Tissue Culture - Breeder's Laboratory - International Cannagraphic Magazine Forums

NEGT1 · 10-28-2014, 02:10 PM

Are breeders pushing towards developments such as cell fusion and GMLs?

I would imagine it would help greatly in terms of recreating stable genes and expressions that are tagged to strains like OG Kush, Chemdog, UK Cheese, ect.

MJPassion · 10-28-2014, 05:02 PM

Interesting topic.

Does cell fusion occur in plants.
Assuming it does what would be the benefits of such in the breeding world?

NEGT1 · 10-28-2014, 05:10 PM

I don't know if it definitively can occur in cannabis but I presume so.

From what I understand it would allow breeders to bypass typical selective phenotype breeding for desired traits. Breeders could test genes of plants, pull out the specific traits they want and then fuse them with other traits from other phenos/varities/whatever you want to call them without having to physically test 1000 plants and then breed for the desired traits over 5-6 generations. Because with natural breeding, the breeder is still limited to the expressed traits in terms of selection. I believe tissue culture pretty much gets rid of that.

MJPassion · 10-28-2014, 05:51 PM

As I understand it, tissue culture is simply a method for keeping already selected plants around for future use.

Like clones but in a much more compact package.

GMLs? Please elaborate.

trichrider · 10-28-2014, 07:38 PM

...I wanna know too.
buddy interested in setting up a grow in co, needs thousands of plants.
this seems to be the easiest quickest method of production.

good topic.

purple_man · 10-28-2014, 10:06 PM

high fambz!

first protoplast fusion can be done with any cultivar, BUT the thing is, if you succeed in fusing them you recieve a chimera (a cultivar which holds both genotypes), hence no way to know how it is going to express at phenotype level (basicly the plant could be expressing mixed phenotypes throughout the plant, or the 2 different phenotypes on different branches etc...), ...

never heard of GML, if you mean GMO (genetically modified organism), no sense in doing so (at least right now), since there are no accurate techniques for transfering the target genes at neccessery target sites, plus gmos can't be sold/grown without special licenses, AND the most important part is, most of the so called breeders/seedbanks are a bunch of former hustlers/marketing guys n pollen chuckers, why waste millions on research and development when folks buy literally ANYTHING if the name sounds sexy....

blessss

deadkndys · 10-28-2014, 10:28 PM

How does tissue culture work with Cannabis? I know when using mushrooms it is a relativity easy process. Do they use agar or some other growing medium?

purple_man · 10-28-2014, 10:50 PM

similar to shrooms, but more complicated...

the biggest problem is the "sterile working technique" hence keepin your ish sterile...

basically you prepare a culture media (depending on what you plan doing, shoot formation, root formation, shoot multiplication etc...), add the nutrients, PGRs, agar some vitamins, etc...

yeah you use agar for "solidifing" the culture media for the "regular applications", but if you want a liquid culture (for future protoplast extraction), you don't use agar, instead you let the stemcell clump (callus) "swim" in the liquid media on a "rotator"...

blessss

trichrider · 10-28-2014, 11:12 PM

^^^similar to reproducing mycelial growth ( liquid culture).

just a cell or two transferred to a petri dish and supporting environment.

instead of a motherplant you'd keep petri dishes.

Dan Cooper · 10-29-2014, 01:03 AM

As soon as I get some extra elbow room I am going to put together an area to get into tissue culture. What do they call the cabinet? I wanna say flow bench but I know that's not it. Heck its time to go to storage and find that old reference manual on tissue culture

10-28-2014, 02:10 PM	#1
NEGT1 Member Join Date: Aug 2014 Posts: 363	Tissue Culture Are breeders pushing towards developments such as cell fusion and GMLs? I would imagine it would help greatly in terms of recreating stable genes and expressions that are tagged to strains like OG Kush, Chemdog, UK Cheese, ect.
	Quote

