Standards Used in Testing - Cannabis Botany and Advanced Growing Science - International Cannagraphic Magazine Forums

Green Supreme · 12-19-2011, 06:14 PM

So I see all these people so firm in their stance for one testing facility or the other. My questions would be, where did these places acquire the standards to test against? Especially those in the US. Can we really believe these tests? Just wondering how so many folks can put faith in such things. Standards are not easy to come by. Peace GS

ps. apologies if this is in the wrong forum, if so feel free to move

Gray Wolf · 12-19-2011, 07:36 PM

I just glimpsed this post and not only applaud the question, but have some related questions to add. Please excuse my ignorance on any really dumb ones!

As a manufacturing engineer, I selected and installed automatic GC analysis equipment, and without exception, the automated equipment I installed, required a standard signature programmed into them, to be able to sort out the results from the test.

In keeping with that limited experience, my negotiations with a local lab for shared research fell through as the details unfolded, because they also need standards. When I asked for it to be tested for THC, CBD, and CBN, there was no problem and the owner proudly noted that he belonged to an association and they compared and shared standards.

The problem began when I asked if he could also do THCA and he said he would need another standard and then I started talking about the other cannabinoids, turpenoids and residual solvent, and contaminants in the solvent, and pesticides, and very quickly all my shared research plans fell apart from unattractive costs and lack of standards. He was willing to do anything I wanted, but it required another test and or standard, that I specifically had to specify, if I wanted it done.

I am especially interested in their standards for all the turpenoids known to be present in cannabis, as well as what contaminants they automatically check for.

I would also be interested in how many GC samples they run to positively identify and measure constitutes in the low PPM and PPB range?

Any of ya'll experts on GC and HPLC care to share your thoughts on the above?

Rusty420 · 12-19-2011, 08:51 PM

sam skunkman will know..

Green Supreme · 12-20-2011, 01:32 AM

Ya GW, you see where I am going. Peace GS

Green Supreme · 12-20-2011, 08:21 PM

Well I seriously thought this would inspire more debate. Where are all those test braggers now? Peace GS

highonmt · 12-22-2011, 06:18 PM

Quote:

Originally Posted by Green Supreme

Well I seriously thought this would inspire more debate. Where are all those test braggers now? Peace GS

Here
https://www.usp.org/referenceStandards/
Probably checking out the reference standards provided by the dorky chick in the hair net. Seriously you just purchase standards and run a standard then run your tests followed by another run of the standard to verify. Without a standard of known concentration quantification is very difficult. With a standard you just calculate the area under the peak of the standards and then compare it to the peak of your analyte. Retention times of the standards tell you what is coming off the column...easy as pie no need for debate..HM

Green Supreme · 12-22-2011, 06:23 PM

Still wondering where these places everyone banks on, like Steephill get their standards. Peace GS

Gray Wolf · 12-22-2011, 06:32 PM

Quote:

Originally Posted by highonmt

Here
https://www.usp.org/referenceStandards/
Probably checking out the reference standards provided by the dorky chick in the hair net. Seriously you just purchase standards and run a standard then run your tests followed by another run of the standard to verify. Without a standard of known concentration quantification is very difficult. With a standard you just calculate the area under the peak of the standards and then compare it to the peak of your analyte. Retention times of the standards tell you what is coming off the column...easy as pie no need for debate..HM

Thanks for the link brother HOM; I will check their product line out.

Do you have any insight into the rest of the above questions?

Gray Wolf · 12-22-2011, 06:47 PM

Yup, OK I did; have you read their catalog? It doesn't appear to include cannabinoids or any of the principal turpenoids?

legalizeDK · 12-22-2011, 06:54 PM

GW i know i litle bit about ms maybe i can help but i am unsure of waht you want to know

12-19-2011, 06:14 PM	#1
Green Supreme Banned Join Date: Aug 2011 Location: heaven, B.C. Posts: 5,105	Standards Used in Testing So I see all these people so firm in their stance for one testing facility or the other. My questions would be, where did these places acquire the standards to test against? Especially those in the US. Can we really believe these tests? Just wondering how so many folks can put faith in such things. Standards are not easy to come by. Peace GS ps. apologies if this is in the wrong forum, if so feel free to move
	Quote 5 members found this post helpful.

