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Old 05-26-2012, 12:29 PM #1
Sam_Skunkman
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HOW OLD CAN POLLEN BE ALIVE?

How long can you keep pollen alive and still get pollination?
How did you keep the pollen?

-SamS
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Old 05-26-2012, 01:02 PM #2
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hi sam, i keep mine in ''00'' gel caps that i get from the chemist. i keep them in the fridge. the gel cap takes the moisture out of the pollen and than the fridge takes it out of the gel cap. i have had pollen that is still viable after two years. only take it out of the fridge when you need it though or else it will die.
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Old 05-30-2012, 08:20 AM #3
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I have some Original Haze pollen that is 17 years old, collected, cleaned, dried over night, placed in a paper bindle's, and frozen in glass jars for 17 years.
When I used it recently I got normal seed set.
I have many, many, liters of pollen frozen, the oldest around 20+ years.

-SamS
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Old 05-30-2012, 09:51 AM #4
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5 days hanging around in the Postal system in warm Spain seemed to do no harm, kept in a Rizzla paper in a fridge with Silica desiccant for months before and after.
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Old 05-30-2012, 09:55 AM #5
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Quote:
Originally Posted by Sam_Skunkman View Post
I have some Original Haze pollen that is 17 years old, collected, cleaned, dried over night, placed in a paper bindle's, and frozen in glass jars for 17 years.
When I used it recently I got normal seed set.
I have many, many, liters of pollen frozen, the oldest around 20+ years.

-SamS
17 years thats awesome

do you keep any kind of desiccant in the jars?

peace,
Infi
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Old 05-30-2012, 09:55 AM #6
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mixed with flour in a baby food jar in a box room temp possibly warmer for 4 years now. still viable.
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Old 05-30-2012, 11:25 AM #7
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If I may say, i'm very septic about pollen being still viable after more than 3-4 years especially with the storage described by each of you. Pollen viability of the vast majority of angiospermes decrease to arround 10% a few hours after stamen opening. With adequate storage, you may be able to keep it a few years, no more.

did anybody made it germinate on water-sucrose agar and check the emergence of germtubes with microscope ? be careful because the seeds may be parthenocarpic , the developement being triggered by contact between the dead pollen and the pistil and/or aborted germination of non viable pollen.

the best way is still to keep the pollen in vacuum sealed vials at -18°C (see post i did a few month ago on mrnice forum)

https://www.mrnice.nl/forum/6-breeder...le-pollen.html

good luck
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Old 05-30-2012, 07:41 PM #8
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im not a lab assistant im just telling you what i did and that it still pollinated my plant and more branches than i intended.

it made seeds. experiment done.
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Old 05-30-2012, 07:55 PM #9
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ive found that mine (properly dried) only lasts for a year or so in a regular 'frost free' freezer. frost free freezers partially defrost to stop the ice build up so this probably compromises the storage somewhat.

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Old 05-30-2012, 08:00 PM #10
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Originally Posted by Sam_Skunkman View Post
I have some Original Haze pollen that is 17 years old
Was the male spicy/incense smell? Too much bland sweet haze these days it seems... Nevilles haze still got the church (chaco's 130day pheno)

Peace babeh
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