[Skinny Leaf]

Originally Posted by Sam_Skunkman

You can get most science papers for free at:

sci-hub.io

Tried there first. Did not see it. Of course I could have overlooked it. I used the doi number to search for it, but, it kept sending me to the place I finally bought the paper from. Probably had to pay for it since the research was done at the U.S. government's cannabis growing facility.

[Sam_Skunkman]

I just cut and pasted the title:
In vitro mass propagation of Cannabis sativa L.: A protocol refinement using novel aromatic cytokinin meta-topolin and the assessment of eco-physiological, biochemical and genetic fidelity of micropropagated plants
Into the sci-hub.io site.
And it popped up immediately.
You can use doi, or other search methods also.
Sometimes the site is down for a few hours.
-SamS

[Sam_Skunkman]

https://cms.herbalgram.org/heg/volume...ca01f36c827756

HerbalEGram: Volume 13, Issue 4, April 2016

Cannabis Taxonomy: The 'Sativa' versus 'Indica' Debate

By Robert C. Clarke and Mark D. Merlin, PhD


I agree except I still use the WLD instead of BLD, Karl Hillig first used WLD. Regardless we do agree, as a BLD or WLD both refer to a Wide or Broad Leaf Drug variety.
I think Rob likes to avoid folks thinking WLD is for wild so he uses BLD. I have also seen BLDB for Broad Leaf Drug Biotype, I don't bother with Biotype.
As long as WLD and NLD varieties are considered Indica, and NLH and WLH is considered Sativa I agree. Although in China there are WLD or WLH varieties that are not really low drug, (THC) they can be used for hemp or seeds and are a bit druggy, or select ones are.

-SamS

[Sam_Skunkman]

https://www.medicinalgenomics.com/cannmed2016/

You can download each of the presentations, check out at least: RYAN C. LYNCH
MEDICINAL GENOMICS, KEVIN MCKERNAN COURTAGEN LIFE SCIENCES, MARK A. LEWIS
PRESIDENT OF NAPRO RESEARCH, ETHAN RUSSO MEDICAL DIRECTOR PHYTECS, JOSH WURZER SC LABORATORIES, RAPHAEL MECHOULAM KEYNOTE SPEAKER

The future of Cannabis will be DNA marker assisted breeding, these guys are preparing the way, you do not have to use GMO to see the gains their insights will bring to classical breeders like me. The work is amazing, I love the NAPRO approach to Cannabinoid and terpene analysis, it is how to do it today.
-SamS

[Sativied]

I just read the presentation by Mark A. Lewis ( https://www.medicinalgenomics.com/wp-...sentation.pptx ), very good and readable for everyone, thanks for sharing.

"Indica and sativa may be better suited as an ‘effects gauge’ instead of genealogical nomenclature"
"-Now there is “mostly sativa”, “mostly indica”, “sativa-like, indica-blah”, etc.
-Says little to the consumer about the product they are purchasing"

"Blah" puts it nicely...

The WLD/NLD you mentioned in the other post above is imo mostly interesting for pure varieties. I often see people label NLD as sativa-dom, suggesting the leaflet/leaf shape is linked to every other 'sativa' trait. They kind of do the same thing in the napro research, suggesting leaf shape and chemotype genes are linked. I don't think that is true in all polyhybrids... and I don't think that will hold true for future modern bred cultivars. The leaf shape of the latter will depend on the desired structure for farmers. As much as I like narrow leaflets (open structure, more branching / weaker apical dominance) if there's no plant count limit then growing single cola wide leaf plants may be desirable. It just seems wrong to group cultivars based on a single trait, the leaf shape.

Is it not known on how many chromosomes genes for leaf shape are located? Mind taking an educated guess?

[Sam_Skunkman]

Really have no idea and will not even try a guess. you said: "I don't think that is true in all polyhybrids... and I don't think that will hold true for future modern bred cultivars. The leaf shape of the latter will depend on the desired structure for farmers". I agree with you. Cultivars are based on drug content, WLD & NLD, Vs WLH & NLD, as well as leaf shape, but I agree that Cannabinoid, terpene profiles as well as terpene class, will all play a part. Type 1 THC, type 2 THC/CBD, type 3 CBD, type 4, type 5, type 6? for CBC and CBG as well as THCV and the other propyl homalogs, and maybe for zero Cannabinoids will also have a Chemotype. Maybe even some of the lesser known plant made Cannabinoids like CBT?
Sativa's as NLH and WLH, Indica's like WLD and NLD. I would add flowering dates at a given latitude, as well as the length of flowering outdoors or indoors.
-SamS

[Sam_Skunkman]

If you have trouble with sci-hub.io try .cc or .bz
-SamS

[Sam_Skunkman]

Beyond Cannabis: Plants and the Endocannabinoid System
Ethan B. Russo

Trends in Pharmacological Sciences

https://www.cell.com/trends/pharmacol...showall%3Dtrue

[Bubbleblower]

Originally Posted by Betterhaff

Hey Sam or anybody...do you have access to the full paper?

Evolution of the Cannabinoid and Terpene Content During the Growth of Cannabis sativa Plants from Different Chemotypes

It is interesting, you should harvest after 12 days.
No, but this is really interesting:

"the characteristic terpenes of chemotype I had high correlation coefficients with THCA and negative coefficients with CBDA."

[Betterhaff]

Thanks Bubbleblower...I used the Sci-hub site with the DOI to view it as Sam suggested. Haven't been able to find the last article he posted, might not be there yet.