Register ICMag Forum Menu Features Search Today's Posts Mark Forums Read
You are viewing our:
in:
Forums > Marijuana Growing > Marijuana Strains and Breeding > CANNABIS DNA PROJECT

Thread Title Search
Click for Cannapot - buy cannabis seeds
Thread Closed
CANNABIS DNA PROJECT Thread Tools Search this Thread
Old 11-19-2014, 05:48 AM #1
Sam_Skunkman
"RESIN BREEDER"

Sam_Skunkman's Avatar

Join Date: Mar 2004
Posts: 4,516
Sam_Skunkman has a brilliant futureSam_Skunkman has a brilliant futureSam_Skunkman has a brilliant futureSam_Skunkman has a brilliant futureSam_Skunkman has a brilliant futureSam_Skunkman has a brilliant futureSam_Skunkman has a brilliant futureSam_Skunkman has a brilliant futureSam_Skunkman has a brilliant futureSam_Skunkman has a brilliant futureSam_Skunkman has a brilliant future
CANNABIS DNA PROJECT

Cannabis Colleagues,

Greetings from the International Hemp Association in Amsterdam. We are writing to tell you about an exciting research program using modern DNA analysis to build a family tree for Cannabis, and to request your participation.

We are collaborating with a US-based group working on a definitive large-scale phylogenetic study of the evolution of Cannabis. Key researchers include Mowgli Holmes, the chief scientific officer at Phylos Bioscience, Rob DeSalle, a professor of evolutionary biology at Columbia University and curator at the American Museum of Natural History.

Presently, the team is building a high-resolution map of the Cannabis genome, based on a modern hybrid THC/CBD strain, using PacBio long-read Next-Gen Sequencing (NGS). The map will serve as a reference key for analysis of thousands of other accessions using an SNP (Single Nucleotide Polymorphism) NGS protocol called GBS (Genotyping By Sequencing) allowing high-resolution characterization of each accession.

So far, the group has sequenced over 400 drug-type Cannabis samples. DNA extraction equipment is installed in nine Cannabis testing labs in six US states, and we continue to gather modern hybrid drug varieties. In the next month the group will finish collecting and sequencing our first 1000 samples. In order to collect data from ancient seeds the group is modifying protocols used for sequencing fragmented Pleistocene DNA samples, developing protocols to work with single seeds, and adding a Whole Genome Amplification step to increase DNA yield.

In two or three months we hope to have a provisional relationship map worked out that we can put on the web and will let us ID modern strains and hopefully at least test theories concerning the evolution of Cannabis.

Within six months we will analyze additional modern cultivars plus traditional landraces and possibly herbarium sheets and archeological materials. From there on we will add to the live database which will continue to grow as more samples are received although the architecture should not change. Genome data will be studied via network theory to address the issues of hybridization and reticulation in the phylogeny.

Cannabis is an incredibly varied genus made up of a myriad of local landrace varieties and modern cultivars as well as their feral and wild relatives. This research will generate a huge amount of sequence data and unique SNPs spread over many thousands of samples, and we feel confident we can resolve the evolution of Cannabis under domestication.

To further our study and create as complete an evolutionary tree as possible we need seeds collected overseas (or reproduced domestically) and have not been interbred with modern drug hybrids. We are interested in ALL Cannabis whether grown for fiber, seed or drug production as well as feral and wild populations. We can now collect sufficient high-quality DNA for analysis from a single seed. And there is no requirement to grow the seeds, so we can also use dead seeds. Many conscious travelers, marijuana users and growers collected seeds that they never got around to sowing, and now years later they are dead. Dead seeds are useless to growers and breeders, but they still contain valuable genetic information that can provide us with deeper insights into Cannabis’s evolution. It is also legal for us to send dead non-viable seeds to our lab in the USA by post.