12-19-2011, 07:36 PM	#2
Gray Wolf A Posse ad Esse. From Possibility to realization. Join Date: Apr 2009 Location: River City, USA Posts: 9,402	I just glimpsed this post and not only applaud the question, but have some related questions to add. Please excuse my ignorance on any really dumb ones! As a manufacturing engineer, I selected and installed automatic GC analysis equipment, and without exception, the automated equipment I installed, required a standard signature programmed into them, to be able to sort out the results from the test. In keeping with that limited experience, my negotiations with a local lab for shared research fell through as the details unfolded, because they also need standards. When I asked for it to be tested for THC, CBD, and CBN, there was no problem and the owner proudly noted that he belonged to an association and they compared and shared standards. The problem began when I asked if he could also do THCA and he said he would need another standard and then I started talking about the other cannabinoids, turpenoids and residual solvent, and contaminants in the solvent, and pesticides, and very quickly all my shared research plans fell apart from unattractive costs and lack of standards. He was willing to do anything I wanted, but it required another test and or standard, that I specifically had to specify, if I wanted it done. I am especially interested in their standards for all the turpenoids known to be present in cannabis, as well as what contaminants they automatically check for. I would also be interested in how many GC samples they run to positively identify and measure constitutes in the low PPM and PPB range? Any of ya'll experts on GC and HPLC care to share your thoughts on the above? __________________ An age is called Dark not because the light fails to shine, but because people refuse to see it. Believing is seeing and ignorance is bliss until it bites you in the ass! Fervor is the weapon of the impotent. The harder the sell, the poorer the product. Alas, my ignorance abounds; the more I've learned, the less I know that I know.......... Thou shalt seek and respect the opinions of operators, even unto the third helper, for theirs is a wisdom unknown to technicrats. Wise men learn more from fools, than fools from wise men. In my dotage I finally discovered that the secret to putting on pants both legs at a time is sitting down.
	Quote 4 members found this post helpful.

12-22-2011, 06:47 PM	#9
Gray Wolf A Posse ad Esse. From Possibility to realization. Join Date: Apr 2009 Location: River City, USA Posts: 9,402	Yup, OK I did; have you read their catalog? It doesn't appear to include cannabinoids or any of the principal turpenoids? __________________ An age is called Dark not because the light fails to shine, but because people refuse to see it. Believing is seeing and ignorance is bliss until it bites you in the ass! Fervor is the weapon of the impotent. The harder the sell, the poorer the product. Alas, my ignorance abounds; the more I've learned, the less I know that I know.......... Thou shalt seek and respect the opinions of operators, even unto the third helper, for theirs is a wisdom unknown to technicrats. Wise men learn more from fools, than fools from wise men. In my dotage I finally discovered that the secret to putting on pants both legs at a time is sitting down.
	Quote

12-22-2011, 06:54 PM	#10
legalizeDK Member Join Date: Jun 2009 Location: up north Posts: 539	GW i know i litle bit about ms maybe i can help but i am unsure of waht you want to know __________________ "Those who desire to give up freedom in order to gain security will not have, nor do they deserve, either one." Benjamin Franklin
	Quote

12-19-2011, 08:51 PM	#3
Rusty420 Banned Join Date: Aug 2010 Posts: 729	sam skunkman will know..
	Quote

12-20-2011, 01:32 AM	#4
Green Supreme Banned Join Date: Aug 2011 Location: heaven, B.C. Posts: 5,105	Ya GW, you see where I am going. Peace GS
	Quote

12-20-2011, 08:21 PM	#5
Green Supreme Banned Join Date: Aug 2011 Location: heaven, B.C. Posts: 5,105	Well I seriously thought this would inspire more debate. Where are all those test braggers now? Peace GS
	Quote

12-22-2011, 06:23 PM	#7
Green Supreme Banned Join Date: Aug 2011 Location: heaven, B.C. Posts: 5,105	Still wondering where these places everyone banks on, like Steephill get their standards. Peace GS
	Quote