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| Forums > Marijuana Growing > Marijuana Strains and Breeding > Breeder's Laboratory > simple, detailed, hermetic pollen collection and pollen release in vials. 46 | ||
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simple, detailed, hermetic pollen collection and pollen release in vials. 46
simple, detailed, hermetic pollen collection
i, and probably many others, need simple, detailed instructions on hermetic pollen collection. i have read it is possible to cut the sacks and have them develop off the plant. i request detailed procedure on clipping sacks and how to store them, so that they will finish development and release pollen in a sealed container. ~will they ripen/release in a ziplock bag or sealed 1-2ml vial? ~do they need air, like say a micropore tape or breathable membrane like tyvek over the cap? ````````````` ~do they need light? ~when should they be harvested so it is not to early and not to late that other sacks on the plant release and contaminate the females? for the purpose of, serious, micro breeding, it has come to my realization that, if many strains, say 23, are to be bred in any numbers, and those are also long flowering sativas, it becomes an endeavour that would take years, to re-produce each one singly by open pollination of a mono-strain (to preserve the stock gene diversity), let alone make crosses, even one time crosses. making more boxes can help but having the ability to grow multiple strains, hermetically collect the male pollen, cull the males (really unfortunate*) and use one male strain per mixed female batch would ensure keeping track of genetic/parental origin and give multiple crosses in one crop *maybe a male father plant clone chamber can be kept in vegetative and the labeled male (and female) plants can be saved; but, this takes up alot of space, for even one batch, for a micro grower/breeder, though and is not the focus of these questions/unknowns. estimating: if a crop was 120days (12/13 or 11/14 light cycle) to flower and was 3-5weeks to end vegetative growth under 12/13 or 11/14 light cycle (21-35days) from seed, then 141 or 155days would be 2 harvests of pure sativa a year. so at most, two strains could be preserved/reproduced and at least one cross could be made (more plant strains could be planted but only 2 times would using single male strain pollinators be possible). this is still stressful to someone doing work. not having knowledge of collecting male sacks makes it much more so. the biggest issue seems to be strain integrity/lineage so if a person only has one box/area and grows 3 strains, the sacks can be harvested before they contaminate everything. then each of the 3 strains could be pollinated by one strain of males per crop. then 2 more repeats of the same thing can be done in the next 2 crops, using then the 2nd and then 3rd male strains to pollinate. then your mobile/untrackable vectors, the males, are single and a scientific control (if that is the term). the females if labeled are stationary and so the seeds can be saved knowing there was a strain reproduction XX ,and a YXcross and a ZXcross. each succesive breed (1,2,3) would give more varied male stock as the pollen sacks were collected each time. batch number 2 would have 1,2 male plant batch pollen and batch number 3 would have 1,2,3 male plant batch pollen. so 3 runs would give XX, YY, ZZ reproductions, and XY, XZ, XZ(here it is generalized with no specific male female order). to be more precise, if F/M X1/X1____Y1/X1____Z1/X1 X2/Y1,2__Y2/Y1,2__Z2/Y1,2 X3/Z1,2,3_Y3/Z1,2,3_Z3/Z1,2,3 where the numbers are the batches; also assuming female clones werent taken and new ones from new batches were used, otherwise... X1/X1____Y1/X1____Z1/X1 X1,2/Y1,2__Y1,2/Y1,2__Z1,2/Y1,2 X1,2,3/Z1,2,3_Y1,2,3/Z1,2,3_Z1,2,3/Z1,2,3 this would also give male to female and female to male variants in crosses, where the generalized XX XY XZ form would lack info. there are botanical/scientific names for this that i don't know. this illustrates the breakdown better for making this point though. while it may be a knee jerk reaction for regular (whatever that means) breeders to have no serious thoughts about micro breeding, it is nonetheless seriously important. many people are surrounded by cicumstances that make micro growing and stealth the only option. think of how many people live in population centers and outside sativa friendly latitudes. those people could each and all breed pure sativas (or any other types) and keep them from going extinct, all the while, also evolving them. the people who get the original landraces can continue releasing them and there would also be a larger group of breeders, testers and preservers, who also might be able to network better (safeguards for strain lines?) with the help of ic mag, "macgyver"ing it, and science, i believe it is possible to do, with will/focus. it would also be interesting to see, in decades of unconventional breeding, if sativas kept in yogurt cups (instead of selected for shortness of height) would still be monsters if used for growing or re-establishing gene lines outdoors in the future (when people are maybe less crappy). i'm hoping this furthers science and hacking/making/DIY so people can find the wonder of (edit: assisting/re?) creation for themselves, no matter where they live or whatever turdheads write/smear turd on paper. now i think is the time to do it, while we can. every person who can get the data can carry a torch from it. simply, this would destroy commercial patenting if it becomes legal in countries like the us and would prevent the perversion of the plant. the best would be in the hands of the people. it is terrible to imagine monopolies on a sacred plant. with it having been criminalized by perverse people, only to have it grabbed up and controlled under new "law" by people with enough power/money to legalize it and form big companies in the first place. so before any shit akin to that happens, i suggest people learn to also master the tiny, so it is safe from say, gmo pollen in the future and all the untold nastiness that could occur. already, today, there are farmers having problems with their crops being pollinated by monsanto pollen and they, not monsanto facing lawsuits about it. so please think about how marijuana currently has a grace period and that hindsight is 20/20. Last edited by earthwyrms; 12-04-2015 at 12:20 AM.. |
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#2 |
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Location: NY
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pollen capture/storage is the same for hermie nanners or 'regular' nanners
trick is to dry it and then freeze it, at least for long term storage my own way is to pluck a ripe banana cluster with tweezers, then tap it to release pollen on to a drying surface i use porous paper, then goes into a container of dry rice which will dry it further after 24 hours into the fridge, then 24 hours later the freezer but there are many other ways to achieve this end
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#3 | |
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Quote:
"Hermetic" (sealed airtight), not hermaphroditic.
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nature is wise. always keep it real. |
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#4 |
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i noticed that after i posted, i see herm and thoughts of bananas dance through my head
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