Tissue Culture (New age Cloning ) - Cannabis Botany and Advanced Growing Science - International Cannagraphic Magazine Forums

DCgrow505 · 10-02-2011, 01:37 PM

Tissue Culture (New age Cloning )

Whats going on my fellow Green Grower's . So I have been doing a bit of research on TC cloning and it really sounds cool and i can't wait to try it . So what the hell is tissue cloning and why should I care . At its called micro cloning because true TC cloning is done in a lab and is really tedious . Micro cloning is sort of like normal cloning but a lot more sterile and on a miniature scale in jars. Its quickly becoming the way that most clones are made and genetics are kept . Which is why you should care (as a example almost all of the plants a home depot are TC .Think limitless number of plants that are all identical but without the huge mother rooms and problems that were all used to .
The process in a nutshell is this Take small tip cuttings from a mother plant and wash them thoroughly with soap and water then alcohol the diluted bleach without roots as a micro bonsai. . Whats special gelatin . It come in TC kits Official Its purified water standard nutrient salts , vitamins and amino acids, branching hormone, preservative, and gellan gum to make it all gelatinous.. Every five weeks or so, the cultures woo grow from one node to approximately five and then are cut apart and put into five new jars . This is the multiplication stage repeat as necessary until you have enough new moms to supply your needs. Whenever you need clones, you cut it into five parts as before , then put them in a jar with rooting hormones instead of branching hormones and wait two weeks . At that point its time to move them to a standard clone tray with grow medium and a dome to let them plump up a bit from one inch to more like five . You now have a whole tray of beautiful clones that will grow into large productive plants.
Now Here's some of the pro's of (TC)
Keeps 100 strains on a shelf under a single T5 and you only have to re-jar every five to eight weeks .
Watering is no longer needed...........Crazy right
Genetic drift is eliminated , and even damaged genetics can be brought bavk to their original vigor .
The wattage used for mothers and clones is greatly reduced.
Bugs and mold are no longer part of the process.
There is no other practical way to provide high numbers of clones (thousands at a time)that all short with a high node count and at the same growth stage .
Which is perfect for a sea of green setup or rotating gardens.

Now HERE'S THE CONS
Tot have to plan five weeks ahead,versus two for traditional cloning .
There's a 10 week initial lead time to deal with.
It's a cleanly process,so if you have an aversion to being clean, may have some problems.
There are places out there that supply all of this stuff in kit form .One is pure food Gardening , which you can find at planttc.com
Now there you have it its probly the coolest and best way to take clones . Now has any one done this or have one of the kits to do it .Cause im going to be getting one and would like to hear any tips or coments about it and im going to be documenting it and updating this thread so we can how it works out .Cause i have a fuck load of strains and really don't have the room for mothers any more and this way i will be able to keep all my good genetics cause lets face its hard to let go of a damn good strain just cause you got a new one that you want to try but dont have the room for a other mother so its ether one or the other . Now i can not only keep all my strain but i can start getting more and just store them and start them back up when ever i feel like it . Sound's like its worth a try shit . What do you guys think and it

sneaky101 · 10-02-2011, 02:05 PM

Tagged.

Hi Dc,I saw this a year or two ago and was very interested for the same reason! But was busy learning the skills of this trade...baby steps. As you document this process, please include where to purchase (as you have already started) if it is allowable on this forum! And also the costs of the items needed. I am very interested on learning this process and since someone else is going through the motions, it may motivate me to pick this back up.

Good luck!

jgrow · 10-03-2011, 06:14 PM

This could be amazing. Im in!

spurr · 10-03-2011, 08:54 PM

Gray Wolf and GreenintheThumb and Chimera are the folks you want to talk to, as well as myself but I haven't actually done any TC work on cannabis yet, and they all have. If you're interest in TC you should join my Blockhead Society group, wherein you will find good info on TC and related topics. A great thread that is chalk full of great info is from Gray Wolf in this sub-forum: Cannabis tissue culture

P.S. The search engine is your friend

dbcooper · 10-04-2011, 03:20 AM

Always thought this was interesting after I saw a show on a farmer that used this method, when I asked around it got the cold shoulder, guess it was to new.

TheNewGuy · 10-23-2011, 01:59 AM

AVG popped up while going to this planttc.com page recommended from the user above, don't go there.

Voidling · 10-23-2011, 02:11 AM

In a thread on this sight that had a pdf where the Chinese used TC on hemp and listed the ingredients they found to work best.

TC is in line after my led lights

Stress_test · 10-23-2011, 02:54 AM

planttc.com is a good site.

I've been researching this idea for several months now. I even ordered a complete kit from planttc.com and kinda plan on starting some hands on with it this winter.

Voidling · 10-23-2011, 03:17 AM

I hope you take detailed notes to share.