10-28-2014, 05:02 PM	#2
MJPassion Observer Join Date: Dec 2012 Location: uni-verse Posts: 5,584	Interesting topic. Does cell fusion occur in plants. Assuming it does what would be the benefits of such in the breeding world?
	Quote

10-28-2014, 05:10 PM	#3
NEGT1 Member Join Date: Aug 2014 Posts: 363	I don't know if it definitively can occur in cannabis but I presume so. From what I understand it would allow breeders to bypass typical selective phenotype breeding for desired traits. Breeders could test genes of plants, pull out the specific traits they want and then fuse them with other traits from other phenos/varities/whatever you want to call them without having to physically test 1000 plants and then breed for the desired traits over 5-6 generations. Because with natural breeding, the breeder is still limited to the expressed traits in terms of selection. I believe tissue culture pretty much gets rid of that.
	Quote

10-28-2014, 05:51 PM	#4
MJPassion Observer Join Date: Dec 2012 Location: uni-verse Posts: 5,584	As I understand it, tissue culture is simply a method for keeping already selected plants around for future use. Like clones but in a much more compact package. GMLs? Please elaborate.
	Quote 2 members found this post helpful.

10-28-2014, 07:38 PM	#5
trichrider THEORETICAL Join Date: Apr 2010 Location: between CB1 and the singularity. Posts: 7,046	...I wanna know too. buddy interested in setting up a grow in co, needs thousands of plants. this seems to be the easiest quickest method of production. good topic.
	Quote

10-28-2014, 10:06 PM	#6
purple_man Senior Member Join Date: Aug 2008 Location: up in smoke Posts: 1,521	high fambz! first protoplast fusion can be done with any cultivar, BUT the thing is, if you succeed in fusing them you recieve a chimera (a cultivar which holds both genotypes), hence no way to know how it is going to express at phenotype level (basicly the plant could be expressing mixed phenotypes throughout the plant, or the 2 different phenotypes on different branches etc...), ... never heard of GML, if you mean GMO (genetically modified organism), no sense in doing so (at least right now), since there are no accurate techniques for transfering the target genes at neccessery target sites, plus gmos can't be sold/grown without special licenses, AND the most important part is, most of the so called breeders/seedbanks are a bunch of former hustlers/marketing guys n pollen chuckers, why waste millions on research and development when folks buy literally ANYTHING if the name sounds sexy.... blessss
	Quote 5 members found this post helpful.

10-28-2014, 10:28 PM	#7
deadkndys Member Join Date: Apr 2013 Posts: 263	How does tissue culture work with Cannabis? I know when using mushrooms it is a relativity easy process. Do they use agar or some other growing medium?
	Quote 1 members found this post helpful.

10-28-2014, 10:50 PM	#8
purple_man Senior Member Join Date: Aug 2008 Location: up in smoke Posts: 1,521	similar to shrooms, but more complicated... the biggest problem is the "sterile working technique" hence keepin your ish sterile... basically you prepare a culture media (depending on what you plan doing, shoot formation, root formation, shoot multiplication etc...), add the nutrients, PGRs, agar some vitamins, etc... yeah you use agar for "solidifing" the culture media for the "regular applications", but if you want a liquid culture (for future protoplast extraction), you don't use agar, instead you let the stemcell clump (callus) "swim" in the liquid media on a "rotator"... blessss
	Quote 2 members found this post helpful.

10-28-2014, 11:12 PM	#9
trichrider THEORETICAL Join Date: Apr 2010 Location: between CB1 and the singularity. Posts: 7,046	^^^similar to reproducing mycelial growth ( liquid culture). just a cell or two transferred to a petri dish and supporting environment. instead of a motherplant you'd keep petri dishes.
	Quote 2 members found this post helpful.

10-29-2014, 01:03 AM	#10
Dan Cooper Newbie Join Date: Sep 2014 Location: Pacific North West Posts: 48	As soon as I get some extra elbow room I am going to put together an area to get into tissue culture. What do they call the cabinet? I wanna say flow bench but I know that's not it. Heck its time to go to storage and find that old reference manual on tissue culture
	Quote