If you have any seeds you feel may be of interest (living or dead) and want to contribute to this fascinating research feel free to contact us, just PM SamS at IC. We will provide a Netherlands PO Box for you to send the seed samples to us and we will provide an optional questionnaire about each batch of seeds. Batches can range from just a single to 25+ seeds.

As research progresses we will share data with you about any accessions you provide. Upon completion the results will change how we all look at Cannabis, and we will better understand the heritage of modern Cannabis cultivars. Growers will be able to see how their varieties (as well as traditional landraces sent by contributors) fit into the big picture – to determine the landrace origins of modern hybrids (ex., Jamaican, Mexican, Colombian, etc.) and explore deeper evolutionary relationships. Fascinating, eh?
If you have any questions please feel free to contact us.

All the best,

Rob Clarke and Sam Skunkman



Many people have asked us questions about our projects. The questions below are the ones we hear again and again. We want our work to be good for everyone involved, and we intend to be transparent about how and why we’re doing it.


PHYLOS BIOSCIENCE DNA PROJECT FAQ

Why is Phylos sequencing the DNA of all these Cannabis strains?

A few reasons. First we have some scientific questions we just want answered. We want to know how Cannabis has evolved, what its history was like, and how it has co-evolved with humans. And we want to know what domestication does to the shape of evolution.

We also want to understand today’s crazy mix of hybrid strains. We want to know where they came from, why they’re so different, and what makes each one unique.

But the reason that will probably affect most people is that we want to change the Cannabis industry into a modern, legitimate marketplace, where people actually know what they’re getting. We think this is the only way.


Will Phylos make and sell genetically modified Cannabis?

No. Absolutely not.


Will someone else make and sell genetically modified Cannabis?

Maybe. But it won’t be us. And it won’t make much sense to do it, because it is so easy to create amazing strains of Cannabis using traditional breeding techniques.


Will Phylos patent the strains that are submitted? Doesn’t sequencing the DNA of something let you control it or patent it?

We won’t patent the strains that are submitted. We don’t want to, and we couldn’t. Here are a few important facts about patents and plants:

* Sequencing the DNA of something does NOT let you patent it. In fact, after the court cases <https://www.supremecourt.gov/opinions/12pdf/12-398_1b7d.pdf>Assoc. for Molecular Pathology v. Myriad Genetics and <https://www.supremecourt.gov/opinions/11pdf/10-1150.pdf>Mayo Collaborative Services v. Prometheus Laboratories, Inc., it is no longer possible to patent naturally occurring DNA sequences at all.

* Cannabis strains cannot be patented, at least not now. To apply for a plant patent you have to declare that you successfully grew your new Cannabis variety somewhere on U.S. soil. The patent office will not grant claims that rest on violations of federal law.

* Intellectual property protection over a plant variety can also be obtained through the USDA Plant Variety Protection Office. But only for plants on their official list. Cannabis is not on that list, and won’t be until it is federally legal.

* Only things that are NEW can be patented. Once something is public and known about, it’s too late. Once something has been sold commercially, it’s too late. Even if Cannabis strains could be patented, all the existing strains are now in the public domain, and will stay there forever. Which is a good thing.


But doesn’t Monsanto want to patent all the Cannabis strains in the world and then make it illegal for me to grow any of them?

Probably. But existing strains can’t be patented.



What will Phylos do with the information it collects from sequencing the DNA of different Cannabis varieties?

We will use it to construct a map of all the different strains. We will publish this and make the data freely available to the research community. The individual data from each sample will also be freely available to the person who submitted it.

Last edited by Sam_Skunkman; 12-13-2014 at 07:09 AM..
Sam_Skunkman is offline


38 members found this post helpful.
Old 11-19-2014, 05:59 AM #2
harry74
Banned

Join Date: Nov 2011
Location: The Frontline
Posts: 1,539
harry74 is a name known to allharry74 is a name known to allharry74 is a name known to allharry74 is a name known to allharry74 is a name known to allharry74 is a name known to allharry74 is a name known to allharry74 is a name known to allharry74 is a name known to allharry74 is a name known to allharry74 is a name known to all
I think was my idea but I haven´t got the logistics so is great to hear that someone is doing this.

