[BombBudPuffa]

This is an awesome thread! Darpa do you mind if I experiment alongside you? I wonder if a side graft would work.

[heady blunts]

cool projects darpa. i'll definitely be keeping an eye out for your threads.

the project i always had in mind for canna grafting was to graft slow vegging/no stretch wld heirloom scions to vigorous f1 rootstocks in hopes of improving growth rate.

i've often wondered about using a hop root stock with a canna shoot since those vines are so massive and low maintenance, and closely related.

[Darpa]

Originally Posted by SuperWeed

Tell me you're in Oregon. I need more friends like you Darpa.

Hi SuperWeed, unfortunately I’m about 3000 miles from Oregon…

Originally Posted by BombBudPuffa

This is an awesome thread! Darpa do you mind if I experiment alongside you? I wonder if a side graft would work.

You are more than welcome to jump along! I would love to see other people success with different grafting technique. I did performe some auxiliary bud side graft (chip graft) if you check my previous post (experiment No2) This is quite similar to the veneer side graft technique, but I prefer to work with small plant material for the scion. I just removed the wrapping today and everything fused together just fine.







Here is the result of experiment 3 (insertion of a scion in an vertical incision performed in the internode section of the stem)

Fresh graft:


One week old graft, unwrapped and perfectly fused.

Originally Posted by heady blunts

cool projects darpa. i'll definitely be keeping an eye out for your threads.



the project i always had in mind for canna grafting was to graft slow vegging/no stretch wld heirloom scions to vigorous f1 rootstocks in hopes of improving growth rate.

i've often wondered about using a hop root stock with a canna shoot since those vines are so massive and low maintenance, and closely related.

I would like to try a similar experiment but I would graft my Biker Kush, which is a phenomenal potent strain with an extremely slow root system development, on the rootstock of my vigorous Sour Diesel strain just to see If the BK would develop faster. I have alse a pure sativa landrace from Swaziland that I would like to use as a rootstock on my current cross (BKGW) in order to document the possible phenotype alteration compared to the same un-grafted BKGW plant (side by side comparaison). The effects on the phenotype of a grafted cannabis plant associated with the transport of RNA from the rootstock to the scion are poorly documented for cannabis.

[Darpa]

Good evening everybody! Here we go with experiment No 5: The Potato - Cannabis graft...

I selected few old potato that were going right in the trash but I decided to cover them with soil for 5 to 6 day...



Here is what I got. I should have wait few more days until the stems develop a little bit more.



Then I selected two apical scions from a mother plant which I trimmed and cut the stem in a V-shape wedge with a surgical blade:



I inserted the scion wedge into the cleft:

[Darpa]

I did a similar operation on the other stem of the potato and I wrapped everything tightly with plastic wrap.



Here is the final result. I will rest in the dome for at least 5 to 7 days. I'll keep you updated!



As I said before, we are no where close to a taxonomic proximity which is a general prerequisite for successful graft-take and long-term survival of the grafted. I should give it try on roses plant which would probably work...

[Betterhaff]

It’s been generally recognized that the success of grafting declines as you move up taxonomic rank (although not necessarily absolute). The success of Species to Species > to Gender > to Family > to Order, etc.

Remember the Simpsons episode where Homer creates the tomacco plant. It’s been done in real life and there were traces of nicotine found in the grafted tomato plants leaves. I guess the question is does nicotine naturally occur in tomato plants. Tobacco and tomatoes are in the same family.

[Darpa]

Originally Posted by Betterhaff

It’s been generally recognized that the success of grafting declines as you move up taxonomic rank (although not necessarily absolute). The success of Species to Species > to Gender > to Family > to Order, etc.

Remember the Simpsons episode where Homer creates the tomacco plant. It’s been done in real life and there were traces of nicotine found in the grafted tomato plants leaves. I guess the question is does nicotine naturally occur in tomato plants. Tobacco and tomatoes are in the same family.

Tomato fruits and leaves naturally contain a low level of nicotine (2.4–6.0µg kg−1). Tomato fruits and leaves naturally contain a low level of nicotine (2.4–6.0µg kg−1). However when you graft a tomato scion on a tobacco rootstock, the level of nicotine found in the fruit is significantly increased. We have to remember that nicotine is synthesized in the root of the Tobacco plant and then transported to the leaves and the lateral parts of the plant, and its quantity depends on the type of tobacco, nitrogen availability, temperature, light, moisture and injury. Fruits of tobacco-grafted tomato cultivars had been found to contain almost a hundred times higher amounts of nicotine than those of selfrooted plant. Interesting fact, beside the increase of Nicotine, research have demonstrate that Tomato-Tobacco grafting resulted to a 30% increase of total fruit weight compare to ungrafted tomato plant!




