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Old 03-21-2015, 02:43 PM #31
Only Ornamental
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...in the gaseous phase then they get separated by their affinity to the stationary phase which is the column they travel through.On their way out they are broken down into fragments and the ions are identified by their mass to charge ratio.
Guess you just mixed GC and MS up a little bit .
Besides, unlike other chromatographic methods it's usually (with exceptions) not primarily the affinity for the column but the difference in gas pressure that separates sample constituents...
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Old 03-21-2015, 02:52 PM #32
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I don't have any science to back it up,, but I have grown my house strain with at least 8 different nute lines,, and I have gotten 8 different flavors..so I would say,, yes,, dif nutes can change things..
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Old 03-21-2015, 03:22 PM #33
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GC is a separation method and MS is a qualitative/quantitative identification method. Think of these things as modules different techniques get combined into one same piece of equipment. First you separate then you identify.
Acridlab…how would you rate those differences??Subtle...like in a bit more/less piney ext..….or drastic like it went from pine to mango? I doubt the latter was the case.Were you growing that strain from seed or clone>Where the clones from the same mother? I am pretty sure that if you grew a strain from clones, from the same mother, under homogeneous environmental conditions, with different nutrient concoctions ( assuming that they all contain the necessary micro/macro nutrients) you would only get different ratios of the same terpenoids, not completely different terpenoids on each.
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Old 03-21-2015, 03:30 PM #34
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Originally Posted by Only Ornamental View Post
Guess you just mixed GC and MS up a little bit .
not primarily the affinity for the column but the difference in gas pressure that separates sample constituents...
Ok,teach me….difference in pressure of what gas????
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Old 03-21-2015, 03:58 PM #35
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Quote:
Originally Posted by tropicanna View Post
GC is a separation method and MS is a qualitative/quantitative identification method. Think of these things as modules different techniques get combined into one same piece of equipment. First you separate then you identify.
Acridlab…how would you rate those differences??Subtle...like in a bit more/less piney ext..….or drastic like it went from pine to mango? I doubt the latter was the case.Were you growing that strain from seed or clone>Where the clones from the same mother? I am pretty sure that if you grew a strain from clones, from the same mother, under homogeneous environmental conditions, with different nutrient concoctions ( assuming that they all contain the necessary micro/macro nutrients) you would only get different ratios of the same terpenoids, not completely different terpenoids on each.
oh,, all clones from same mothers, too


I would say subtle for the most part... For instance,, my og18 with advanced, tastes super piney, while h&g makes it taste more chemmy funk... And my sour kush is straight gasoline/green apple with advanced,and just green apple/cleaner with h&g.. The only time things changed a lot, was when I tried gh Lucas with flora nectar.. Made my sour smelll and taste like just like the flora nectar".. The berry one..
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Old 03-21-2015, 04:31 PM #36
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Ok,teach me….difference in pressure of what gas????
Vapour pressure of the sample . It's just an equilibrium between adhesion/desorption and usually not an actual interaction with the stationary phase as seen in liquid chromatography between sample and silica.
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Old 03-21-2015, 05:10 PM #37
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Yeah,those are very reasonable variations.Chimera summed it up by using the term epigenetics...most living things tend to be ruled by pretty complex systems with lots of modularity and cascading mechanisms that give way to lots of variation and diversity as means to achieve evolution.
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Old 03-21-2015, 05:26 PM #38
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Vapour pressure of the sample . It's just an equilibrium between adhesion/desorption and usually not an actual interaction with the stationary phase as seen in liquid chromatography between sample and silica.
Stop it now... your'e just making a fool of yourself. Do you know anything about what you're copy/pasting????
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Old 03-21-2015, 06:48 PM #39
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FWIW, assuming I were a granny sitting in her rocking chair in a basement with the Encyclopaedia Britannica on her lap copy-pasting stuff, at least I'd be doing it correctly and not stuff like
Quote:
GC conditions.They boil off and evaporate and in the gaseous phase then they get separated by their affinity to the stationary phase which is the column they travel through.On their way out they are broken down into fragments and the ions are identified by their mass to charge ratio
. A GC hasn't m/z as readout. Sure, you can talk your way out by ignoring that we were talking about GC-FID and saying that in your case it's a GC-MS...
Small advice: Don't try to outsmart me, you'd utterly fail (cause even I have difficulties doing so ROFL).

And now, please get back to topic!
You could for example prove that you're actually able to read the instruction manual for your brain by explaining to the users here how epigenetic regulation links plant nutrients with mono-/sesquiterpene biosynthesis.
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Old 03-21-2015, 08:00 PM #40
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Just read before you copy/paste cause if you look back through my posts I've not mentioned anything else other than GC-MS, which is the standard for organic molecules. You show your ignorance just mentioning FID for terpene analysis. Where did you get your Ph.D., did it come with your happy meal? I won't waste any more of my time with you dude, I just hope that people that are interested in learning about these subjects realize that listening to you they might end regurgitating a lot of nonsense.
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