[Darpa]

And finally everything is firmly wrapped

[SuperWeed]

Originally Posted by Darpa

Thank SuperWeed! I wish I kept all the genetic I've work with in the past. I had some of the best genetic from the Super Sativa Seeds Club at the end of the 90th and I wish I had save them... What happened with your SuperMom in 2015?

Cops.

[Darpa]

7 days is all you need to make a viable and healthy graft. Here an update on the grafting experiment. All 9 graft are doing fine and their auxiliary buds doubled in size within just a couple of days.



Here is an example of the fusion between the scion and the rootstock steam (7 day after the initial V-Graft)



Closer look:

[2000pm]

Cool stuff, how are you wrapping these? is that just saran/plastic wrap?

[Darpa]

Originally Posted by 2000pm

Cool stuff, how are you wrapping these? is that just saran/plastic wrap?

There is grafting tape or grafting clip that can be use, but I personally use saran / plastic wrap that work just fine... just cheaper and you get the same result.

[Darpa]

Ok, here we go again with an other experiment, since all of the V-Graft (wedge graft) and the auxiliary buds side grafting (chip grafting) worked with a 100 % success rate, here is a trial of a trunk and root system fusion.

Here is the two female specimen used in the experiment:



So I cut half of the root system on the two plant (in order to make full contact below and above ground):



I made an vertical incision on each stem of about one inch in order to expose the cambium. I should have use a bigger container but the were inserted really closes one to the other in the same pot:



Then, as usual, they were firmly wrapped together:

[Darpa]

Here is the final result:



It will rest under the dome for at least 5 day...



Next experiment: Grafting on a potato stem. Why? Why not!

[SuperWeed]

Originally Posted by Darpa

Here is the final result:

View Image

It will rest under the dome for at least 5 day...

View Image

Next experiment: Grafting on a potato stem. Why? Why not!

View Image

Too far removed genetically?

Mad respect Darpa

[Darpa]

Originally Posted by SuperWeed

Too far removed genetically?

Mad respect Darpa

Thank SuperWeed,
As you can see in post 96, the potato graft experiment is intended to demonstrate that the taxonomic proximity is a general prerequisite for successful graft-take and long-term survival of the grafted, composite plant. This will illustrate, in a real experiment, the effect of the phylogenetic distance between the potato plant and the cannabis plant on the graft success. (I’ve saw people saying that it was possible on some post) I already know that the result is most likely to be negative, but this is just a way to show it to the community. Nothing is better than pictures!

If you would like me to experiment any other type of grafting technic, let me know! After this grafting demonstration, I will probably be posting step by step pictures for basic tissue culture, callus induction, and if I get my personal lab ready soon enough, protoplasm fusion, cell transformation with agrobacterium bacteria and artificial production of doubled haploids in order to get complete homozygosity in only one generation compared to traditional breeding technique. I’m a curious biochemist that really like to experiment! I have so many project in mind, but it’s mostly to show people what you can do when you dig a little bit deeper. It's all about science!

[SuperWeed]

Originally Posted by Darpa

Thank SuperWeed,
As you can see in post 96, the potato graft experiment is intended to demonstrate that the taxonomic proximity is a general prerequisite for successful graft-take and long-term survival of the grafted, composite plant. This will illustrate, in a real experiment, the effect of the phylogenetic distance between the potato plant and the cannabis plant on the graft success. (I’ve saw people saying that it was possible on some post) I already know that the result is most likely to be negative, but this is just a way to show it to the community. Nothing is better than pictures!

If you would like me to experiment any other type of grafting technic, let me know! After this grafting demonstration, I will probably be posting step by step pictures for basic tissue culture, callus induction, and if I get my personal lab ready soon enough, protoplasm fusion, cell transformation with agrobacterium bacteria and artificial production of doubled haploids in order to get complete homozygosity in only one generation compared to traditional breeding technique. I’m a curious biochemist that really like to experiment! I have so many project in mind, but it’s mostly to show people what you can do when you dig a little bit deeper. It's all about science!

Tell me you're in Oregon. I need more friends like you Darpa.