The portuguese were for long time in India around Goa.
https://en.wikipedia.org/wiki/Portuguese_India

I don´t know if Indian genetics could somehow arrive to Brazil but the link is there.

¿Could we take some of that brazilian genetics and trough DNA testing trace their origins?

No idea really, but would be interesting.

A worldwide Cannabis DNA library?
https://www.icmag.com/ic/showthread.php?t=290988
harry74 is offline


1 members found this post helpful.
Old 11-19-2014, 08:30 AM #3
stix~
Member

Join Date: Nov 2013
Posts: 308
stix~ is just really nicestix~ is just really nicestix~ is just really nicestix~ is just really nicestix~ is just really nicestix~ is just really nicestix~ is just really nicestix~ is just really nicestix~ is just really nicestix~ is just really nicestix~ is just really nice
max respect Samskunk .. amazingly interesting proyect .. best o luck!!! wish ya needed a helper jeje

i doubt i can contribute any helpful hybrids....however , i'm looking forward to sendin all the landraces in my seedstash.... New Guinea, thai79 , mexican oaxacan , Costa.rican Sativa , Colorado p.bud , & a couple more

good vibes!!!
stix~ is offline


Old 11-19-2014, 12:34 PM #4
CosmicGiggle
Senior Member

Join Date: Oct 2006
Posts: 1,310
CosmicGiggle has a reputation beyond reputeCosmicGiggle has a reputation beyond reputeCosmicGiggle has a reputation beyond reputeCosmicGiggle has a reputation beyond reputeCosmicGiggle has a reputation beyond reputeCosmicGiggle has a reputation beyond reputeCosmicGiggle has a reputation beyond reputeCosmicGiggle has a reputation beyond reputeCosmicGiggle has a reputation beyond reputeCosmicGiggle has a reputation beyond reputeCosmicGiggle has a reputation beyond repute
Smile

Quote:
Originally Posted by Sam_Skunkman View Post
Presently, the team is building a high-resolution map of the Cannabis genome, based on a modern hybrid THC/CBD strain,.....
..... would that be Skunk #1?
CosmicGiggle is offline


Old 11-19-2014, 01:24 PM #5
Mustafunk
Heirloom Grower

Mustafunk's Avatar

Join Date: Aug 2007
Location: 42N
Posts: 1,333
Mustafunk has much to be proud ofMustafunk has much to be proud ofMustafunk has much to be proud ofMustafunk has much to be proud ofMustafunk has much to be proud ofMustafunk has much to be proud ofMustafunk has much to be proud ofMustafunk has much to be proud ofMustafunk has much to be proud ofMustafunk has much to be proud ofMustafunk has much to be proud of
Fantastic project, I'm sure many members of the community would love to take part and support this. The results of such an intensive research will definitely help us to gain a better understanding of this plant and its evolution, providing answers, that's what it is all about. I'm looking forward to see this happening.

Best of luck with the project.
Vibes.

Last edited by Mustafunk; 11-19-2014 at 02:51 PM..
Mustafunk is offline


1 members found this post helpful.
Old 11-19-2014, 06:22 PM #6
Betterhaff
Senior Member

Join Date: Jun 2013
Posts: 2,088
Betterhaff has much to be proud ofBetterhaff has much to be proud ofBetterhaff has much to be proud ofBetterhaff has much to be proud ofBetterhaff has much to be proud ofBetterhaff has much to be proud ofBetterhaff has much to be proud ofBetterhaff has much to be proud ofBetterhaff has much to be proud ofBetterhaff has much to be proud ofBetterhaff has much to be proud of
Quote:
Originally Posted by Sam_Skunkman View Post
Presently, the team is building a high-resolution map of the Cannabis genome, based on a modern hybrid THC/CBD strain, using PacBio long-read Next-Gen Sequencing (NGS). The map will serve as a reference key for analysis of thousands of other accessions using an SNP (Single Nucleotide Polymorphism) NGS protocol called GBS (Genotyping By Sequencing) allowing high-resolution characterization of each accession.
I’m curious as to what strain is the base line and why? I’m sure it will come out as the research develops but just trying to rap my head around what would be chosen. Very interesting project and long overdue.
Betterhaff is offline


