Cannabis tissue culture - Cannabis Botany and Advanced Growing Science - International Cannagraphic Magazine Forums

Gray Wolf · 03-31-2011, 02:09 PM

Here are some purdy ladies grown from meristematic cells in a special medium by our inhouse red headed geek, instead of seeds or clones. The meristematic cells are extracted form the stem nodes anywhere on the mother plant.

One of the advantages is that no diseases or systemic parasites are passed on.

Hee, hee, hee, snicker, snark, snort.....................

PistilPete · 03-31-2011, 02:15 PM

Excellent. Never seen it done before with mj. Good luck with the trials. I'll be watching and trying to learn a thing or 3.

03-31-2011, 02:36 PM

Sweet! I have wanted to mess with this for a while. Any more pix and could you elaborate more bro!!

bloyd · 03-31-2011, 04:20 PM

Awesome gray wolf and growgeek! Have you guys looked into making artificial seed at all? Would love any details you have.

ILoveit · 03-31-2011, 05:09 PM

Wow Im intrigued, please keep us posted.

Sheriff Bart · 03-31-2011, 05:40 PM

plants dont have 'stem cells' so to speak. plants contain meristematic tissues, which are the tissues in the plant which are actively dividing and growing. after they divide differentiation occurs, where the cells which were once just dividing cells, start to do different things, like turn into mesophyll cells (photosynthesis) or vascular tissues(transport), etc. etc. the majority of plant cells are also totipotent, that is that they have the ability to de-differentiate from whatever tissue they were found in and can start to divide again like the meristematic tissue and grow what is known as callous tissue. you can control what organs callous tissue will differentiate out into with hormone treatments. the auxins (IAA, IBA, NAA, 2,4-D) will be used to induce root growth and the cytokinins (BAP, kinetin, zeatin, etc.) can be used to induce new plants shoots to grow off the callous tissue which can be cut and rooted into new plants (or roots can be grown in the tc and the plants can be transplanted)

it looks like though that your geek was simply tissue culturing nodes with meristems (shoots) already on them, and not doing tc from a leaf, or pith or some other single tissue culture, but a mixed tissue culture so theres probably just regular nutrients in the agar and no hormones.

and r.e. the pathogens. the theory is that viruses either cannot or do not penetrate into the meristematic tissues of the plants (because if they did they would kill the plant way faster) so you can TC the meristematic tissues and hopefully will clone off virus free growth.

ScrubNinja · 03-31-2011, 05:47 PM

Very cool, cheers for posting. Do you have a full blown lab, or how much trouble/expense did you go to to create a suitable environment to do this?

maxmurder · 03-31-2011, 05:57 PM

Quote:

Originally Posted by ScrubNinja

Very cool, cheers for posting. Do you have a full blown lab, or how much trouble/expense did you go to to create a suitable environment to do this?

very interested- thanks for posting this

420247 · 03-31-2011, 07:35 PM

Anybody know anything about Cybridization/Somatic hybridization?

I'd love the learn more about this

https://www.icmag.com/ic/showthread.php?t=204436

^I posted a some info I found about this^

Gray Wolf · 03-31-2011, 07:36 PM

Quote:

Originally Posted by bloyd

Awesome gray wolf and growgeek! Have you guys looked into making artificial seed at all? Would love any details you have.

Yup, we do have plans for artificial seeds, but haven't tested out our theories yet, so let us get some tangible results before commenting. If it works, I will ask the geekmiester to publish a report.

These are also amongst the first two dozen strains that he cultured, and so far he has grown no roots (making these non plants) on any of them, or raised any through adult hood. I shared his tissue culture results early, because the results to date have been so encouraging.

I will continue to share pictures as they progress through adulthood and if this experiment finishes as well as it started, I will ask him to publish his notes as well.

03-31-2011, 02:09 PM	#1
Gray Wolf A Posse ad Esse. From Possibility to realization. Join Date: Apr 2009 Location: River City, USA Posts: 9,402	Cannabis tissue culture Here are some purdy ladies grown from meristematic cells in a special medium by our inhouse red headed geek, instead of seeds or clones. The meristematic cells are extracted form the stem nodes anywhere on the mother plant. One of the advantages is that no diseases or systemic parasites are passed on. Hee, hee, hee, snicker, snark, snort..................... Attached Thumbnails __________________ An age is called Dark not because the light fails to shine, but because people refuse to see it. Believing is seeing and ignorance is bliss until it bites you in the ass! Fervor is the weapon of the impotent. The harder the sell, the poorer the product. Alas, my ignorance abounds; the more I've learned, the less I know that I know.......... Thou shalt seek and respect the opinions of operators, even unto the third helper, for theirs is a wisdom unknown to technicrats. Wise men learn more from fools, than fools from wise men. In my dotage I finally discovered that the secret to putting on pants both legs at a time is sitting down. Last edited by Gray Wolf; 03-31-2011 at 08:05 PM.. Reason: Change stem cells to meristematic cells
	Quote 24 members found this post helpful.

