[NotGuilty]

Hi everyone. I plan on trying some TLC at home with hash oil. Basically I am looking for some any input/tips you have.

Here is an overview of TLC:

https://en.wikipedia.org/wiki/Thin_layer_chromatography

Here is another link on Grade 4 science using Simple TLC with Chlorophyll and one with inks:

https://www.scienceprojectlab.com/thi...-for-kids.html

https://www.sciencemadesimple.com/lea...cience_project

This explains making the plates at home:

https://blog.makezine.com/archive/200...C-0D6B48984890

Here is a quick link on uses of cannabis chromatography:

https://www.springerprotocols.com/Abs...9259-030-6:145

I plan use a method similar to the one with pens using plates instead of paper. I plan to try a long soak Iso, QWISO, BHO.

I have a few questions. And plan on trying many different ways. If the results are usable it would be neat to have a 3'x3' "plate", cut out sections and extract the separate compounds.

better hurry up with that camera.

[Payaso]

So, many questions....

Using this for potency testing? Looking for contaminants?

Just two so far!

Very interesting indeed.

Keep us posted!

[NotGuilty]

Payaso, Potency test yes, not 100% accurate but can get a rough idea? That is IF it even works out. I have a feeling with so many cannabinoids all so similar, It will not look as Clean as the Ink demo. Temp will play a big role here i suspect, by altering viscosities and all that buisness. And I may even need to add a reagent to show results visually.

IF it even works......

[ganja din]

Hey notguilty,

Give me an hour or so and ill post how *semi-quantitative* (comparative) TLC is carried out using Fast blue BB reagents. I have researched this for a while ago and have it all figured out.

For true quantitative testing one needs standards of cannabinoids in question. I should have access to standards in the next 6 months and ill share for free

For now, check out "JustTLC" and "JustQuantify" (free online tool).

HTH

[NotGuilty]

Thanks ganja din. I appreciate you stopping in. I would love to see this made practical for everyone.


Briefly skimmed over "just TLC", basically a software that does what I had planned. My crude method was going to be to scan the result and figure surface area/volume of the compounds in relation to each other to get a rough idea of the concentrations... using pc scanner and photoshop or something.

[ganja din]

Hey notguilty,

Scanning spot density is the best method, one can use Photoshop to do the same, or the free JustQuantify, or the great JustTLC (makers of JustQuantify).

A standard is >98% pure cannabinoid in question. Without a standard one can't tell how much cannabinoid is present in a sample, ex. % THC (by weight). If one does have a standard then they know the quantity (weight) of the cannabinoid in the standard. Thus by comparing spot densities one can calculate the weight of the cannabinoid in sample.

IIRC, JustTLC has been proven as accurate as HPLC if done correctly. There is a independent study on the JustTLC site with evaluates and states JustTLC for use in analytical research.

If one has no standard then one can get a baseline (average) of X samples. Then test samples and save ones which surpass the baseline. Of those, run comparative test to see which has the greatest density spot. The sample with greatest spot density = strongest sample.

Using fast blue BB reagent one can test for many cannabinoids. With use of a reagent we can use spot color as cannabinoid qualification, no need for Rf vales.

Hey! Now I think about it one could very easily use TLC for qualification of hydrocarbons in the sample! This means one can tell if/how pure their sample (ex. BHO) is. For example: one can test to see if there is presence of butane in the sample (qualification). And using a butane standard, one can tell how much butane is present in the sample (quantitication). All one needs to know is the TLC mobile phase and visualization reagent for butane.

OK. Gotta go, working on a method to make Sams secret sift...

[BigWillyDee]

read the wiki link and looks very interesting....what exactly will you be using it for...as far as cannabis goes??

[BigWillyDee]

nevermind ganja answered them.....

thats pretty cool shit..

[ganja din]

Billy,

Read my post above yours one can test bud, BHO, hash, QWISO, Bubblebag hash, etc, etc

[ganja din]

Hey notguilty,

I agree everyone should be able to do this. That was my main motivation for that project. There are kits out there like cannafingerprint, or something, but its not worth the money. My process is far superior and less expensive (after initial setup cost)