Grafting tomato plant on tobacco plant and its effect on tomato plant yield and nicotine content, First published: 17 March 2009

Abstract
BACKGROUND: Two different tomato scions, cv. Elazig and cv. Sweet (cherry) (Solanum lycopersicum L.) were self-grafted and grafted onto tobacco root stock (Nicotiana tobacum L.). Then, grafted tomato plants were evaluated in terms of flower and fruit yield. Tobacco-grafted tomato plant fruits were also evaluated for nicotine content.

RESULTS: Tobacco grafting had a positive effect on the tomato plant cultivation performance; the onset of flowering was almost 15 days earlier and the tomato flower and fruit yields increased in both tomato cultivars. Tobacco grafting resulted in 5.0% and 30.1% increase in total fruit weight for cv. Sweet and cv. Elazig, respectively. Because the level of nicotine was within acceptable ranges, tobacco-grafted tomato fruits were considered to be safe for consumption. Self-grafted tomato cultivars also had flowering time onsets almost 11 days earlier. However, self-grafting caused 6.0% and 7.6% less total fruit yield per cv. Sweet and cv. Elazig, respectively.

CONCLUSION: In conclusion, our results show that tomato–tobacco grafting is a novel and promising technique for improvement of not only tomato plant performance and yield, but also that it can be employed to various tomato varieties.

[SuperWeed]

Originally Posted by Darpa

Tomato fruits and leaves naturally contain a low level of nicotine (2.4–6.0µg kg−1). Tomato fruits and leaves naturally contain a low level of nicotine (2.4–6.0µg kg−1). However when you graft a tomato scion on a tobacco rootstock, the level of nicotine found in the fruit is significantly increased. We have to remember that nicotine is synthesized in the root of the Tobacco plant and then transported to the leaves and the lateral parts of the plant, and its quantity depends on the type of tobacco, nitrogen availability, temperature, light, moisture and injury. Fruits of tobacco-grafted tomato cultivars had been found to contain almost a hundred times higher amounts of nicotine than those of selfrooted plant. Interesting fact, beside the increase of Nicotine, research have demonstrate that Tomato-Tobacco grafting resulted to a 30% increase of total fruit weight compare to ungrafted tomato plant!




Grafting tomato plant on tobacco plant and its effect on tomato plant yield and nicotine content, First published: 17 March 2009

Abstract
BACKGROUND: Two different tomato scions, cv. Elazig and cv. Sweet (cherry) (Solanum lycopersicum L.) were self-grafted and grafted onto tobacco root stock (Nicotiana tobacum L.). Then, grafted tomato plants were evaluated in terms of flower and fruit yield. Tobacco-grafted tomato plant fruits were also evaluated for nicotine content.

RESULTS: Tobacco grafting had a positive effect on the tomato plant cultivation performance; the onset of flowering was almost 15 days earlier and the tomato flower and fruit yields increased in both tomato cultivars. Tobacco grafting resulted in 5.0% and 30.1% increase in total fruit weight for cv. Sweet and cv. Elazig, respectively. Because the level of nicotine was within acceptable ranges, tobacco-grafted tomato fruits were considered to be safe for consumption. Self-grafted tomato cultivars also had flowering time onsets almost 11 days earlier. However, self-grafting caused 6.0% and 7.6% less total fruit yield per cv. Sweet and cv. Elazig, respectively.

CONCLUSION: In conclusion, our results show that tomato–tobacco grafting is a novel and promising technique for improvement of not only tomato plant performance and yield, but also that it can be employed to various tomato varieties.

I must spread some reputation around before giving it to Darpa again.

[Darpa]

Originally Posted by BombBudPuffa

This is an awesome thread! Darpa do you mind if I experiment alongside you? I wonder if a side graft would work.

This one is for you BombBudPuffa!

Grafting experiment No 6: 2 Veneer Side Graft on one plant.

[Darpa]

I realized that the picture are pretty small... so here is the veneer side graft before wrapping.