03-31-2011, 02:15 PM	#2
PistilPete Enjoying the ride Join Date: Nov 2008 Posts: 1,481	Excellent. Never seen it done before with mj. Good luck with the trials. I'll be watching and trying to learn a thing or 3. __________________ ---------------------------------------------------------------------------------------------------------- "If a law is unjust, a man is not only right to disobey it, he is obligated to do so." - Thomas Jefferson "As long as I'm alive I'm gonna live illegal" "The laws I break aren't fit to be laws in the first place." *Connoisseur Genetics - ECSD x SSSDH Testers Fems* (June 2013) ---> https://www.icmag.com/ic/showthread....84#post5809184 Connoisseur Genetics - White Jones Testers Fems (Casey Jones x The White) (Jan. 2012) ---> https://www.icmag.com/ic/showthread.php?t=231004 Pistil Pete's Tents ---> https://www.icmag.com/ic/showthread.php?t=225350 (~):} Forever Grateful {:(~)**
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03-31-2011, 05:40 PM	#6
Sheriff Bart Deputy Spade Join Date: Jun 2007 Location: Rock Ridge Posts: 1,130	plants dont have 'stem cells' so to speak. plants contain meristematic tissues, which are the tissues in the plant which are actively dividing and growing. after they divide differentiation occurs, where the cells which were once just dividing cells, start to do different things, like turn into mesophyll cells (photosynthesis) or vascular tissues(transport), etc. etc. the majority of plant cells are also totipotent, that is that they have the ability to de-differentiate from whatever tissue they were found in and can start to divide again like the meristematic tissue and grow what is known as callous tissue. you can control what organs callous tissue will differentiate out into with hormone treatments. the auxins (IAA, IBA, NAA, 2,4-D) will be used to induce root growth and the cytokinins (BAP, kinetin, zeatin, etc.) can be used to induce new plants shoots to grow off the callous tissue which can be cut and rooted into new plants (or roots can be grown in the tc and the plants can be transplanted) it looks like though that your geek was simply tissue culturing nodes with meristems (shoots) already on them, and not doing tc from a leaf, or pith or some other single tissue culture, but a mixed tissue culture so theres probably just regular nutrients in the agar and no hormones. and r.e. the pathogens. the theory is that viruses either cannot or do not penetrate into the meristematic tissues of the plants (because if they did they would kill the plant way faster) so you can TC the meristematic tissues and hopefully will clone off virus free growth. __________________ I am only here to entertain the most absurd of ideas
	Quote 19 members found this post helpful.

03-31-2011, 05:47 PM	#7
ScrubNinja Grow like nobody is watching Join Date: Mar 2008 Location: Right at the herp of the derp Posts: 5,006	Very cool, cheers for posting. Do you have a full blown lab, or how much trouble/expense did you go to to create a suitable environment to do this? __________________ Chillin' so hard my ass almost froze off 600w vert: https://www.icmag.com/ic/showthread.php?t=236394
	Quote 1 members found this post helpful.

03-31-2011, 07:35 PM	#9
420247 Plant Whisperer Join Date: Oct 2004 Posts: 1,275	Anybody know anything about Cybridization/Somatic hybridization? I'd love the learn more about this https://www.icmag.com/ic/showthread.php?t=204436 ^I posted a some info I found about this^ __________________ I am the plant whisperer!
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03-31-2011, 02:36 PM	#3
chefro420 Guest Posts: n/a	Sweet! I have wanted to mess with this for a while. Any more pix and could you elaborate more bro!!
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03-31-2011, 04:20 PM	#4
bloyd Member Join Date: Jan 2010 Posts: 738	Awesome gray wolf and growgeek! Have you guys looked into making artificial seed at all? Would love any details you have.
	Quote

03-31-2011, 05:09 PM	#5
ILoveit Member Join Date: Jun 2009 Posts: 273	Wow Im intrigued, please keep us posted.
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