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Old 03-21-2015, 09:21 PM #41
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Stop it now... your'e just making a fool of yourself. Do you know anything about what you're copy/pasting????
Worst of all he ruins every single interesting thread.
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Old 03-21-2015, 11:59 PM #42
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Interesting question, how does "...epigenetic regulation link[s] plant nutrients with mono-/sesquiterpene biosynthesis."



I do believe there are some labs using NMR for cannabinoid detection.

Any specific questions?

-----

Regarding terpenoid production. Yes, it appears that all conditions the same, even within a single "table" in a room one will have differences, all based on matters like where your "wind" was coming from and where your "water" source was coming from.

In nature, one will find the same 'phenomena' occur. Yes, terpenes do vary in a direct response to environmental conditions such as nutrient differences, naturally.

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Old 03-22-2015, 12:17 AM #43
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I think that it is well known that the terpene profile of the same clone can be altered (%'s of each terpene in the profile), I thought what was more interesting is if it just the terpene profile that is altered, or if any new terpenes are found because of nutrients or the environment. I doubt the later as terpene production is genetically controlled.
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Old 03-22-2015, 12:28 AM #44
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Originally Posted by MJPassion View Post
I had a thought pass through my cranium earlier and it made me wonder...

Do cannabis terpin profiles change with changes in nutrients used to grow the plants?
only been testing with the cheap sensing equipment i shipped with. but i'd definitely say the nutrients used in whatever ratios effect the final product.

i'm sure that's the reason organic buds turn out better. plant takes what it needs from an abundant pool of sources vs chemical ferts which only supply prescribed ratios of certain elements.





chemicals




organics
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Old 03-22-2015, 12:34 AM #45
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Originally Posted by Only Ornamental View Post

That's the point I wonder about: What I've seen so far is that it works in theory but the applicability for a specific sample has to be shown first and the way you put it, he hasn't tested cannabis either.

Thanks for elaborating!
He has tested Cannabis with proton NMR and continues to do so, and several other Cannabis labs have NMR available and have started using it. Most are connected to a University, maybe the recent handful of newer, smaller, cheaper, bench top NMRs will allow wider use if NMR? Or at least understanding of any potential value for Cannabis analysis.
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Old 03-22-2015, 12:48 AM #46
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Plants can smell other plants. They react and sense chemicals in the air. Healthy plants do this more, they smell more. I'd say micro nutrients are what are used for these plant functions, which you can't have a plant without the basics. Plants also suck up what's in the water and makes it through cell walls. So a lot of chemicals can influence the smell and taste if enough is absorbed. Some people notice and can tell what a bud has been fed. A double blind study would be cool?

I've noticed a lot of differences between chem grown, hydro with pure blend pro, red wood mulch, native soils, and native soils with epsoma top dressing. I find red wood mulch exposed buds are acrid. Pure blend plants where fairly good and natural with a flush, but sorta bland without. Sweetness are bummers in my experience with b'cuzz, overly fake and sweet. Native soil with a higher ph got the "cream" taste and smell (heavenly).
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Old 03-22-2015, 08:51 AM #47
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Honestly, that epigenetic thing is, at least for the time being, as useful for a grower as a radio telescope in his bedroom (unless ET is the last hope of saving your crops)... I was just giving a certain person the possibility to work his way up the intellectual pecking order. Reminds me of which: thanks BB for joining, the competition is sooo much more fun with you around!


Back to topic...
Things we haven't considered are yet:
Many of our plants grow at the upper biological limit. Essential nutrients are often in abundance and the regulatory mechanisms for volatile terpenes can function independently of regulatory mechanisms for nutrient uptake/transport. Slight fluctuation will not be registered unless it's that of the weakest link.
On the other hand, growing on deficient soil or under pest challenge makes the former depending on the latter. Nutrient metabolism is diverted in order to reduce the threat (grow roots, fight diseases, survive drought). In some cases, terpenoid synthesis is hampered due limited supply and because it just use up scarce energy, in other cases it is boosted because some terpenes are useful to fight pests which cause the nutrient shortage. Many aromatic herbs produce, though a lower biomass, a higher concentration of volatiles under suboptimal/stressful conditions. Such stress is often absent in our plants and that's where the application of JA, SA, chitosan, and so on come into play. We want full growth and a certain stress response too (which is completely unnatural).

When talking about nutrients, do we only include essential nutrients (inorganic salts, O2, H2O, and CO2) or also 'metabolic' nutrients such as carbohydrates, amino acids, and lipids?
Unlike the former, the latter are also directly involved in pest sensing and plant defence and hence volatile terpene production. Plants have an extensive sugar signalling network which, at least in theory and in thale cress, affects secondary metabolite production. Amino acids such as glutamate, proline, glycine, and aspartic acid are in the middle of stress responses and herbivore sensing etc. Lipids play a crucial role as precursors for signalling molecules which are also linked to secondary metabolite production.
Some of these 'nutrients' are used as additives to influence plant metabolism. Though often, quite high concentrations are needed and are most effective on seedlings and young plants whereas they serve mainly as microbial food when given to adult plants.
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Old 03-22-2015, 12:45 PM #48
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if terpene production is influenced by... genetics and enviroment.
then nutrients will effect terpene production. then predators will influence terpene production.

what are specific variables in an enviroment that can cause higher linalool levels or myrcene levels?

would introducing different natural pest predators produce less pungent varieties?

follow me on this one... if scent is viewed like sound then what are higher note scents and lower note scents. Even better, ultra violet scents or sub harmonic scents. Meaning, scents we can't pick up, but other creatures and instruments may pick up (carbon monoxide meters).

great read everybody!
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Old 03-23-2015, 02:14 AM #49
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Quote:
Originally Posted by curbiechris View Post
if terpene production is influenced by... genetics and enviroment.
then nutrients will effect terpene production. then predators will influence terpene production.

what are specific variables in an enviroment that can cause higher linalool levels or myrcene levels?

would introducing different natural pest predators produce less pungent varieties?

follow me on this one... if scent is viewed like sound then what are higher note scents and lower note scents. Even better, ultra violet scents or sub harmonic scents. Meaning, scents we can't pick up, but other creatures and instruments may pick up (carbon monoxide meters).

great read everybody!
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Old 03-23-2015, 03:07 AM #50
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been learnin from you fools for the past 15 years
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