wantaknow · 10-23-2011, 03:53 AM

its old school

10-02-2011, 01:37 PM	#1
DCgrow505 New Member Join Date: Jun 2011 Posts: 12	Tissue Culture (New age Cloning ) Tissue Culture (New age Cloning ) Whats going on my fellow Green Grower's . So I have been doing a bit of research on TC cloning and it really sounds cool and i can't wait to try it . So what the hell is tissue cloning and why should I care . At its called micro cloning because true TC cloning is done in a lab and is really tedious . Micro cloning is sort of like normal cloning but a lot more sterile and on a miniature scale in jars. Its quickly becoming the way that most clones are made and genetics are kept . Which is why you should care (as a example almost all of the plants a home depot are TC .Think limitless number of plants that are all identical but without the huge mother rooms and problems that were all used to . The process in a nutshell is this Take small tip cuttings from a mother plant and wash them thoroughly with soap and water then alcohol the diluted bleach without roots as a micro bonsai. . Whats special gelatin . It come in TC kits Official Its purified water standard nutrient salts , vitamins and amino acids, branching hormone, preservative, and gellan gum to make it all gelatinous.. Every five weeks or so, the cultures woo grow from one node to approximately five and then are cut apart and put into five new jars . This is the multiplication stage repeat as necessary until you have enough new moms to supply your needs. Whenever you need clones, you cut it into five parts as before , then put them in a jar with rooting hormones instead of branching hormones and wait two weeks . At that point its time to move them to a standard clone tray with grow medium and a dome to let them plump up a bit from one inch to more like five . You now have a whole tray of beautiful clones that will grow into large productive plants. Now Here's some of the pro's of (TC) Keeps 100 strains on a shelf under a single T5 and you only have to re-jar every five to eight weeks . Watering is no longer needed...........Crazy right Genetic drift is eliminated , and even damaged genetics can be brought bavk to their original vigor . The wattage used for mothers and clones is greatly reduced. Bugs and mold are no longer part of the process. There is no other practical way to provide high numbers of clones (thousands at a time)that all short with a high node count and at the same growth stage . Which is perfect for a sea of green setup or rotating gardens. Now HERE'S THE CONS Tot have to plan five weeks ahead,versus two for traditional cloning . There's a 10 week initial lead time to deal with. It's a cleanly process,so if you have an aversion to being clean, may have some problems. There are places out there that supply all of this stuff in kit form .One is pure food Gardening , which you can find at planttc.com Now there you have it its probly the coolest and best way to take clones . Now has any one done this or have one of the kits to do it .Cause im going to be getting one and would like to hear any tips or coments about it and im going to be documenting it and updating this thread so we can how it works out .Cause i have a fuck load of strains and really don't have the room for mothers any more and this way i will be able to keep all my good genetics cause lets face its hard to let go of a damn good strain just cause you got a new one that you want to try but dont have the room for a other mother so its ether one or the other . Now i can not only keep all my strain but i can start getting more and just store them and start them back up when ever i feel like it . Sound's like its worth a try shit . What do you guys think and it
	Quote

10-02-2011, 02:05 PM	#2
sneaky101 Member Join Date: Nov 2009 Posts: 919	Tagged. Hi Dc,I saw this a year or two ago and was very interested for the same reason! But was busy learning the skills of this trade...baby steps. As you document this process, please include where to purchase (as you have already started) if it is allowable on this forum! And also the costs of the items needed. I am very interested on learning this process and since someone else is going through the motions, it may motivate me to pick this back up. Good luck!
	Quote

10-03-2011, 06:14 PM	#3
jgrow Member Join Date: Mar 2009 Posts: 480	This could be amazing. Im in! __________________ and we all can smoke two pounds of cali herb by ourselves then try to stand on our heads no matter how high we get we'll never forget the wonders of mary's bed and we can run 200 miles through a ganja feild until all we see is red and we can shout it out we're still thinking about the wonders of mary's bed and so we sing jump around sweet mary you know we all have to move along you never know what you've got in this cruel world until you realize its gone
	Quote

10-03-2011, 08:54 PM	#4
spurr Banned Join Date: Aug 2010 Location: https://www.scirus.com/ & https://www.google.com/schhp?hl=en Posts: 2,431	Gray Wolf and GreenintheThumb and Chimera are the folks you want to talk to, as well as myself but I haven't actually done any TC work on cannabis yet, and they all have. If you're interest in TC you should join my Blockhead Society group, wherein you will find good info on TC and related topics. A great thread that is chalk full of great info is from Gray Wolf in this sub-forum: Cannabis tissue culture P.S. The search engine is your friend
	Quote

10-04-2011, 03:20 AM	#5
dbcooper Member Join Date: Mar 2007 Posts: 69	Always thought this was interesting after I saw a show on a farmer that used this method, when I asked around it got the cold shoulder, guess it was to new.
	Quote

10-23-2011, 01:59 AM	#6
TheNewGuy Member Join Date: Jul 2010 Posts: 68	AVG popped up while going to this planttc.com page recommended from the user above, don't go there.
	Quote

10-23-2011, 02:11 AM	#7
Voidling Member Join Date: Dec 2010 Posts: 265	In a thread on this sight that had a pdf where the Chinese used TC on hemp and listed the ingredients they found to work best. TC is in line after my led lights
	Quote

10-23-2011, 02:54 AM	#8
Stress_test I'm always here when I'm not someplace else Join Date: Oct 2010 Posts: 2,527	planttc.com is a good site. I've been researching this idea for several months now. I even ordered a complete kit from planttc.com and kinda plan on starting some hands on with it this winter. __________________ "I would. I'd really like to meet an honest man. But I'd have to lie to him cause I smoke pot." ______________________________ ______ First Outdoor Guerrilla Grow 2011 Understanding Ph. Ingenious $4 Walmart Cloner. SOG's DIY micro-cloning factory tutorial. (Very well illustrated and explained). Handy site for the Gardener (A wealth of information for any gardener). Sometimes when I'm really medicated, I can really crack me up.
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10-23-2011, 03:17 AM	#9
Voidling Member Join Date: Dec 2010 Posts: 265	I hope you take detailed notes to share.
	Quote

10-23-2011, 03:53 AM	#10
wantaknow ruger 500 Join Date: Apr 2004 Location: usa Posts: 2,100	its old school __________________ Free leonard peltier now,
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