[genedigger]

sulfer can affect the terpene profile?wow!i use a liquid shit mixture on my plants and i had some in a container that i forgot about for a couple days,and when i went to use it it had a wicked sulfer smell to it.i was nevous to water with it so i only used it on 2 plants.damn did those plants turn out stinky.i was afraid it was going to kill them but they turned out really good.i have always heard not to store teas and nuites for more than a day but it was at least 2 or 3 sitting in the jug festering.

[Only Ornamental]

Originally Posted by FatherEarth

... Proton Nuclear magnetic resonance appears to be the way to test for terpene profiles without using heat...chromatography uses heat thus alters the true profile...

1H-NMR is a method of detection (structure determination) whereas chromatography is a family of methods for separation. In some cases it is indeed possible to determine the absolute amounts of constituents in a mixture. Usually it's only relative amounts and may not work well with plant extracts (I see problems with a bunch of structurally related terpenes). As the sample has to be in a deuterated solvent, some sample preparation is usually needed which, in case of volatile constituents, is no good.
The only chromatography which needs high temperatures is GC and sometimes MS (though that doesn't disturb in the latter case), all the others (TLC, open column, HPLC, CCC etc.) can work with ambient temps.
The 'best' method, if there is just one, would be UPLC-MS (TOF or MS/MS or some other fancy shmancy stuff) or better put a split in between and pump a fraction into a 1 gigahertz cryo-capillary NMR (1H and 13C). That way, you get directly the structures of all constituents as well .

[Only Ornamental]

Double post, sorry...

[MJPassion]

Thank yall for your answers.
K+ to all

One day... When I have the capacity... I'd like to have some samples tested.


One thing I've seen common with all those frost boosters...
KELP EXTRACTS.

[tropicanna]

Terpenes are organic molecules, specifically hydrocarbons.These are derived biosynthetic ally from isoprene units ( 2-methyl-1,3-butadiene) who's molecular formula is C5 H10.These molecules are the result of enzymatic mechanisms and the enzymes responsible are a product of particular genes being expressed. In other words ITS ALL IN THE GENES!! You can't make an orange taste/smell like a strawberry no matter what nutrient brand or concoction you decide to use. Sure, environmental factors come into play, but these factors modulate gene expression or enzymatic pathways not the smell/aroma directly.Phosphorus has more to do with the terpene profile and production of cannabis than sulfur ever would as their precursor is geranyl pyrophosphate.

[Bubbleblower]

Originally Posted by tropicanna

You can't make an orange taste/smell like a strawberry no matter what nutrient brand or concoction you decide to use.

Cannabis on the other hand is a different story, it's the master of copying code.
All we need are the right sequences.

[genedigger]

plants can be affected by whats grown near them.grow a tomato plant next to basil or do basil and peppers theres a difference.the flavor and more importantly the aroma will be increased,and imo they take on the essence of one another.theres many other companion plantings that will do this.mint under apple trees or borage under them.

[Sam_Skunkman]

Originally Posted by FatherEarth

Sulfur is a known input that influences terpene production...
I believe soil microbiology also plays a role in the full terpene profile. Not only that but a nutrient battery that allows the plant to utilize the nutrients it requires when it needs them most vs force fed when we think it needs whatever magic terpene potion available at the grow store. ... Proton Nuclear magnetic resonance appears to be the way to test for terpene profiles without using heat...chromatography uses heat thus alters the true profile...

Does that mean we need to stop smoking our Cannabis as that alters the terpenes? Or test the terpenes with GC which is more like what happens to Cannabis terpenes in a joint or pipe?
-SamS

[Sam_Skunkman]

I am not sure but I suspect only the profile is altered, not new or different terpenes with different nutrients or environments, just different %'s in the profile.
-SamS

Originally Posted by MJPassion

I had a thought pass through my cranium earlier and it made me wonder...

Do cannabis terpin profiles change with changes in nutrients used to grow the plants?

In other words,
How are terpin profiles effected by using different nutes?

I don't have the $, various nutes or the space to test this so I'm interested if anybody has done such experiments & had finished product tested via GCMS or other method to determin if there are any differences.

The proper experiment would use clones in the same environmentonly using different nutrients.

(If this is in the wrong section, i apologize in advanve & ask that it be placed in it's proper location.)

[tropicanna]

Good point there sam….vaping is a better method if you want to retain the integrity of flavor/aroma profile of your your buds, also supercritical CO2 extractions(eden labs) or winterizing (cold ethanol).These are volatile compounds with relatively low boiling points. Part of the reason you might want to use A/C with indoor high grade is to prevent the volatilization of these terpenoids as much as possible.