[Chimera]

How to detect the presence of CBD, for home growers

https://www.madchat.fr/esprit/texture...nd_edition.pdf

Page # 127The Beam Test aka Beam's Test

If you read the section "Colorimetry" in the first paragraph on Page #127 you will see a simplified method for detecting CBD using a spot test called "the Beam test"

The needed reagents are:

5% KOH(Potassium hydroxide) in 95% ethanol
(5g KOH in 100 ml 95% ethanol)

Petroleum ether, Naptha or other organic solvent to remove the cannabinoids from a sample of your choosing.

Place 1-2 grams of your sample flowers in an oven until dry @ 150 degrees Celsius for 5 minutes. Extract in a small glass vessel (shot glass?) for 10 minutes in 10 ml of your Petroleum ether, other organic solvent, naptha, etc.

Filter and remove the leaf material using a coffee filter or similar, catch the liquid extract

Take 1 ml of the extract and dry on a white porcelain dish like an egg cup, saucer, or similar. (Gently heat the dish to evaporate the liquid phase of the extract). You will have a dish with remnants of resin leftover once the liquid has evaporated.

Place 5 drops of your 5% KOH in ethanol solution on your plate, and wait about 10 minutes. You will see a distinct violet/purple/pink color from samples containing CBD which could be Chemotype II or III plants - in the case of CBD Lullaby they will be only Type I and II plants- THC only plants (chemotype i) will not show this colour.

It's a relatively simple test and anyone can do it, you just need to order KOH from Amazon or your local soap making store and pick up a solvent from your local hardware store. The ethanol will probably be the most difficult thing to source, but it is available as "Everclear" or 190 proof ethyl alcohol from your local provincial or state run liquor store, depending on your location.

Hope that comes in handy....
-Chimera

[Kalbhairav]

Hey Brewery..

Congrats on being the first one to start a grow log of the new genetics..

Looking forward to your results..

[strass]

Thanks Chimera!

[icon]

subbed

[Betterhaff]

Originally Posted by Chimera

How to detect the presence of CBD, for home growers

Thank you for this.

[Kalbhairav]

Hey Brewery,

Looking forward to some more pics so we might follow along with you..

How are they coming along?

[Chimera]

Originally Posted by Betterhaff

Thank you for this.

You are very welcome. I felt it was my responsibility to provide a method to the community for simply detecting the presence of CBD in this seedlot, because unlike with other CBD lines available on the market, this seedline is made up of both Bd/Bt (Chemotype II- mixed CBD:THC) plants and Bt/Bt plants (Chemotype I, THC predominant)

I could have provided purely Bd/Bt populations as do our good friends at the CBD crew, but it will be revealed in due time why this choice was made- I assure you it was intentional.

Really looking forward to following your progress Brewery, thanks for all the updates.

Respectfuly,
-Chimera

[Kalbhairav]

Awesome progress Brewery..

Everything is looking good

So what size pots are you going for and what kind of mix you going to use?

[maryanne3087]

Anyone can acquire 95% ethanol, 90+% should all do the job.

Where it is legal to operate and own a still, one could simply distill vodka twice to obtain 90%+ ethanol, a simple still could be an oliver still which is just a pot, a wok or metal bowl for the condenser, and a collection vessel in the center where the alcohol will drip. Again only do this where it is legal!

99% iso is going to be a much more aggressive solvent vs the ethanol, the iso will have the tendency to dissolve more of the plant matter so it could throw this particular test off, or it might not, I don't know.

[CannaBen]

I bought about 50 of these seeds when they first dropped. I current;y have many many cultivars on the go looking for many keepers to do some work with for personal use.
I have popped only 5 so far. I got 4 females i believe heck maybe all 5 even. Have to double check when i am at The Lab next.
Most of them show "indica"/hybrid traits but 1 is pure "sativa" in leaf structure although it grows rather bushy if you top it ect.
Mine are further along, and we have already taken lots of clones of each one, but they are going to get flowered soon.
I was wondering why Chimera had not used 1 parent with Bt/Bt and the other with Bd/Bd to make a line with 100% of the progeny with CBD.
I was also wondering why he didn't use 2 parents both with Bt/Bd, to make a population with a 1:2:1 ratio of chemotypes 1,2 and 3.
My lord now that he said it is all for a very specific reason, i can barely contain my curiosity.

And that home test for detecting CBD presence.... killer info. I have a ton of CBD lines i am going through that i needed to determine which ones to send of for testing first.

Thanks a ton Chimera.. Man, you know your shit. I am new to your board and genetics but the little bit i have read from is massive indication of where you knowledge and experience is at. ok ok time to get off the nuts... hahaha