[TickleMyBalls]

The high cbd strains on the market are crossed with industrial hemp. That's how they worked it into the lines. Fact. I've talked to breeders in the know.

[oldchuck]

That's very interesting, Herm, useful information. I've been wondering about those TLC kits. Does it seem pretty accurate? Can a technically challenged old guy figure out how to use it?

One other question, do you happen to know the variety of hemp you extracted.

The low yield doesn't bother me. All you need is more raw material.

[herm@n]

Originally Posted by oldchuck

Does it seem pretty accurate?

For qualitative tests it is OK.
I attach an image showing a "lane" of my testplate for the industrial hemp RSO.
One can easily see the various "blobs" and their relative size. From this lane you see that CBD is the major cannabinoid, that the THC spot is about half the size of the CBD one, there is CBG and CBC and there are some acids.
Should you want a more precise evaluation of the ratio's you can feed this image into analysis software like justquantify free which can produce a nice graphical representation.

For quantitative tests it becomes a different game.
There are many possible error sources in the process.
For example, the attached lane was obtained from a 20 mg sample of RSO.
When you ever tried to get 20 mg of tar in an Eppendorf tube you know how difficult that is.
Moreover, I used a jeweler scale with a resolution of 1 mg. So when it indicates 20 mg it can be anything between 19.6 and 20.4 mg. That translates into a possible error in the end result of five times this range. There are more of these potential errors along the way, so in the end I am satisfied when I have the first digit of a two-digit number correct.

Originally Posted by oldchuck

Can a technically challenged old guy figure out how to use it?

I would think so.
Montana Biotech placed some clips on youtube, showing their entire process.
I used a different testkit but the technique is similar.

Originally Posted by oldchuck

One other question, do you happen to know the variety of hemp you extracted.

No, I don't.
I did not bother to test the dried buds either, I jumped straight into the RSO extraction.

Hope this helps

[oldchuck]

Thank you, Herm.

[Only Ornamental]

Originally Posted by oldchuck

Trucker, I think ditchweed is VERY valuable. But, obviously, not for getting high. I suspect the ditchweed gene pool is a lost treasure that needs to be recovered. Fortunately, Cannabis, is a remarkable survivor plant. It adapts to almost anything. Ditchweed is feral which means it escaped from cultivation and has learned to get along without human intervention. The genes are all still there. I think it is mostly descended from the legendary Kentucky hemp of the 19th century which was improved through the 1920s and spread all over the midwest.

I want seeds. Send me seeds and lets see if we can revive Kentucky hemp and improve it. I want to make a strain good for fiber uses, food uses, and high CBD for medicinal uses.

Seeds, I want ditchweed seeds. Screw THC. You can get that anywhere.

Agree 101%!

I see potential in hemp only because of its hardiness, adaptation to European/US climate, pest resistance, different 'chemical space' (other secondary cannabinoids and terpenes than drug type cannabis) and different genetic background and heterogeneity maybe adding to the heterosis effect when attempting a true breeding crosses, and it also has 'sentimental' values (biodiversity).

But for CBD it'll be easier with a charas or hashish cultivar which often have CBD individuals. Look at The Real Seed Company! Great unworked and hardy landraces.

Have a look at THIS THREAD, post #113 and following, for infos on DIY TLC .

[oldchuck]

I hear you on the CBD, OO. She is an elusive bitch. I'll need to be able to do testing before I can get anywhere with Miss CBD. I ordered one of those MYDx gadgets but I won't be getting it until Christmas.

Today I am attending a day long workshop on oilseeds which will include hemp. I will be asking our University Ag people about getting legal seed through them thanks to the new Farm Bill rules.

[Only Ornamental]

You could also do a Beam test for THC or the Duquenois-Negm test for CBD. Basically, they show just presence or absence of the two...
KLICK ME

Have fun on your workshop!

[oldchuck]

I can test THC well enough with my brain.

Later

[amannamedtruth]

You can buy high CBD oil on Ebay.

https://www.ebay.com/itm/like/321312985174?lpid=82

and lots of other places, too.

[Rinse]

Originally Posted by amannamedtruth

You can buy high CBD oil on Ebay.

https://www.ebay.com/itm/like/321312985174?lpid=82

and lots of other places, too.

If you smoked good weed after you take that stuff will you get high?
Could be used as an "antidote" for one toke over the line.