[omera1]

Hello,
Im dealing with a virus, maybe TMV. Looked around and i found something interessting:

Brassinolide (BL), considered to be the most important brassinosteroid (BR) and playing pivotal roles in the hormonal regulation of plant growth and development, was found to induce disease resistance in plants. To study the potentialities of BL activity on stress responding systems, we analyzed its ability to induce disease resistance in tobacco and rice plants. Wild-type tobacco treated with BL exhibited enhanced resistance to the viral pathogen tobacco mosaic virus (TMV), the bacterial pathogen Pseudomonas syringae pv. tabaci (Pst), and the fungal pathogen Oidium sp. The measurement of salicylic acid (SA) in wild-type plants treated with BL and the pathogen infection assays using NahG transgenic plants indicate that BL-induced resistance does not require SA biosynthesis. BL treatment did not induce either acidic or basic pathogenesis-related (PR) gene expression, suggesting that BL-induced resistance is distinct from systemic acquired resistance (SAR) and wound-inducible disease resistance. Analysis using brassinazole 2001, a specific inhibitor for BR biosynthesis, and the measurement of BRs in TMV-infected tobacco leaves indicate that steroid hormone-mediated disease resistance (BDR) plays part in defense response in tobacco. Simultaneous activation of SAR and BDR by SAR inducers and BL, respectively, exhibited additive protective effects against TMV and Pst, indicating that there is no cross-talk between SAR- and BDR-signaling pathway downstream of BL. In addition to the enhanced resistance to a broad range of diseases in tobacco, BL induced resistance in rice to rice blast and bacterial blight diseases caused by Magnaporthe grisea and Xanthomonas oryzae pv. oryzae, respectively. Our data suggest that BDR functions in the innate immunity system of higher plants including dicotyledonous and monocotyledonous species.

https://onlinelibrary.wiley.com/doi/1...3.01675.x/full

the interessting part is that Bras dont use the salyclyic acid pathways, but does induce sar.

So i made a spray:
100ppm Salycylic Acid
50ppm Thiamine(B1)
0.01ppm Bras.

lets see what happens.

[shaggyballs]

Omera you are on fire!!!!

Yes I also believe this is true of Brass.
I have been looking into Brass./SAR response for my SAR thread.
Its mode of action is very unusual.

Mix looks good!!!
If you want to mix it up a little?
Try harpin protein Especially for TMV!
https://www.ncbi.nlm.nih.gov/pubmed/21585268
I read somewhere a guy used it , yet he still had the virus but the plant showed no signs of having a virus.
https://grow-better.com/harpin-proteins/
You can get it in a tub but I think freshness is ultra important with this stuff!

Chitosan works also...But I believe you already know that.

[omera1]

thanks for your input!
i have some chitosan here, if my present spray did not help i will use it.
i stay away from that proteins. read somewhere here that it is a no-no. cant really remember. maybe i have to learn more about this harpin proteins.

i planed on spraying my new tria mix, and now this. have to use SA in veg which makes me unable to use the tria at this time -.- so its a good thing that i can use the bras to fight the virus. maybe i can get a little effect thats a bit similar to tria

[omera1]

what i can say from now, the spray is the shit.
looks like the turbo mode turned on

[shaggyballs]

Originally Posted by omera1

what i can say from now, the spray is the shit.
looks like the turbo mode turned on

Good morning Omera!

So are you confirming the benefits of your Brass mix spray on Disease resistance?

Or are you stating your new spray has a turbo effect on growth?
Or is it in fact doing both?
Either way this is fantastic news!

[omera1]

first thing i noticed is turbo growth. the growth from the infectet plant was very slow compared to the others. i saw noticeable strong growth 1-2 days after application. the others do the same. very effective in enhancing growth rate, thats for sure. let us wait a few days to see what happens to the virus (i have to say that im not 100% sure its a virus). i think i have to wait for 100% new developed leaves to tell for sure if there is a benefit from the spray

[aus2canibasiva]

I've been delving into the world of plant hormones and plant growth stimulants & have been enjoying reading your guys posts and others on ICMAG.
I have been seeing some great results after using Brassinolide 0.1% except instead of using the recommended 0.1 PPM by mistake I used 100 PPM with so far favorable results like Omera1 puts it it "first thing I noticed is turbo growth" that about sums it up.

My only worry at this stage is that there will be some bad effects later into flowering, ie' mutations, it was applied only a few days ago so we will see.

Does anyone know of any evidence that suggests or shows that large applications of Brassinolide has a detrimental effect on plant growth?

Happy Growing.

[shaggyballs]

Not positive but I think you will experience a big stretch.
Maybe try a little cytokinin to counteract/balance the brass.

Now this is a flyin by the seat of my pants guess but try 100 PPM BAP spray.

I noticed toxicity in the big leaves but cannot be sure it was BRASS. as I sprayed a few other things also!

[omera1]

oh fuck....100ppm is dam fucking high! i used 0.01ppm and there was for sure a stretch, not dramatic but too much imo. i think i will use 0.005 or even lower ppms next time. yeah try bap, lets hope the 100ppms are enough. i think its not enough, but better try lower ppms first. god luck man

edit:
i totally forgot that: after a while i can say fore sure my spray from the first post is a killer combo against TMV. never saw the virus again, completly gone and the infected-dwarf plant is now the big boy in the house. and that only after one single application. absolutly amazing. but again i think 0.005ppm will do the job also and reduce the stretch a bit.

[shaggyballs]

omera knows his stuff!!!!
I agree!!!
But I did not want to panic the guy .
Too late!!!!
But remember omera the amount is strain dependent!!
Stretchy strains need less.