Old 11-19-2014, 06:28 PM #7
chefbudz
Member

chefbudz's Avatar

Join Date: Apr 2014
Posts: 215
chefbudz has a spectacular aura aboutchefbudz has a spectacular aura aboutchefbudz has a spectacular aura aboutchefbudz has a spectacular aura about
its such an amazing time in the evolution of cannabis
chefbudz is offline


Old 11-19-2014, 06:29 PM #8
high life 45
Seen your Member?

high life 45's Avatar

Join Date: Mar 2012
Location: Cascadia 541
Posts: 1,997
high life 45 is a name known to allhigh life 45 is a name known to allhigh life 45 is a name known to allhigh life 45 is a name known to allhigh life 45 is a name known to allhigh life 45 is a name known to allhigh life 45 is a name known to allhigh life 45 is a name known to allhigh life 45 is a name known to allhigh life 45 is a name known to allhigh life 45 is a name known to all
I was waiting for this to happen! The genome of hops has been mapped out, so it was only a matter of time for cannabis to follow. Be interesting to see the genetic foundation of OGs
__________________
Juice cannabis and eat salad everyday.

Food is medicine, medicine is food.



high life 45 is offline


Old 11-20-2014, 02:01 AM #9
PrimalHaze
Member

Join Date: Aug 2014
Posts: 461
PrimalHaze is a name known to allPrimalHaze is a name known to allPrimalHaze is a name known to allPrimalHaze is a name known to allPrimalHaze is a name known to allPrimalHaze is a name known to allPrimalHaze is a name known to allPrimalHaze is a name known to allPrimalHaze is a name known to allPrimalHaze is a name known to allPrimalHaze is a name known to all
Lightbulb

Quote:
Originally Posted by Sam_Skunkman View Post
Cannabis Colleagues,

If you have any seeds you feel may be of interest (living or dead) and want to contribute to this fascinating research feel free to contact us, just PM SamS at IC. We will provide a Netherlands PO Box for you to send the seed samples to us and we will provide an optional questionnaire about each batch of seeds. Batches can range from just a single to 25+ seeds.
Howdy Sam,

I'm excited to see your findings. Mapping the family tree and evolution of cannabis will be a revealing project.

I know you say dead seeds are fine, but this seems like a prime opportunity to pool old genetics. Do you have any special tech to revive the oldest of seeds so you can germinate and self the lines?

I wish you could've had a chance to revive some of those 2,700 yr. old Gushi seeds. Jurrassic park style.

Thanks!
-pH
PrimalHaze is offline


1 members found this post helpful.
Old 11-20-2014, 03:10 AM #10
oldchuck
Member

Join Date: Sep 2010
Posts: 978
oldchuck is a name known to alloldchuck is a name known to alloldchuck is a name known to alloldchuck is a name known to alloldchuck is a name known to alloldchuck is a name known to alloldchuck is a name known to alloldchuck is a name known to alloldchuck is a name known to alloldchuck is a name known to alloldchuck is a name known to all
Great project. Looking forward to monitoring your progress.
oldchuck is offline


Thread Closed

Thread Tools Search this Thread
Search this Thread:

Advanced Search

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off


All times are GMT +2. The time now is 12:25 AM.


Click for Weed Seed Shop


This site is for educational and entertainment purposes only.
You must be of legal age to view ICmag and participate here.
All postings are the responsibility of their authors.
Powered by: vBulletin Copyright ©2000 - 2017, Jelsoft Enterprises Ltd.