ul GA3, too hot and it will denature! This media is for double haploid calluses to produce shoots we can clone. Maybe it could be useful, maybe not. Growing in this stuff makes them neutrotrophic, they just suck up sugar and media and don’t rely on photosynth, you gotta “harden” them before potting in soil or whatnot. It’s a lot like plant life support. I work currently on brassica at a University, I’d like to ply my skills to Cannabis breeding in the industry when I graduate. This seems like a great place to learn the trade, I’m just trying to give back by sharing where I can! Hope this tidbit was useful to someone!

Sport Farmer · 03-02-2018, 10:54 PM

I've been trying to germ a bunch of old seeds (20+ years) for awhile now and I haven't been successful at all. I've had a few crack and then just give up... but thats about it. I bought some GA3 about a year or so ago, sandpapered them down a little and let them soak in that for 24-36 hours and that didn't even do the trick for me... and they give you like the most meager of amounts of that GA3 when you order it... Just enough to piss you off.

I'm gonna try and mix up some kelp solutions and try the clonex suggestion too as I have both of those in my arsenal... At this point I'm open to anything because all the usual suspects aren't working for me... and I've tried!!!

09-26-2017, 12:05 PM	#41
Beuh-chat Member Join Date: Jun 2017 Posts: 50	Hi I have to try with my 50 years old seed from congo
	Quote

03-02-2018, 12:41 AM	#42
HaploidDoubler Newbie Join Date: Mar 2018 Posts: 2	Gibrellic acid is a hormone (an inducer) that induces germination and sprouting in seed and plant tissue calluses. It may induce phenotypic irregularities, but it does not induce genetic mutation that I’m aware of. Think of it as telling the genome what “program” to run, like a batch file or something for you programmer nerds out there! Anyway, the stuff has its uses! I use a media impregnated with Gibrellic acid at work in order to get sprouts to form from a callus. It’s also of course used for germination. In my agar based media we use 500ml Deionized water, MS (forgot exact amount, I may update this post later if it’s useful to anyone), 30 grams sucrose (sugar), pH balance to 5.8 to optimize nutrient uptake, we bake it in an autoclave, you could use a pressure cooker, and after it cools to a temp cool enough to handle (90*F or so) we add 500ul GA3, too hot and it will denature! This media is for double haploid calluses to produce shoots we can clone. Maybe it could be useful, maybe not. Growing in this stuff makes them neutrotrophic, they just suck up sugar and media and don’t rely on photosynth, you gotta “harden” them before potting in soil or whatnot. It’s a lot like plant life support. I work currently on brassica at a University, I’d like to ply my skills to Cannabis breeding in the industry when I graduate. This seems like a great place to learn the trade, I’m just trying to give back by sharing where I can! Hope this tidbit was useful to someone!
	Quote 1 members found this post helpful.

03-02-2018, 10:54 PM	#43
Sport Farmer Member Join Date: Feb 2006 Location: Midwest Posts: 298	I've been trying to germ a bunch of old seeds (20+ years) for awhile now and I haven't been successful at all. I've had a few crack and then just give up... but thats about it. I bought some GA3 about a year or so ago, sandpapered them down a little and let them soak in that for 24-36 hours and that didn't even do the trick for me... and they give you like the most meager of amounts of that GA3 when you order it... Just enough to piss you off. I'm gonna try and mix up some kelp solutions and try the clonex suggestion too as I have both of those in my arsenal... At this point I'm open to anything because all the usual suspects aren't working for me... and I've tried!!!
	Quote