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PhenoMenal · 02-14-2018, 01:18 PM

Oxossi, on behalf of every MMJ patient, thankyou so much for sharing your experience and knowledge -- TLC is such a fantastic tool for medical users wondering "what's in this?", but it's still rare to come across people like yourself who actually 1) know what they're talking about, and 2) are generous enough to share their knowledge

Very much appreciated mate, thankyou

dybert · 02-21-2018, 06:29 PM

This is a plate I found in my ICmag pictures... It was from an MZ12X extraction, but you can see the good separation we achieve with chloroform...

dongle · 03-04-2018, 05:08 AM

What did you use as the developing dye?

I faintly recall Fast Blue B or Fast Blue BB being one of the few suitable dyes.

Wondering if you found any different?

I vaguely remember that one was better than the other at distinguishing various cannabinoids such as CBD vs THC vs THCa

dongle · 03-04-2018, 05:09 AM

What are the numbers written underneath each cannabinoid column?

dybert · 03-07-2018, 07:23 AM

Quote:

Originally Posted by dongle

What did you use as the developing dye?

I faintly recall Fast Blue B or Fast Blue BB being one of the few suitable dyes.

Wondering if you found any different?

I vaguely remember that one was better than the other at distinguishing various cannabinoids such as CBD vs THC vs THCa

Fast Blue B was used... the numbers are the quantifications.

Ringodoggie · 03-25-2018, 08:29 PM

Have any of you tried the plates that use a black light and do not use dye?

PhenoMenal · 03-29-2018, 02:08 PM

I took early samples during this grow, and I was stunned that the cannabinoid profile seems to be established possibly from day 1 ...

I took samples from seedlings that were only 41 and 27 days (the 41 day one shown in this) from seed - literally 41 days old from the first time water hit their seed shell ... I wasn't even sure I'd be able to see anything so early on (again this was just early VEG phase - not flowering). The 27-day one (a different strain) also showed true. I'll upload the full TLC analysis soon

It seems that Thin Layer Chromatography of a sample from a plant just a few weeks into life from seed, during veg phase, can determine the approximate cannabinoid profile at harvest.

If this is the case, breeders looking for (example) CBD don't need to spend months growing a plant and then testing it - they can test it as soon as it becomes a viable seedling.

My only question now is HOW early??? ... i have test results here saying it's gotta be before both 41 and 27 days.
... could the first-grown true/non-cotyledon leaves show the cannabinoid profile of the resulting profile at harvest?

dybert · 03-29-2018, 10:48 PM

Quote:

Originally Posted by PhenoMenal

I took early samples during this grow, and I was stunned that the cannabinoid profile seems to be established possibly from day 1 ...

View Image

I took samples from seedlings that were only 41 and 27 days (the 41 day one shown in this) from seed - literally 41 days old from the first time water hit their seed shell ... I wasn't even sure I'd be able to see anything so early on (again this was just early VEG phase - not flowering). The 27-day one (a different strain) also showed true. I'll upload the full TLC analysis soon

It seems that Thin Layer Chromatography of a sample from a plant just a few weeks into life from seed, during veg phase, can determine the approximate cannabinoid profile at harvest.

If this is the case, breeders looking for (example) CBD don't need to spend months growing a plant and then testing it - they can test it as soon as it becomes a viable seedling.

My only question now is HOW early??? ... i have test results here saying it's gotta be before both 41 and 27 days.
... could the first-grown true/non-cotyledon leaves show the cannabinoid profile of the resulting profile at harvest?

I get pretty reliable ratio results around week 3 of seedlings!

PhenoMenal · 03-30-2018, 02:15 PM

Quote:

Originally Posted by dybert

I get pretty reliable ratio results around week 3 of seedlings!

I'm guessing we can get readings from the two first true (non-cotyledon) leaves ... the only thing stopping us is that the plant needs them, lol. Let us only work from the 2nd set onwards :P

THIS IS A GAMECHANGER THOUGH!!!

dybert · 03-30-2018, 04:35 PM

Quote:

Originally Posted by PhenoMenal

I'm guessing we can get readings from the two first true (non-cotyledon) leaves ... the only thing stopping us is that the plant needs them, lol. Let us only work from the 2nd set onwards :P

THIS IS A GAMECHANGER THOUGH!!!

Its definitely nice to be able to check (especially doing CBD pheno hunting)

02-14-2018, 01:18 PM	#191
PhenoMenal XGames Quantumboarder Join Date: Oct 2006 Location: Oz Posts: 2,755	Oxossi, on behalf of every MMJ patient, thankyou so much for sharing your experience and knowledge -- TLC is such a fantastic tool for medical users wondering "what's in this?", but it's still rare to come across people like yourself who actually 1) know what they're talking about, and 2) are generous enough to share their knowledge Very much appreciated mate, thankyou __________________ ... "My job, my mission, the reason I've been put onto this planet is to save wildlife, and I thank you for coming with me" - Steve Irwin (1962-2006) Eeeasily make your own COLLOIDAL SILVER to make your own FEMINISED SEEDS! My DIY Thin Layer Chromatography @ home [link]
	Quote

02-21-2018, 06:29 PM	#192
dybert Member Join Date: Oct 2009 Location: West Coast Posts: 683	This is a plate I found in my ICmag pictures... It was from an MZ12X extraction, but you can see the good separation we achieve with chloroform...
	Quote 2 members found this post helpful.

03-29-2018, 02:08 PM	#197
PhenoMenal XGames Quantumboarder Join Date: Oct 2006 Location: Oz Posts: 2,755	I took early samples during this grow, and I was stunned that the cannabinoid profile seems to be established possibly from day 1 ... I took samples from seedlings that were only 41 and 27 days (the 41 day one shown in this) from seed - literally 41 days old from the first time water hit their seed shell ... I wasn't even sure I'd be able to see anything so early on (again this was just early VEG phase - not flowering). The 27-day one (a different strain) also showed true. I'll upload the full TLC analysis soon It seems that Thin Layer Chromatography of a sample from a plant just a few weeks into life from seed, during veg phase, can determine the approximate cannabinoid profile at harvest. If this is the case, breeders looking for (example) CBD don't need to spend months growing a plant and then testing it - they can test it as soon as it becomes a viable seedling. My only question now is HOW early??? ... i have test results here saying it's gotta be before both 41 and 27 days. ... could the first-grown true/non-cotyledon leaves show the cannabinoid profile of the resulting profile at harvest? __________________ ... "My job, my mission, the reason I've been put onto this planet is to save wildlife, and I thank you for coming with me" - Steve Irwin (1962-2006) Eeeasily make your own COLLOIDAL SILVER to make your own FEMINISED SEEDS! My DIY Thin Layer Chromatography @ home [link]
	Quote 2 members found this post helpful.

03-04-2018, 05:08 AM	#193
dongle Newbie Join Date: Jan 2018 Posts: 5	What did you use as the developing dye? I faintly recall Fast Blue B or Fast Blue BB being one of the few suitable dyes. Wondering if you found any different? I vaguely remember that one was better than the other at distinguishing various cannabinoids such as CBD vs THC vs THCa
	Quote

03-04-2018, 05:09 AM	#194
dongle Newbie Join Date: Jan 2018 Posts: 5	What are the numbers written underneath each cannabinoid column?
	Quote

03-25-2018, 08:29 PM	#196
Ringodoggie Member Join Date: Apr 2015 Posts: 790	Have any of you tried the plates that use a black light and do not use dye?
	Quote