[live resin]

1. Extract fresh plant material with 0.1% acetic acid v/v in hexane. Filter and remove solvent by rotavap. The purpose of the acidified extraction solvent is to minimize ionization of the cannabinoid acid which would hinder subsequent purification steps

2. Pass the THCA rich solution through a column packed with Sephadex-LH20, eluting with 2:1 chloroform/dichloromethane. Collect the THCA-rich fraction.

3. Redissolve the crude THCA in methanol, filter insoluble material, remove solvent with rotavap

4. Redissolve crude THCA in pentane, filter, and rotavap to give THCA crystals

* All solvents and chromatography materials were obtained off the shelf from a local lab supply shop. 10L rotavap obtained from BHOgart

[live resin]

I know I'm not the first to see this patent for this and copy the procedure, has anyone else had success with this?

[G.O. Joe]

Originally Posted by live resin

* All solvents and chromatography materials were obtained off the shelf from a local lab supply shop. 10L rotavap obtained from BHOgart

Originally Posted by live resin

I know I'm not the first to see this patent for this and copy the procedure, has anyone else had success with this?

What was your column loading ratio? What was the flow rate and how many column volumes of solvent were necessary to start and complete the elution of THCA, and how long did that take? How much did the Sephadex swell with that solvent and how much did it take to fill the column and how much did that cost? How is the Sephadex cleaned for reuse?

The patent, I do know is US 7,700,368. Those details are not in it.

[thegreenman91]

What kinda yield using this method?

How many hours of work total?

[G.O. Joe]

Is someone getting quotes on the sort of shiny new industrial gel chromatography system you guys would want? We won't tell.

The patent specified a column 1.5 meters tall filled to an unspecified height with 400 g. of the Sephadex, loaded with the dissolved extract of 100 grams of something. Let's say it was 20 grams of extract - 1:20 column loading is not bad at all. But, the LH-20 product instructions suggest a flow rate of 1-10 cm./hr. Compare this to the level of detail in El-Sohly's 6,730,519.

There are faster, dirtier, much, much cheaper methods that scale up well.
https://www.erowid.org/archive/rhodi...atography.html

[oceangrownkush]

Would crystalline THC be snortable? I would love to rack up lines of Hash.

[Phineas Freak]

I guess THC can be taken up somehow by mucous membranes, analog to Sativex.

I wonder what the effect of eaten THCA is?
Has anybody tried? Is it psychoactive or not? Does it affect pain?

[Avinash.miles]

[live resin]

12k for the factory refurbished chromatography system. The 10L rotavap was 8k

[oceangrownkush]

Couldn't you just purge it like BHO? I know people use Hexane to make extracts and don't spend 12k on their desiccation chamber. Has to be an alternative to the rotovap.