[Sam_Skunkman]

https://www.cell.com/current-biology/...822(11)00771-8


Regenerant Arabidopsis Lineages Display a Distinct Genome-Wide Spectrum of Mutations Conferring Variant Phenotypes
Caifu Jiang, Aziz Mithani, Xiangchao Gan, Eric J. Belfield, John P. Klingler, Jian-Kang Zhu, Jiannis Ragoussis, Richard Mott, Nicholas P. Harberd
DOI: https://dx.doi.org/10.1016/j.cub.2011.07.002

Summary
Multicellular organisms can be regenerated from totipotent differentiated somatic cell or nuclear founders [ 1–3 ]. Organisms regenerated from clonally related isogenic founders might a priori have been expected to be phenotypically invariant. However, clonal regenerant animals display variant phenotypes caused by defective epigenetic reprogramming of gene expression [ 2 ], and clonal regenerant plants exhibit poorly understood heritable phenotypic (“somaclonal”) variation [ 4–7 ]. Here we show that somaclonal variation in regenerant Arabidopsis lineages is associated with genome-wide elevation in DNA sequence mutation rate. We also show that regenerant mutations comprise a distinctive molecular spectrum of base substitutions, insertions, and deletions that probably results from decreased DNA repair fidelity. Finally, we show that while regenerant base substitutions are a likely major genetic cause of the somaclonal variation of regenerant Arabidopsis lineages, transposon movement is unlikely to contribute substantially to that variation. We conclude that the phenotypic variation of regenerant plants, unlike that of regenerant animals, is substantially due to DNA sequence mutation.

[Sam_Skunkman]

.

[Skinny Leaf]

Originally Posted by oldchuck

"Shoots multiplied on the same medium for two sub-cultures were able to induce healthy roots within 4–6 weeks."

Doesn't seem all that impressive for some kind of super cloning technique.

That may seem like a long time for "traditional" cloning, but, when the starting material is only a leaf snippet, that may be fairly quick. The main point of the paper was to not transfer the generated shoot from one medium to another to get the shoot to root. The mT was effective in their trial at eliminating a second medium in the course of plant regeneration.

The maximized regeneration protocol using mT is thus effective and safe for large scale production of true to type C. sativa plants.

So, if one only has to put a plant snippet in one test tube to get a rooted clone then the work is cut in half. If I was doing mass production this would be the way to go for new plantlets. One mother plant and thousands of test tubes. Way smaller foot print.

That paper I am interested in.

[Skinny Leaf]

Originally Posted by Sam_Skunkman

As soon as I find a link for the paper I will post it. If anyone finds one please post. Meanwhile, I did ask the author for the paper, so I can read it.
Go here: https://sci-hub.io/
-SamS

When you get the paper can you let us know if the root or the shoot came first. It seems the way the paper reads the shoot came first then roots. But, its not really clear.

[Skinny Leaf]

Well, did you get the paper Sam?

I found this in one of the articles you posted.

https://www.sciencedirect.com/science...90536X14000525

The fourth constituent is tetrahydrocannabinol also known as Δ9THC which is considered the main or principal psychoactive constituent of the cannabis plant. It is classified as schedule 1 under the convention on psychotropic substances. It is available in a synthetic form as well, its brand name is Marinol. It is a toxic component and can cause death due to over dosing.

Are they saying Marinol is fatal in an overdose case or THC? WTF

[Sam_Skunkman]

The paper is from Pakistan, get it??? That is why THC is deadly...
-SamS

Originally Posted by Skinny Leaf

Well, did you get the paper Sam?

I found this in one of the articles you posted.

https://www.sciencedirect.com/science...90536X14000525

The fourth constituent is tetrahydrocannabinol also known as Δ9THC which is considered the main or principal psychoactive constituent of the cannabis plant. It is classified as schedule 1 under the convention on psychotropic substances. It is available in a synthetic form as well, its brand name is Marinol. It is a toxic component and can cause death due to over dosing.

Are they saying Marinol is fatal in an overdose case or THC? WTF

[Skinny Leaf]

That's cool I can get the paper to find my answer. Yes I would die if I went to Pakistan to acquire THC.

[Skinny Leaf]

License date
Apr 17, 2016

Licensed Content Publisher
Elsevier

Licensed Content Publication
Journal of Applied Research on Medicinal and Aromatic Plants

Licensed Content Title
In vitro mass propagation of Cannabis sativa L.: A protocol refinement using novel aromatic cytokinin meta-topolin and the assessment of eco-physiological, biochemical and genetic fidelity of micropropagated plants

Licensed Content Author
Hemant Lata,Suman Chandra,Natascha Techen,Ikhlas A. Khan,Mahmoud A. ElSohly

Licensed Content Date
March 2016

Licensed Content Volume Number
3

Licensed Content Issue Number
1

Type of Use
Content Purchase

Portion
Full Article



Looks like I am still alive after purchasing the paper. Didn't even have to travel to Pakistan.

[Sam_Skunkman]

You can get most science papers for free at:

sci-hub.io
sci-hub.cc

[Sam_Skunkman]

Very good paper. One of the best recently. Chimera bred many of the plants used.
-SamS

https://www.ncbi.nlm.nih.gov/pubmed/26651562


J AOAC Int. 2015 Nov-Dec;98(6):1503-22. doi: 10.5740/jaoacint.15-116.
Development and Validation of a Reliable and Robust Method for the Analysis of Cannabinoids and Terpenes in Cannabis.
Giese MW1, Lewis MA, Giese L, Smith KM.

Abstract
The requirements for an acceptable cannabis assay have changed dramatically over the years resulting in a large number of laboratories using a diverse array of analytical methodologies that have not been properly validated. Due to the lack of sufficiently validated methods, we conducted a single- laboratory validation study for the determination of cannabinoids and terpenes in a variety of commonly occurring cultivars. The procedure involves high- throughput homogenization to prepare sample extract, which is then profiled for cannabinoids and terpenes by HPLC-diode array detector and GC-flame ionization detector, respectively. Spike recovery studies for terpenes in the range of 0.03-1.5% were carried out with analytical standards, while recovery studies for Δ9-tetrahydrocannabinolic acid, cannabidiolic acid, Δ9-tetrahydrocannabivarinic acid, and cannabigerolic acid and their neutral counterparts in the range of 0.3-35% were carried out using cannabis extracts. In general, accuracy at all levels was within 5%, and RSDs were less than 3%. The interday and intraday repeatabilities of the procedure were evaluated with five different cultivars of varying chemotype, again resulting in acceptable RSDs. As an example of the application of this assay, it was used to illustrate the variability seen in cannabis coming from very advanced indoor cultivation operations.